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Database: UniProt
Entry: O25029
LinkDB: O25029
Original site: O25029 
ID   RHPA_HELPY              Reviewed;         492 AA.
AC   O25029;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   11-DEC-2019, entry version 119.
DE   RecName: Full=DEAD-box ATP-dependent RNA helicase RhpA;
DE            EC=3.6.4.13;
GN   Name=rhpA; OrderedLocusNames=HP_0247, C694_01250;
OS   Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700392 / 26695;
RX   PubMed=9252185; DOI=10.1038/41483;
RA   Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA   Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA   Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA   Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA   McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA   Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA   Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA   Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT   "The complete genome sequence of the gastric pathogen Helicobacter
RT   pylori.";
RL   Nature 388:539-547(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700392 / 26695;
RA   Manolov A., Prihodko E., Larin A., Karpova I., Semashko T., Alexeev D.,
RA   Kostrjukova E., Govorun V.;
RT   "Draft genome of Helicobacter pylori.";
RL   Submitted (OCT-2012) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   INTERACTION WITH RNJ, AND SUBUNIT.
RC   STRAIN=ATCC 700392 / 26695;
RX   PubMed=23093592; DOI=10.1093/nar/gks945;
RA   Redko Y., Aubert S., Stachowicz A., Lenormand P., Namane A., Darfeuille F.,
RA   Thibonnier M., De Reuse H.;
RT   "A minimal bacterial RNase J-based degradosome is associated with
RT   translating ribosomes.";
RL   Nucleic Acids Res. 41:288-301(2013).
CC   -!- FUNCTION: DEAD-box RNA helicase probably involved in RNA degradation.
CC       Unwinds dsRNA in both 5'- and 3'-directions (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Homodimer (By similarity). Interacts with RNase J (rnj), might
CC       be a member of a minimal RNA degradosome complex. {ECO:0000250,
CC       ECO:0000269|PubMed:23093592}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Note=The RNaseJ-RhpA complex co-
CC       localizes with 70S ribosomes and polysomes; remains associated with
CC       ribosomes in the absence of RNase J. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. {ECO:0000305}.
DR   EMBL; AE000511; AAD07315.1; -; Genomic_DNA.
DR   EMBL; CP003904; AFV41471.1; -; Genomic_DNA.
DR   PIR; G64550; G64550.
DR   RefSeq; NP_207045.1; NC_000915.1.
DR   RefSeq; WP_000422563.1; NC_018939.1.
DR   SMR; O25029; -.
DR   DIP; DIP-3203N; -.
DR   IntAct; O25029; 7.
DR   MINT; O25029; -.
DR   STRING; 85962.C694_01250; -.
DR   PaxDb; O25029; -.
DR   EnsemblBacteria; AAD07315; AAD07315; HP_0247.
DR   EnsemblBacteria; AFV41471; AFV41471; C694_01250.
DR   GeneID; 900197; -.
DR   KEGG; heo:C694_01250; -.
DR   KEGG; hpy:HP0247; -.
DR   PATRIC; fig|85962.47.peg.267; -.
DR   eggNOG; ENOG4105C1J; Bacteria.
DR   eggNOG; COG0513; LUCA.
DR   KO; K05592; -.
DR   OMA; TEHPIDQ; -.
DR   BioCyc; HPY:HP0247-MONOMER; -.
DR   Proteomes; UP000000429; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0033592; F:RNA strand annealing activity; IBA:GO_Central.
DR   GO; GO:0000027; P:ribosomal large subunit assembly; IBA:GO_Central.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cytoplasm; Helicase; Hydrolase; Nucleotide-binding;
KW   Reference proteome; RNA-binding.
FT   CHAIN           1..492
FT                   /note="DEAD-box ATP-dependent RNA helicase RhpA"
FT                   /id="PRO_0000430105"
FT   DOMAIN          51..220
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          231..393
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   NP_BIND         64..71
FT                   /note="ATP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   MOTIF           20..48
FT                   /note="Q motif"
FT   MOTIF           168..171
FT                   /note="DEAD box"
SQ   SEQUENCE   492 AA;  55806 MW;  C5E35D6C73BB21ED CRC64;
     MELNQPPLPT EIDGDAYHKP SFNDLGLKES VLKSVYEAGF TSPSPIQEKA IPAVLQGRDV
     IAQAQTGTGK TAAFALPIIN NLKNNHTIEA LVITPTRELA MQISDEIFKL GKHTRTKTVC
     VYGGQSVKKQ CEFIKKNPQV MIATPGRLLD HLKNERIHKF VPKVVVLDES DEMLDMGFLD
     DIEEIFDYLP SEAQILLFSA TMPEPIKRLA DKILENPIKI HIAPSNITNT DITQRFYVIN
     EHERAEAIMR LLDTQAPKKS IVFTRTKKEA DELHQFLASK NYKSTALHGD MDQRDRRSSI
     MAFKKNDADV LVATDVASRG LDISGVSHVF NYHLPLNTES YIHRIGRTGR AGKKGMAITL
     VTPLEYKELL RMQKEIDSEI ELFEIPTINE NQIIKTLHDA KVSEGIISLY EQLTEIFEPS
     QLVLKLLSLQ FETSKIGLNQ QEIDAIQNPK EKTPKPSNKK TPQHERARSF KKGQHRDRHP
     KTNHYSKKPK RR
//
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