ID LMRB_BACSU Reviewed; 479 AA.
AC O35018;
DT 16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 27-MAR-2024, entry version 131.
DE RecName: Full=Lincomycin resistance protein LmrB;
GN Name=lmrB; Synonyms=yccA; OrderedLocusNames=BSU02670;
OS Bacillus subtilis (strain 168).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9274031; DOI=10.1099/00221287-143-8-2775;
RA Kumano M., Tamakoshi A., Yamane K.;
RT "A 32 kb nucleotide sequence from the region of the lincomycin-resistance
RT gene (22 degrees-25 degrees) of the Bacillus subtilis chromosome and
RT identification of the site of the lin-2 mutation.";
RL Microbiology 143:2775-2782(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
CC -!- FUNCTION: Proton-dependent transporter. May mediate the efflux of
CC lincomycin.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. EmrB family.
CC {ECO:0000305}.
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DR EMBL; AB000617; BAA22228.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12061.1; -; Genomic_DNA.
DR PIR; E69652; E69652.
DR RefSeq; NP_388149.1; NC_000964.3.
DR RefSeq; WP_010886396.1; NZ_JNCM01000030.1.
DR AlphaFoldDB; O35018; -.
DR SMR; O35018; -.
DR STRING; 224308.BSU02670; -.
DR TCDB; 2.A.1.3.30; the major facilitator superfamily (mfs).
DR PaxDb; 224308-BSU02670; -.
DR EnsemblBacteria; CAB12061; CAB12061; BSU_02670.
DR GeneID; 938388; -.
DR KEGG; bsu:BSU02670; -.
DR PATRIC; fig|224308.179.peg.277; -.
DR eggNOG; COG0477; Bacteria.
DR InParanoid; O35018; -.
DR OrthoDB; 9816041at2; -.
DR PhylomeDB; O35018; -.
DR BioCyc; BSUB:BSU02670-MONOMER; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR CDD; cd17503; MFS_LmrB_MDR_like; 1.
DR Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 1.
DR Gene3D; 1.20.1720.10; Multidrug resistance protein D; 1.
DR InterPro; IPR004638; EmrB-like.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR NCBIfam; TIGR00711; efflux_EmrB; 1.
DR PANTHER; PTHR42718:SF43; LINCOMYCIN RESISTANCE PROTEIN LMRB; 1.
DR PANTHER; PTHR42718; MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC; 1.
DR Pfam; PF07690; MFS_1; 1.
DR PRINTS; PR01036; TCRTETB.
DR SUPFAM; SSF103473; MFS general substrate transporter; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..479
FT /note="Lincomycin resistance protein LmrB"
FT /id="PRO_0000173328"
FT TRANSMEM 19..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..251
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..355
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 360..382
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 403..425
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 449..471
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 479 AA; 51709 MW; 0905DA76EF3A053D CRC64;
MILETTAKAS QQYKVMPIMI SLLLAGFIGM FSETALNIAL TDLMKELNIT AATVQWLTTG
YLLVLGILVP VSGLLLQWFT TRQLFTVSLI FSILGTFIAA LAPSFSFLLA ARIVQALGTG
LLLPLMFNTI LVIFPPHKRG AAMGTIGLVI MFAPAIGPTF SGLVLEHLNW HWIFWISLPF
LVLALVFGIA YMQNVSETTK PKIDVLSIIL STIGFGGIVF GFSNAGEGSG GWSSPTVIVS
LIVGVVGLIL FSIRQLTMKQ PMMNLRAFKY PMFILGVIMV FICMMVILSS MLLLPMYLQG
GLVLTAFASG LVLLPGGILN GFMSPVTGRL FDKYGPKWLV IPGFVIVTVV LWFFSNVTTT
STAVLIIILH TCLMIGISMI MMPAQTNGLN QLPREFYPDG TAIMNTLQQM AGAIGTAVAV
SIMAAGQHDY MSTVKNPADP AVIPQALTAG VQHAFVFAMI VAIIGLIGAF FMKRVKVDH
//
