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Database: UniProt
Entry: O59025
LinkDB: O59025
Original site: O59025 
ID   HELS_PYRHO              Reviewed;         715 AA.
AC   O59025;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   11-DEC-2019, entry version 119.
DE   RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00442};
GN   Name=hel308 {ECO:0000255|HAMAP-Rule:MF_00442}; OrderedLocusNames=PH1280;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS   100139 / OT-3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA   Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA   Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA   Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA   Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC       involved in repair of stalled replication forks. {ECO:0000255|HAMAP-
CC       Rule:MF_00442}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00442}.
CC   -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00442}.
DR   EMBL; BA000001; BAA30383.1; -; Genomic_DNA.
DR   PIR; E71073; E71073.
DR   RefSeq; WP_010885366.1; NC_000961.1.
DR   SMR; O59025; -.
DR   STRING; 70601.3257700; -.
DR   PRIDE; O59025; -.
DR   EnsemblBacteria; BAA30383; BAA30383; BAA30383.
DR   GeneID; 1443602; -.
DR   KEGG; pho:PH1280; -.
DR   eggNOG; arCOG00553; Archaea.
DR   eggNOG; COG1204; LUCA.
DR   HOGENOM; HOG000227028; -.
DR   KO; K03726; -.
DR   OMA; NSNERGD; -.
DR   OrthoDB; 9752at2157; -.
DR   BioCyc; PHOR70601:G1G39-1238-MONOMER; -.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00442; Helicase_Hel308; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR022965; Helicase_Hel308.
DR   InterPro; IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00278; HhH1; 2.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding.
FT   CHAIN           1..715
FT                   /note="ATP-dependent DNA helicase Hel308"
FT                   /id="PRO_0000102111"
FT   DOMAIN          33..197
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   DOMAIN          229..422
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   NP_BIND         46..53
FT                   /note="ATP"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   MOTIF           1..29
FT                   /note="Q motif"
FT   MOTIF           145..148
FT                   /note="DEAH box"
FT   BINDING         28
FT                   /note="ATP"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
SQ   SEQUENCE   715 AA;  82106 MW;  F0E2A1AC765C999C CRC64;
     MKVEELRIDE RIKEVLKKRG ISELYPPQAE ALTSGILKGE NALIAIPTAS GKTLIAEIAI
     VNRLLKEGGK AVYLVPLKAL AEEKFKEFKD WEELGLKVAM ATGDYDSKDE WLGGYDIIIA
     TAEKFDSLLR HGSSWIRNVK VLVVDEIHLI GSRDRGATLE FIITQMLNRA QIIGLSATIG
     NPEELAEWLN AKLIKSDWRP VKLRRGVFYQ GFVFWEDGKT EKFNSWEELV YDAIKRSKGS
     LVFVNMRRKA EKTALELSKK IRNFLTKKEL RELKELAESL EENPTNEKLA KALQGGVAFH
     HAGLGREERV LVEENFKKGL IKVVVATPTL SAGINTPAFR VIVRDTWRYS EFGMERIPIL
     EIQQMMGRAG RPKYDEVGEA IIVSTTEEPS TVMERYIKGK PEKLFSQLSN ESILRGQILA
     LIATFNFSSF REIYDFLERT FYAYQGKDPY TLEDRIRDIV YFLLENEFIE ITLDDEIKAL
     PLGIRTAKLY IDPMTAKIFK DTLPKIEKDP NPLGILHVIS LTPDLIPLPY GKKELPMLED
     EYYSFKDRLY FELDWEDERK FLRAFKTALV LNAWINEVPE GEIVEKFNVE PGDIYRIVET
     AEWLVYSLKE IAKTLEYSQD VINYLETLRV RVKHGIREEL IPLMELPMIG RKRARALYNA
     GFRDLESIKN ARPAELLEVE GIGAKIVEAI LKHLGREVKI VQKPRKGTLD YYLHP
//
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