ID NU50B_ARATH Reviewed; 465 AA.
AC Q9LW88; Q8GXC8; Q8LG62;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 24-JAN-2024, entry version 137.
DE RecName: Full=Nuclear pore complex protein NUP50B {ECO:0000303|PubMed:21189294};
DE AltName: Full=Nucleoporin 50B;
GN Name=NUP50B {ECO:0000303|PubMed:21189294};
GN OrderedLocusNames=At3g15970 {ECO:0000312|Araport:AT3G15970};
GN ORFNames=MSL1.1 {ECO:0000312|EMBL:BAB02663.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 392-465.
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP IDENTIFICATION IN THE NUCLEAR PORE COMPLEX, SUBCELLULAR LOCATION, FUNCTION,
RP AND NOMENCLATURE.
RX PubMed=21189294; DOI=10.1105/tpc.110.079947;
RA Tamura K., Fukao Y., Iwamoto M., Haraguchi T., Hara-Nishimura I.;
RT "Identification and characterization of nuclear pore complex components in
RT Arabidopsis thaliana.";
RL Plant Cell 22:4084-4097(2010).
CC -!- FUNCTION: Probably involved in nucleocytoplasmic transport via its
CC interactions with importins and Ran, rather than by forming part of the
CC nuclear pore complex (NPC) scaffolding. {ECO:0000305|PubMed:21189294}.
CC -!- SUBUNIT: Part of the nuclear pore complex (NPC). The NPC has an eight-
CC fold symmetrical structure comprising a central transport channel and
CC two rings, the cytoplasmic and nuclear rings, to which eight filaments
CC are attached. The cytoplasmic filaments have loose ends, while the
CC nuclear filaments are joined in a distal ring, forming a nuclear
CC basket. NPCs are highly dynamic in configuration and composition, and
CC can be devided in 3 subcomplexes, the NUP62 subcomplex, the NUP107-160
CC subcomplex and the NUP93 subcomplex, containing approximately 30
CC different nucleoporin proteins. {ECO:0000305|PubMed:21189294}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:21189294}. Nucleus, nuclear pore complex
CC {ECO:0000305|PubMed:21189294}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC42920.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB012247; BAB02663.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75754.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64912.1; -; Genomic_DNA.
DR EMBL; AY084443; AAM61016.1; -; mRNA.
DR EMBL; AK118302; BAC42920.1; ALT_INIT; mRNA.
DR RefSeq; NP_001326913.1; NM_001338194.1.
DR RefSeq; NP_566532.1; NM_112467.3.
DR AlphaFoldDB; Q9LW88; -.
DR SMR; Q9LW88; -.
DR BioGRID; 6175; 2.
DR IntAct; Q9LW88; 1.
DR STRING; 3702.Q9LW88; -.
DR iPTMnet; Q9LW88; -.
DR PaxDb; 3702-AT3G15970-1; -.
DR ProteomicsDB; 248662; -.
DR EnsemblPlants; AT3G15970.1; AT3G15970.1; AT3G15970.
DR EnsemblPlants; AT3G15970.2; AT3G15970.2; AT3G15970.
DR GeneID; 820841; -.
DR Gramene; AT3G15970.1; AT3G15970.1; AT3G15970.
DR Gramene; AT3G15970.2; AT3G15970.2; AT3G15970.
DR KEGG; ath:AT3G15970; -.
DR Araport; AT3G15970; -.
DR TAIR; AT3G15970; -.
DR eggNOG; KOG0864; Eukaryota.
DR HOGENOM; CLU_050764_1_0_1; -.
DR InParanoid; Q9LW88; -.
DR OMA; VMFEYLE; -.
DR OrthoDB; 307490at2759; -.
DR PhylomeDB; Q9LW88; -.
DR PRO; PR:Q9LW88; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LW88; baseline and differential.
DR Genevisible; Q9LW88; AT.
DR GO; GO:0005643; C:nuclear pore; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046907; P:intracellular transport; IEA:InterPro.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR CDD; cd13169; RanBD_NUP50_plant; 1.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR InterPro; IPR015007; NUP2/50/61.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR000156; Ran_bind_dom.
DR InterPro; IPR045207; RanBD_NUP50_plant.
DR InterPro; IPR045255; RanBP1-like.
DR PANTHER; PTHR23138:SF142; NUCLEAR PORE COMPLEX PROTEIN NUP50A-RELATED; 1.
DR PANTHER; PTHR23138; RAN BINDING PROTEIN; 1.
DR Pfam; PF08911; NUP50; 1.
DR Pfam; PF00638; Ran_BP1; 1.
DR SMART; SM00160; RanBD; 1.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR PROSITE; PS50196; RANBD1; 1.
PE 1: Evidence at protein level;
KW Acetylation; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein;
KW Protein transport; Reference proteome; Repeat; Translocation; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9C829"
FT CHAIN 2..465
FT /note="Nuclear pore complex protein NUP50B"
FT /id="PRO_0000431081"
FT REPEAT 266..267
FT /note="1"
FT REPEAT 286..287
FT /note="2"
FT REPEAT 297..298
FT /note="3"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 59..244
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 266..298
FT /note="3 X 2 AA repeats of F-G"
FT REGION 308..330
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 439..465
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 108..130
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 139..154
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 191..211
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 212..231
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 308..323
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylglycine"
FT /evidence="ECO:0000250|UniProtKB:Q9C829"
FT MOD_RES 125
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9C829"
FT MOD_RES 455
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9C829"
FT MOD_RES 459
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9C829"
FT CONFLICT 223
FT /note="G -> C (in Ref. 3; AAM61016)"
FT CONFLICT 382
FT /note="T -> I (in Ref. 3; AAM61016)"
SQ SEQUENCE 465 AA; 49108 MW; FC3CA51234EE9CAC CRC64;
MGDSDNAIPF SRKRTALKEL SRDNPGLDDD DEDTSALESG TFNTASKEVL ASRRIIRVRR
TDRSATAPPA SNPFTGIRLV PFTAPAPSTA AAETTKPLSA GKQETLADGR SDATKETDGD
SKEKSDAIDA VGKQETQGDE ISAKTKDIID GGEKEMSEAV NSVEGGGAVN KNEDEIKTTM
VTEVAAGEET VKDDNNNSNT VEGSDCVVKD TGGNQTEKEG KEGDGNEDTE KNGDSGALSS
FHQHSSSKNA FTGLASTGFS ASSFSFGLVP QEGSTGSGSE QSSFSFGQAN NGNSSLFGAS
VATSITTKST ETTTAFPSKQ DVSVETGEEN EKAAFTADSV MFEYLEGGWK ERGKGELKVN
ISTTENRKAR LVMRSKGNYR LTLNASLYPE MKLAKMDKKG ITFACVNSVS DAKDGLSTLA
LKFKDPTVVE EFRAVIEEHK DSKPSVAEAA APLKTPENSP SAEDA
//
