ID ECFA_METTH Reviewed; 311 AA.
AC O27739;
DT 21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 27-MAR-2024, entry version 142.
DE RecName: Full=Energy-coupling factor transporter ATP-binding protein EcfA {ECO:0000255|HAMAP-Rule:MF_01710};
DE Short=ECF transporter A component EcfA {ECO:0000255|HAMAP-Rule:MF_01710};
DE EC=3.6.3.- {ECO:0000255|HAMAP-Rule:MF_01710};
GN Name=ecfA {ECO:0000255|HAMAP-Rule:MF_01710}; Synonyms=cbiO;
GN OrderedLocusNames=MTH_1704;
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
CC -!- FUNCTION: ATP-binding (A) component of a common energy-coupling factor
CC (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF
CC transporter provides the energy necessary to transport a number of
CC different substrates. {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC complex composed of 2 membrane-embedded substrate-binding proteins (S
CC component), 2 ATP-binding proteins (A component) and 2 transmembrane
CC proteins (T component). {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01710};
CC Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Energy-coupling
CC factor EcfA family. {ECO:0000255|HAMAP-Rule:MF_01710}.
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DR EMBL; AE000666; AAB86176.1; -; Genomic_DNA.
DR PIR; A69095; A69095.
DR RefSeq; WP_010877312.1; NC_000916.1.
DR AlphaFoldDB; O27739; -.
DR SMR; O27739; -.
DR STRING; 187420.MTH_1704; -.
DR PaxDb; 187420-MTH_1704; -.
DR EnsemblBacteria; AAB86176; AAB86176; MTH_1704.
DR GeneID; 77402222; -.
DR KEGG; mth:MTH_1704; -.
DR PATRIC; fig|187420.15.peg.1665; -.
DR HOGENOM; CLU_000604_1_22_2; -.
DR InParanoid; O27739; -.
DR Proteomes; UP000005223; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0006824; P:cobalt ion transport; IEA:InterPro.
DR CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR InterPro; IPR005876; Co_trans_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR01166; cbiO; 1.
DR PANTHER; PTHR43553:SF28; ENERGY-COUPLING FACTOR TRANSPORTER ATP-BINDING PROTEIN ECFA; 1.
DR PANTHER; PTHR43553; HEAVY METAL TRANSPORTER; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51246; CBIO; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell membrane; Hydrolase; Membrane; Nucleotide-binding;
KW Reference proteome; Transport.
FT CHAIN 1..311
FT /note="Energy-coupling factor transporter ATP-binding
FT protein EcfA"
FT /id="PRO_0000092153"
FT DOMAIN 2..237
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT BINDING 35..42
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
SQ SEQUENCE 311 AA; 34786 MW; 1577855C7D1DA097 CRC64;
MIELRDLSYS YPDGTPALRG INMKVERGER VAVIGPNGAG KSTLFLHLNG ILKPAAGEVI
IDGERVDYSK DELIKIRQKV GIVFQNPDDQ LFSPTVREDV AFGPMNLGLP EDEVEERVAE
SLEKVGMSGY ENRAPHHLSG GEKKRVAIAG ILAMKPEIMV LDEPTTGLDP ETADGIIRIL
LELSREGITV MISSHDVEII SQFAERVFVL NSGELIAEGT PLEIFRDAEL IRRASLRLPR
TADLLNRLRM AGFEVDVKLT VEETYHELLH LLGGDAYHRL LHFLGEEKQH RLIHLLGEKK
YHELLHALKE Q
//
