ID NIKR_PYRFU Reviewed; 138 AA.
AC Q8U3X9;
DT 20-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 27-MAR-2024, entry version 107.
DE RecName: Full=Putative nickel-responsive regulator {ECO:0000255|HAMAP-Rule:MF_00476};
GN OrderedLocusNames=PF0322;
OS Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=186497;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA DiRuggiero J., Robb F.T.;
RT "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT horikoshii inferred from complete genomic sequences.";
RL Genetics 152:1299-1305(1999).
CC -!- FUNCTION: Transcriptional regulator. {ECO:0000255|HAMAP-Rule:MF_00476}.
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00476};
CC Note=Binds 1 nickel ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00476};
CC -!- SIMILARITY: Belongs to the transcriptional regulatory CopG/NikR family.
CC {ECO:0000255|HAMAP-Rule:MF_00476}.
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DR EMBL; AE009950; AAL80446.1; -; Genomic_DNA.
DR RefSeq; WP_011011437.1; NZ_CP023154.1.
DR AlphaFoldDB; Q8U3X9; -.
DR SMR; Q8U3X9; -.
DR STRING; 186497.PF0322; -.
DR PaxDb; 186497-PF0322; -.
DR GeneID; 41712114; -.
DR KEGG; pfu:PF0322; -.
DR PATRIC; fig|186497.12.peg.337; -.
DR eggNOG; arCOG01008; Archaea.
DR HOGENOM; CLU_113319_1_2_2; -.
DR OrthoDB; 25654at2157; -.
DR PhylomeDB; Q8U3X9; -.
DR Proteomes; UP000001013; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0010045; P:response to nickel cation; IEA:InterPro.
DR CDD; cd22231; RHH_NikR_HicB-like; 1.
DR Gene3D; 3.30.70.1150; ACT-like. Chain A, domain 2; 1.
DR Gene3D; 1.10.1220.10; Met repressor-like; 1.
DR HAMAP; MF_00476; NikR; 1.
DR InterPro; IPR027271; Acetolactate_synth/TF_NikR_C.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR InterPro; IPR002145; CopG.
DR InterPro; IPR022988; Ni_resp_reg_NikR.
DR InterPro; IPR010985; Ribbon_hlx_hlx.
DR InterPro; IPR014864; TF_NikR_Ni-bd_C.
DR PANTHER; PTHR34719; NICKEL-RESPONSIVE REGULATOR; 1.
DR PANTHER; PTHR34719:SF2; NICKEL-RESPONSIVE REGULATOR; 1.
DR Pfam; PF08753; NikR_C; 1.
DR Pfam; PF01402; RHH_1; 1.
DR SUPFAM; SSF55021; ACT-like; 1.
DR SUPFAM; SSF47598; Ribbon-helix-helix; 1.
PE 3: Inferred from homology;
KW DNA-binding; Metal-binding; Nickel; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..138
FT /note="Putative nickel-responsive regulator"
FT /id="PRO_0000139311"
FT BINDING 78
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT BINDING 89
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT BINDING 91
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT BINDING 97
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
SQ SEQUENCE 138 AA; 15772 MW; 16E3FB870378950A CRC64;
MGIVRFGVSI PKELLEKFDG IIEEMGYTNR SEAIRDLIRD MVVRHEWQLG DEEVAGTITI
VYNHDEADVV RELLDLQHEY LNEIVSSLHV HMDEHNCLEV IVVKGRAKKI KKIADRLLSL
KGVKHGKLVM TTTGRDIV
//
