GenomeNet

Database: UniProt
Entry: O23034
LinkDB: O23034
Original site: O23034 
ID   UBA5_ARATH              Reviewed;         431 AA.
AC   O23034;
DT   02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   02-MAR-2010, sequence version 2.
DT   13-FEB-2019, entry version 123.
DE   RecName: Full=Ubiquitin-like modifier-activating enzyme 5;
DE            Short=Ubiquitin-activating enzyme 5;
GN   OrderedLocusNames=At1g05350; ORFNames=YUP8H12.3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA   White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA   Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA   Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA   Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA   Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA   Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA   Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA   Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA   Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA   Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA   Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA   Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA   Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA   Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana
RT   reference genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- FUNCTION: E1-like enzyme which activates UFM1. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-activating E1 family. UBA5
CC       subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB71466.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AC000098; AAB71466.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE27828.1; -; Genomic_DNA.
DR   PIR; D86188; D86188.
DR   RefSeq; NP_172027.2; NM_100414.4.
DR   UniGene; At.51488; -.
DR   ProteinModelPortal; O23034; -.
DR   SMR; O23034; -.
DR   STRING; 3702.AT1G05350.1; -.
DR   iPTMnet; O23034; -.
DR   PaxDb; O23034; -.
DR   PRIDE; O23034; -.
DR   EnsemblPlants; AT1G05350.1; AT1G05350.1; AT1G05350.
DR   GeneID; 837034; -.
DR   Gramene; AT1G05350.1; AT1G05350.1; AT1G05350.
DR   KEGG; ath:AT1G05350; -.
DR   Araport; AT1G05350; -.
DR   TAIR; locus:2207185; AT1G05350.
DR   eggNOG; KOG2336; Eukaryota.
DR   eggNOG; COG0476; LUCA.
DR   HOGENOM; HOG000256352; -.
DR   InParanoid; O23034; -.
DR   KO; K12164; -.
DR   OMA; ETHNYNI; -.
DR   OrthoDB; 1092362at2759; -.
DR   PhylomeDB; O23034; -.
DR   Reactome; R-ATH-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   PRO; PR:O23034; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   Genevisible; O23034; AT.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; IDA:TAIR.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0071566; F:UFM1 activating enzyme activity; IBA:GO_Central.
DR   GO; GO:0032446; P:protein modification by small protein conjugation; IBA:GO_Central.
DR   GO; GO:0071569; P:protein ufmylation; IBA:GO_Central.
DR   InterPro; IPR029752; D-isomer_DH_CS1.
DR   InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR   InterPro; IPR035985; Ubiquitin-activating_enz.
DR   Pfam; PF00899; ThiF; 1.
DR   SUPFAM; SSF69572; SSF69572; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Metal-binding; Nucleotide-binding;
KW   Reference proteome; Ubl conjugation pathway; Zinc.
FT   CHAIN         1    431       Ubiquitin-like modifier-activating enzyme
FT                                5.
FT                                /FTId=PRO_0000391953.
FT   ACT_SITE    263    263       Glycyl thioester intermediate.
FT                                {ECO:0000250}.
FT   METAL       239    239       Zinc. {ECO:0000250}.
FT   METAL       242    242       Zinc. {ECO:0000250}.
FT   METAL       316    316       Zinc. {ECO:0000250}.
FT   METAL       321    321       Zinc. {ECO:0000250}.
FT   BINDING      92     92       ATP; via amide nitrogen. {ECO:0000250}.
FT   BINDING     113    113       ATP. {ECO:0000250}.
FT   BINDING     136    136       ATP. {ECO:0000250}.
FT   BINDING     159    159       ATP. {ECO:0000250}.
FT   BINDING     197    197       ATP. {ECO:0000250}.
SQ   SEQUENCE   431 AA;  47020 MW;  4809072C8873287F CRC64;
     MEVGFKALLD DLDVLEKSLS DPALINKLRS HVENLATLSK CNPHRRSKVK ELSSEVVDSN
     PYSRLMALQR MGIVDNYERI REFSVAIVGI GGVGSVAAEM LTRCGIGRLL LYDYDTVELA
     NMNRLFFRPD QVGMTKTDAA VQTLAEINPD VVLESFTMNI TTVQGFETFT SSLTNKSFCP
     SKEGGSGVDL VLSCVDNYEA RMAVNQACNE LRQTWMESGV SEDAVSGHIQ LLVPGETACF
     ACAPPLVVAS GIDERTLKRE GVCAASLPTT MGVVAGLLVQ NSLKFLLNFG EVSPYLGYNS
     LKDFFPTMKM RPNPQCSNVA CLERQKEYML AKPERDAAAK AKMEADASTT IDEGPLHDDN
     EWNISVVDDE NEKDTTKAAS SSDTLPEGLT RELPVADEYE KAIAIASGSG ETEEEDDLED
     LKKQLEALNA A
//
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