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Database: UniProt
Entry: O54775
LinkDB: O54775
Original site: O54775 
ID   WISP1_MOUSE             Reviewed;         367 AA.
AC   O54775; Q80ZL1;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   05-JUN-2019, entry version 147.
DE   RecName: Full=WNT1-inducible-signaling pathway protein 1;
DE            Short=WISP-1;
DE   AltName: Full=CCN family member 4;
DE   AltName: Full=ELM-1;
DE   Flags: Precursor;
GN   Name=Wisp1; Synonyms=Ccn4, Elm1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=HeN;
RX   PubMed=9449709; DOI=10.1084/jem.187.3.289;
RA   Hashimoto Y., Shindo-Okada N., Tani M., Nagamachi Y., Takeuchi K.,
RA   Shiroishi T., Toma H., Yokota J.;
RT   "Expression of the Elm1 gene, a novel gene of the CCN (connective
RT   tissue growth factor, Cyr61/Cef10, and neuroblastoma overexpressed
RT   gene) family, suppresses In vivo tumor growth and metastasis of K-1735
RT   murine melanoma cells.";
RL   J. Exp. Med. 187:289-296(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Mammary gland;
RX   PubMed=9843955; DOI=10.1073/pnas.95.25.14717;
RA   Pennica D., Swanson T.A., Welsh J.W., Roy M.A., Lawrence D.A., Lee J.,
RA   Brush J., Taneyhill L.A., Deuel B., Lew M., Watanabe C., Cohen R.L.,
RA   Melham M.F., Finley G.G., Quirke P., Goddard A.D., Hillan K.J.,
RA   Gurney A.L., Botstein D., Levine A.J.;
RT   "WISP genes are members of the connective tissue growth factor family
RT   that are up-regulated in wnt-1-transformed cells and aberrantly
RT   expressed in human colon tumors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 95:14717-14722(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C3H/HeN, and FVB/N; TISSUE=Colon, and Mesenchymal cell;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Downstream regulator in the Wnt/Frizzled-signaling
CC       pathway (By similarity). Associated with cell survival. Adheres to
CC       skin and melanoma fibroblasts (By similarity). In vitro binding to
CC       skin fibroblasts occurs through the proteoglycans, decorin and
CC       biglycan (By similarity). Suppresses tumor growth in vivo.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in kidney and lung. Lower
CC       levels in heart, brain, spleen, liver, skeletal muscle and testis.
CC       Expressed in low metastatic melanoma cells.
CC   -!- SIMILARITY: Belongs to the CCN family. {ECO:0000305}.
DR   EMBL; AB004873; BAA24949.1; -; mRNA.
DR   EMBL; AF100777; AAC96319.1; -; mRNA.
DR   EMBL; BC048791; AAH48791.1; -; mRNA.
DR   EMBL; BC052677; AAH52677.1; -; mRNA.
DR   CCDS; CCDS27510.1; -.
DR   RefSeq; NP_061353.1; NM_018865.2.
DR   SMR; O54775; -.
DR   STRING; 10090.ENSMUSP00000005255; -.
DR   PhosphoSitePlus; O54775; -.
DR   PaxDb; O54775; -.
DR   PeptideAtlas; O54775; -.
DR   PRIDE; O54775; -.
DR   Ensembl; ENSMUST00000005255; ENSMUSP00000005255; ENSMUSG00000005124.
DR   GeneID; 22402; -.
DR   KEGG; mmu:22402; -.
DR   UCSC; uc007wau.1; mouse.
DR   CTD; 8840; -.
DR   MGI; MGI:1197008; Wisp1.
DR   eggNOG; ENOG410IH8M; Eukaryota.
DR   eggNOG; ENOG410ZWR5; LUCA.
DR   GeneTree; ENSGT00940000158587; -.
DR   HOGENOM; HOG000231462; -.
DR   InParanoid; O54775; -.
DR   KO; K22471; -.
DR   OMA; GEVEPWH; -.
DR   OrthoDB; 601212at2759; -.
DR   PhylomeDB; O54775; -.
DR   TreeFam; TF326070; -.
DR   ChiTaRS; Wisp1; mouse.
DR   PRO; PR:O54775; -.
DR   Proteomes; UP000000589; Chromosome 15.
DR   Bgee; ENSMUSG00000005124; Expressed in 229 organ(s), highest expression level in molar tooth.
DR   ExpressionAtlas; O54775; baseline and differential.
DR   Genevisible; O54775; MM.
DR   GO; GO:0005737; C:cytoplasm; IMP:UniProtKB.
DR   GO; GO:0005829; C:cytosol; ISO:MGI.
DR   GO; GO:0031012; C:extracellular matrix; IBA:GO_Central.
DR   GO; GO:0005615; C:extracellular space; HDA:BHF-UCL.
DR   GO; GO:0008201; F:heparin binding; IBA:GO_Central.
DR   GO; GO:0005520; F:insulin-like growth factor binding; IEA:InterPro.
DR   GO; GO:0005178; F:integrin binding; IBA:GO_Central.
DR   GO; GO:0060348; P:bone development; IMP:UniProtKB.
DR   GO; GO:0007155; P:cell adhesion; IBA:GO_Central.
DR   GO; GO:0042593; P:glucose homeostasis; IMP:UniProtKB.
DR   GO; GO:0060548; P:negative regulation of cell death; IBA:GO_Central.
DR   GO; GO:0032331; P:negative regulation of chondrocyte differentiation; IMP:UniProtKB.
DR   GO; GO:0045599; P:negative regulation of fat cell differentiation; IDA:UniProtKB.
DR   GO; GO:0001649; P:osteoblast differentiation; IMP:UniProtKB.
DR   GO; GO:0030316; P:osteoclast differentiation; IMP:UniProtKB.
DR   GO; GO:0061051; P:positive regulation of cell growth involved in cardiac muscle cell development; ISO:MGI.
DR   GO; GO:0050729; P:positive regulation of inflammatory response; IEA:Ensembl.
DR   GO; GO:0045669; P:positive regulation of osteoblast differentiation; IEA:Ensembl.
DR   GO; GO:0014911; P:positive regulation of smooth muscle cell migration; ISS:UniProtKB.
DR   GO; GO:0048661; P:positive regulation of smooth muscle cell proliferation; ISS:UniProtKB.
DR   GO; GO:0030177; P:positive regulation of Wnt signaling pathway; IMP:UniProtKB.
DR   GO; GO:0090303; P:positive regulation of wound healing; IMP:UniProtKB.
DR   GO; GO:0050707; P:regulation of cytokine secretion; IEA:Ensembl.
DR   GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR   Gene3D; 2.20.100.10; -; 1.
DR   InterPro; IPR006207; Cys_knot_C.
DR   InterPro; IPR006208; Glyco_hormone_CN.
DR   InterPro; IPR009030; Growth_fac_rcpt_cys_sf.
DR   InterPro; IPR000867; IGFBP-like.
DR   InterPro; IPR012395; IGFBP_CNN.
DR   InterPro; IPR017891; Insulin_GF-bd_Cys-rich_CS.
DR   InterPro; IPR000884; TSP1_rpt.
DR   InterPro; IPR036383; TSP1_rpt_sf.
DR   InterPro; IPR001007; VWF_dom.
DR   Pfam; PF00007; Cys_knot; 1.
DR   Pfam; PF00219; IGFBP; 1.
DR   Pfam; PF00090; TSP_1; 1.
DR   Pfam; PF00093; VWC; 1.
DR   PIRSF; PIRSF036495; IGFBP_rP_CNN; 1.
DR   SMART; SM00041; CT; 1.
DR   SMART; SM00121; IB; 1.
DR   SMART; SM00209; TSP1; 1.
DR   SMART; SM00214; VWC; 1.
DR   SUPFAM; SSF57184; SSF57184; 1.
DR   SUPFAM; SSF82895; SSF82895; 1.
DR   PROSITE; PS01185; CTCK_1; 1.
DR   PROSITE; PS01225; CTCK_2; 1.
DR   PROSITE; PS00222; IGFBP_N_1; 1.
DR   PROSITE; PS51323; IGFBP_N_2; 1.
DR   PROSITE; PS50092; TSP1; 1.
DR   PROSITE; PS01208; VWFC_1; 1.
DR   PROSITE; PS50184; VWFC_2; 1.
PE   2: Evidence at transcript level;
KW   Cell adhesion; Complete proteome; Disulfide bond; Glycoprotein;
KW   Proto-oncogene; Reference proteome; Secreted; Signal;
KW   Wnt signaling pathway.
FT   SIGNAL        1     22       {ECO:0000255}.
FT   CHAIN        23    367       WNT1-inducible-signaling pathway protein
FT                                1.
FT                                /FTId=PRO_0000014407.
FT   DOMAIN       45    118       IGFBP N-terminal. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00653}.
FT   DOMAIN      121    186       VWFC. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00220}.
FT   DOMAIN      215    260       TSP type-1. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00210}.
FT   DOMAIN      273    347       CTCK. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00039}.
FT   CARBOHYD     86     86       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    143    143       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    284    284       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    343    343       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   DISULFID    273    310       {ECO:0000250}.
FT   DISULFID    290    324       {ECO:0000250}.
FT   DISULFID    301    340       {ECO:0000250}.
FT   DISULFID    304    342       {ECO:0000250}.
FT   DISULFID    309    346       {ECO:0000250}.
FT   CONFLICT     79     79       C -> R (in Ref. 3; AAH52677).
FT                                {ECO:0000305}.
FT   CONFLICT    199    199       D -> G (in Ref. 3; AAH52677).
FT                                {ECO:0000305}.
FT   CONFLICT    316    316       K -> R (in Ref. 3; AAH52677).
FT                                {ECO:0000305}.
FT   CONFLICT    363    363       E -> G (in Ref. 3; AAH52677).
FT                                {ECO:0000305}.
SQ   SEQUENCE   367 AA;  40703 MW;  3B7C0569EFAB5E96 CRC64;
     MRWLLPWTLA AVAVLRVGNI LATALSPTPT TMTFTPAPLE ETTTRPEFCK WPCECPQSPP
     RCPLGVSLIT DGCECCKICA QQLGDNCTEA AICDPHRGLY CDYSGDRPRY AIGVCAQVVG
     VGCVLDGVRY TNGESFQPNC RYNCTCIDGT VGCTPLCLSP RPPRLWCRQP RHVRVPGQCC
     EQWVCDDDAR RPRQTALLDT RAFAASGAVE QRYENCIAYT SPWSPCSTTC GLGISTRISN
     VNARCWPEQE SRLCNLRPCD VDIQLHIKAG KKCLAVYQPE EATNFTLAGC VSTRTYRPKY
     CGVCTDNRCC IPYKSKTISV DFQCPEGPGF SRQVLWINAC FCNLSCRNPN DIFADLESYP
     DFEEIAN
//
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