ID NOL9_DICDI Reviewed; 683 AA.
AC Q54Z27;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 24-JAN-2024, entry version 94.
DE RecName: Full=Polynucleotide 5'-hydroxyl-kinase nol9;
DE EC=2.7.1.-;
DE AltName: Full=Nucleolar protein 9 homolog;
GN Name=nol9; ORFNames=DDB_G0277945;
OS Dictyostelium discoideum (Social amoeba).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Polynucleotide 5'-kinase involved in rRNA processing.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Clp1 family. NOL9/GRC3 subfamily.
CC {ECO:0000305}.
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DR EMBL; AAFI02000023; EAL68145.1; -; Genomic_DNA.
DR RefSeq; XP_642025.1; XM_636933.1.
DR AlphaFoldDB; Q54Z27; -.
DR SMR; Q54Z27; -.
DR STRING; 44689.Q54Z27; -.
DR PaxDb; 44689-DDB0237577; -.
DR EnsemblProtists; EAL68145; EAL68145; DDB_G0277945.
DR GeneID; 8621236; -.
DR KEGG; ddi:DDB_G0277945; -.
DR dictyBase; DDB_G0277945; nol9.
DR eggNOG; KOG2750; Eukaryota.
DR HOGENOM; CLU_021128_2_0_1; -.
DR InParanoid; Q54Z27; -.
DR OMA; DTSPKNN; -.
DR PhylomeDB; Q54Z27; -.
DR Reactome; R-DDI-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR PRO; PR:Q54Z27; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046404; F:ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; ISS:UniProtKB.
DR GO; GO:0051731; F:polynucleotide 5'-hydroxyl-kinase activity; ISS:UniProtKB.
DR GO; GO:0000448; P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0000460; P:maturation of 5.8S rRNA; ISS:UniProtKB.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0006364; P:rRNA processing; ISS:UniProtKB.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR045116; Clp1/Grc3.
DR InterPro; IPR032319; CLP1_P.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR12755; CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P; 1.
DR PANTHER; PTHR12755:SF3; POLYNUCLEOTIDE 5'-HYDROXYL-KINASE NOL9; 1.
DR Pfam; PF16575; CLP1_P; 1.
PE 3: Inferred from homology;
KW ATP-binding; Coiled coil; Kinase; Nucleotide-binding; Nucleus;
KW Reference proteome; rRNA processing; Transferase.
FT CHAIN 1..683
FT /note="Polynucleotide 5'-hydroxyl-kinase nol9"
FT /id="PRO_0000368209"
FT REGION 1..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 414..442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 420..454
FT /evidence="ECO:0000255"
FT BINDING 277..284
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 683 AA; 78065 MW; 0D6F496DC86E827A CRC64;
MEINKNDNNN NINNNNINNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN QNNNQKNNYR
NYKPIKTGTL IQHQILQQTL SNFQTKNKVF QLGSDKLILI FKENETIYFH GTIQARSIIG
SVEVYGYTIT PQSTTYYPIY SPFCSPTLSI TSNNSNNKLY TLKEIIENQL IKIPELVKNK
LKENEKQIEI DEPTLAISSI IVLKILDDHC ENPKMFTKKK IEIIDSFKLL KGFYPLYQPD
FNTQLLTIPN EWLNLINTQF LYNDHCNNNG LSILTCGERN VGKSTFNRIL INKLLKKYTH
IIFIETDTGQ TEFTPSGIMS IDIINSPLLG PPFTHCKNNP LKSYFFGDTS PKNNPEYFLN
LSFQLIDCCN LIKQQYPNIP IILNTNGWLK SLGLHLTQEI IKYFKPTSIV QLINNNNNNN
NNNNNNNNNN NNNNNNNNNL NDNLNNFINR FDSTVTFSQD CINQLFEDCD DDNDDNKNLK
TTIITKLYSI NSINNFSQSI YKPIFNLGSS EIRNLMFKSY FKLESKGSLS HQSPYQVSWK
ELKVKILTNN VPPSQTMYAL NASLVGICID EDPEKNYKYI SYKVGNNLPT IINQSPLISQ
CIGLGLIRSI DMVNGYYYII TPIDPSLLEI SNTILKGSIE IPQTLTISVS SKGKILVPYL
QLDVLSSYGT GSDVMNRPSF FDD
//
