ID NOT3_CANAL Reviewed; 752 AA.
AC Q5AD56; A0A1D8PG97; Q5ADI5;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2017, sequence version 2.
DT 24-JAN-2024, entry version 84.
DE RecName: Full=General negative regulator of transcription subunit 3;
GN Name=NOT3; Synonyms=NOT31; OrderedLocusNames=CAALFM_C201130WA;
GN ORFNames=CaO19.2012, CaO19.9563;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=14500538; DOI=10.1128/iai.71.10.6101-6103.2003;
RA Cheng S., Nguyen M.H., Zhang Z., Jia H., Handfield M., Clancy C.J.;
RT "Evaluation of the roles of four Candida albicans genes in virulence by
RT using gene disruption strains that express URA3 from the native locus.";
RL Infect. Immun. 71:6101-6103(2003).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22359502; DOI=10.1371/journal.ppat.1002525;
RA Finkel J.S., Xu W., Huang D., Hill E.M., Desai J.V., Woolford C.A.,
RA Nett J.E., Taff H., Norice C.T., Andes D.R., Lanni F., Mitchell A.P.;
RT "Portrait of Candida albicans adherence regulators.";
RL PLoS Pathog. 8:E1002525-E1002525(2012).
CC -!- FUNCTION: Acts as a component of the CCR4-NOT core complex, which in
CC the nucleus seems to be a general transcription factor, and in the
CC cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT
CC protein subcomplex negatively regulates the basal and activated
CC transcription of many genes. Preferentially affects TC-type TATA
CC element-dependent transcription. Could directly or indirectly inhibit
CC component(s) of the general transcription machinery (By similarity).
CC Required for yeast cell adherence to silicone substrate, but nor
CC required not required for buccal epithelial cell adherence or virulence
CC in mouse systemic infection. {ECO:0000250, ECO:0000269|PubMed:14500538,
CC ECO:0000269|PubMed:22359502}.
CC -!- SUBUNIT: Component of the NOT protein complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Decreases cell adherence to silicone substrate.
CC Decreases slightly filamentation. {ECO:0000269|PubMed:14500538,
CC ECO:0000269|PubMed:22359502}.
CC -!- SIMILARITY: Belongs to the CNOT2/3/5 family. {ECO:0000305}.
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DR EMBL; CP017624; AOW27161.1; -; Genomic_DNA.
DR RefSeq; XP_019330745.1; XM_019475200.1.
DR AlphaFoldDB; Q5AD56; -.
DR SMR; Q5AD56; -.
DR STRING; 237561.Q5AD56; -.
DR EnsemblFungi; C2_01130W_A-T; C2_01130W_A-T-p1; C2_01130W_A.
DR GeneID; 3638829; -.
DR KEGG; cal:CAALFM_C201130WA; -.
DR CGD; CAL0000179957; NOT3.
DR VEuPathDB; FungiDB:C2_01130W_A; -.
DR eggNOG; KOG2150; Eukaryota.
DR HOGENOM; CLU_020483_0_0_1; -.
DR InParanoid; Q5AD56; -.
DR OrthoDB; 10289at2759; -.
DR PRO; PR:Q5AD56; -.
DR Proteomes; UP000000559; Chromosome 2.
DR GO; GO:0030015; C:CCR4-NOT core complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000932; C:P-body; IBA:GO_Central.
DR GO; GO:0030447; P:filamentous growth; IMP:CGD.
DR GO; GO:0044182; P:filamentous growth of a population of unicellular organisms; IMP:CGD.
DR GO; GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IBA:GO_Central.
DR GO; GO:1900189; P:positive regulation of cell adhesion involved in single-species biofilm formation; IMP:CGD.
DR GO; GO:0010811; P:positive regulation of cell-substrate adhesion; IMP:CGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:CGD.
DR GO; GO:0044011; P:single-species biofilm formation on inanimate substrate; IMP:CGD.
DR InterPro; IPR040168; Not2/3/5.
DR InterPro; IPR007207; Not_N.
DR PANTHER; PTHR23326; CCR4 NOT-RELATED; 1.
DR PANTHER; PTHR23326:SF1; CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 3; 1.
DR Pfam; PF04065; Not3; 1.
PE 3: Inferred from homology;
KW Activator; Coiled coil; Cytoplasm; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..752
FT /note="General negative regulator of transcription subunit
FT 3"
FT /id="PRO_0000426088"
FT REGION 126..160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 289..394
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 459..478
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 173..230
FT /evidence="ECO:0000255"
FT COMPBIAS 130..160
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 289..306
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..337
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 347..394
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 752 AA; 85707 MW; 7BF3D442F20C4D6F CRC64;
MSNRKLQKEI DIIFKKIQEG LQDFDYHYER YESIQNTEDD SDNQREKEKL ANDLKKEIKK
LQKFREQIKH WLQNDTVHTL GPVGTSYESK LAENKSLIED SMETYKLVEK QSKLKTFSNQ
SIMMTFMDST NGDDDDDDDD SDDDELFSGS DEDEEDEYSD LPEDAVNAIH FFKDSILQLR
EQTHKLDHEY EKLAAKKLRK NNLATIEAKK EKIQSTIGNN KFHQKKLKKL LRQLTSGVVT
EFNLIWLLKD DLNKYLESNG DYEFTRETEL YDDIFNQIAA VEEDYSEIHE DNHDHSHSHL
EKTSTANGTH HESATTTATA AKNSSVTTRT SPSPILTKAN GHIKELSVES GSPTTSRSKL
SKIHTNTSQS STHSNTSSST HAQAPRQSPE LTSPAIVKTL RPATAPSKPV GTLKWSAAAA
AAIPDVQEKP ELVETESNGS SVHKTVSKDQ YEEKINYQSP APTQQQQQPQ TSVESKTLST
TQSLASSITD NINLDKYKEV IKNSNLTKTE LGLFSDMNLA RVPPGIQDLV ISFASKRNND
NFKVLVDSTE FNQFALPLHK PYLPEIVQPS YYSQYTSTSF KHPTPLVKFQ SYWNQVRANF
GFQKLMDEIK SLTLQNNADN LPIIAELTFV LFYGFYYGVT PAENLIAESY LFELGWKPYK
NQSEDGSNNL STGSISLSDI NGSKSKSNRS SNYYFWFKRV KLISRGEDMG QPSNIEFGDY
QVFDLSFWEI FVKYGFKFDY NFCQMEPSKT LF
//
