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Database: UniProt
Entry: O58320
LinkDB: O58320
Original site: O58320 
ID   GYAR_PYRHO              Reviewed;         334 AA.
AC   O58320;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2004, sequence version 2.
DT   16-JAN-2019, entry version 114.
DE   RecName: Full=Glyoxylate reductase;
DE            EC=1.1.1.26;
GN   Name=gyaR; OrderedLocusNames=PH0597;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 /
OS   NBRC 100139 / OT-3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y.,
RA   Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y.,
RA   Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y.,
RA   Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA   Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K.,
RA   Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH NADP.
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RG   RIKEN structural genomics initiative (RSGI);
RT   "Crystal structure of glyoxylate reductase (ph0597) from Pyrococcus
RT   horikoshii OT3, complexed with nadp (i41).";
RL   Submitted (JUN-2006) to the PDB data bank.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glycolate + NAD(+) = glyoxylate + H(+) + NADH;
CC         Xref=Rhea:RHEA:18229, ChEBI:CHEBI:15378, ChEBI:CHEBI:29805,
CC         ChEBI:CHEBI:36655, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC         EC=1.1.1.26;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid
CC       dehydrogenase family. GyaR subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA29686.1; Type=Erroneous initiation; Evidence={ECO:0000305};
DR   EMBL; BA000001; BAA29686.1; ALT_INIT; Genomic_DNA.
DR   PIR; A71175; A71175.
DR   RefSeq; WP_010884698.1; NC_000961.1.
DR   PDB; 2DBQ; X-ray; 1.70 A; A=1-334.
DR   PDB; 2DBR; X-ray; 2.61 A; A/B/C/D/E/F=1-334.
DR   PDB; 2DBZ; X-ray; 2.45 A; A/B=1-334.
DR   PDBsum; 2DBQ; -.
DR   PDBsum; 2DBR; -.
DR   PDBsum; 2DBZ; -.
DR   ProteinModelPortal; O58320; -.
DR   SMR; O58320; -.
DR   STRING; 70601.PH0597; -.
DR   PRIDE; O58320; -.
DR   EnsemblBacteria; BAA29686; BAA29686; BAA29686.
DR   GeneID; 1442932; -.
DR   KEGG; pho:PH0597; -.
DR   eggNOG; arCOG01755; Archaea.
DR   eggNOG; COG1052; LUCA.
DR   HOGENOM; HOG000136700; -.
DR   KO; K00015; -.
DR   OMA; KWIAHNG; -.
DR   OrthoDB; 36410at2157; -.
DR   BioCyc; PHOR70601:G1G39-586-MONOMER; -.
DR   EvolutionaryTrace; O58320; -.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0047964; F:glyoxylate reductase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   HAMAP; MF_00776; GyaR; 1.
DR   InterPro; IPR006139; D-isomer_2_OHA_DH_cat_dom.
DR   InterPro; IPR029753; D-isomer_DH_CS.
DR   InterPro; IPR029752; D-isomer_DH_CS1.
DR   InterPro; IPR006140; D-isomer_DH_NAD-bd.
DR   InterPro; IPR023519; Glyoxylate_reductase_GyaR.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF00389; 2-Hacid_dh; 1.
DR   Pfam; PF02826; 2-Hacid_dh_C; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00065; D_2_HYDROXYACID_DH_1; 1.
DR   PROSITE; PS00671; D_2_HYDROXYACID_DH_3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Cytoplasm; NAD; Oxidoreductase.
FT   CHAIN         1    334       Glyoxylate reductase.
FT                                /FTId=PRO_0000075949.
FT   NP_BIND     158    161       NADP. {ECO:0000269|Ref.2}.
FT   NP_BIND     180    182       NADP. {ECO:0000269|Ref.2}.
FT   NP_BIND     239    241       NADP. {ECO:0000269|Ref.2}.
FT   NP_BIND     288    290       NADP. {ECO:0000269|Ref.2}.
FT   ACT_SITE    241    241       {ECO:0000250}.
FT   ACT_SITE    270    270       {ECO:0000250}.
FT   ACT_SITE    288    288       Proton donor. {ECO:0000250}.
FT   STRAND        4      9       {ECO:0000244|PDB:2DBQ}.
FT   HELIX        13     20       {ECO:0000244|PDB:2DBQ}.
FT   STRAND       23     27       {ECO:0000244|PDB:2DBQ}.
FT   STRAND       30     32       {ECO:0000244|PDB:2DBZ}.
FT   HELIX        36     42       {ECO:0000244|PDB:2DBQ}.
FT   TURN         43     45       {ECO:0000244|PDB:2DBZ}.
FT   STRAND       47     51       {ECO:0000244|PDB:2DBQ}.
FT   HELIX        59     63       {ECO:0000244|PDB:2DBQ}.
FT   STRAND       70     76       {ECO:0000244|PDB:2DBQ}.
FT   HELIX        83     88       {ECO:0000244|PDB:2DBQ}.
FT   STRAND       92     94       {ECO:0000244|PDB:2DBQ}.
FT   STRAND       98    100       {ECO:0000244|PDB:2DBZ}.
FT   HELIX       101    117       {ECO:0000244|PDB:2DBQ}.
FT   HELIX       119    127       {ECO:0000244|PDB:2DBQ}.
FT   HELIX       130    133       {ECO:0000244|PDB:2DBQ}.
FT   TURN        140    143       {ECO:0000244|PDB:2DBQ}.
FT   STRAND      152    156       {ECO:0000244|PDB:2DBQ}.
FT   HELIX       160    171       {ECO:0000244|PDB:2DBQ}.
FT   STRAND      175    179       {ECO:0000244|PDB:2DBQ}.
FT   STRAND      181    184       {ECO:0000244|PDB:2DBR}.
FT   HELIX       185    191       {ECO:0000244|PDB:2DBQ}.
FT   STRAND      193    195       {ECO:0000244|PDB:2DBZ}.
FT   HELIX       198    204       {ECO:0000244|PDB:2DBQ}.
FT   STRAND      206    210       {ECO:0000244|PDB:2DBQ}.
FT   TURN        216    220       {ECO:0000244|PDB:2DBQ}.
FT   HELIX       224    229       {ECO:0000244|PDB:2DBQ}.
FT   STRAND      235    238       {ECO:0000244|PDB:2DBQ}.
FT   HELIX       242    244       {ECO:0000244|PDB:2DBQ}.
FT   HELIX       247    255       {ECO:0000244|PDB:2DBQ}.
FT   STRAND      258    265       {ECO:0000244|PDB:2DBQ}.
FT   STRAND      268    271       {ECO:0000244|PDB:2DBQ}.
FT   HELIX       275    279       {ECO:0000244|PDB:2DBQ}.
FT   STRAND      283    285       {ECO:0000244|PDB:2DBQ}.
FT   HELIX       294    312       {ECO:0000244|PDB:2DBQ}.
FT   HELIX       325    328       {ECO:0000244|PDB:2DBQ}.
SQ   SEQUENCE   334 AA;  38000 MW;  C0056A354ECBE202 CRC64;
     MKPKVFITRE IPEVGIKMLE DEFEVEVWGD EKEIPREILL KKVKEVDALV TMLSERIDKE
     VFENAPKLRI VANYAVGYDN IDIEEATKRG IYVTNTPDVL TDATADLAFA LLLATARHVV
     KGDRFVRSGE WKKRGVAWHP KWFLGYDVYG KTIGIIGLGR IGQAIAKRAK GFNMRILYYS
     RTRKEEVERE LNAEFKPLED LLRESDFVVL AVPLTRETYH LINEERLKLM KKTAILINIA
     RGKVVDTNAL VKALKEGWIA GAGLDVFEEE PYYNEELFKL DNVVLTPHIG SASFGAREGM
     AELVAKNLIA FKRGEIPPTL VNREVIKIRK PGFE
//
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