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Database: UniProt
Entry: O59828
LinkDB: O59828
Original site: O59828 
ID   ALR1_SCHPO              Reviewed;         375 AA.
AC   O59828;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   16-JAN-2019, entry version 120.
DE   RecName: Full=Alanine racemase, catabolic;
DE            EC=5.1.1.1;
GN   Name=alr1; ORFNames=SPCC965.08c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales;
OC   Schizosaccharomycetaceae; Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA   Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA   Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA   Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA   James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA   Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA   Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA   Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA   Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA   Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA   Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA   Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA   Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA   Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA   Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA   Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA   Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA   Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA   Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA   Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA   Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   CHARACTERIZATION.
RC   STRAIN=SP1;
RX   PubMed=11244061; DOI=10.1128/JB.183.7.2226-2233.2001;
RA   Uo T., Yoshimura T., Tanaka N., Takegawa K., Esaki N.;
RT   "Functional characterization of alanine racemase from
RT   Schizosaccharomyces pombe: a eucaryotic counterpart to bacterial
RT   alanine racemase.";
RL   J. Bacteriol. 183:2226-2233(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-alanine = D-alanine; Xref=Rhea:RHEA:20249,
CC         ChEBI:CHEBI:57416, ChEBI:CHEBI:57972; EC=5.1.1.1;
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 9.0-9.5.;
CC   -!- PATHWAY: Amino-acid biosynthesis; D-alanine biosynthesis; D-
CC       alanine from L-alanine: step 1/1.
CC   -!- SIMILARITY: Belongs to the alanine racemase family. {ECO:0000305}.
DR   EMBL; CU329672; CAA19068.1; -; Genomic_DNA.
DR   PIR; T41661; T41661.
DR   RefSeq; NP_588518.1; NM_001023507.2.
DR   ProteinModelPortal; O59828; -.
DR   SMR; O59828; -.
DR   BioGrid; 275325; 1.
DR   STRING; 4896.SPCC965.08c.1; -.
DR   PaxDb; O59828; -.
DR   PRIDE; O59828; -.
DR   EnsemblFungi; SPCC965.08c.1; SPCC965.08c.1:pep; SPCC965.08c.
DR   GeneID; 2538742; -.
DR   KEGG; spo:SPCC965.08c; -.
DR   EuPathDB; FungiDB:SPCC965.08c; -.
DR   PomBase; SPCC965.08c; alr1.
DR   HOGENOM; HOG000031446; -.
DR   InParanoid; O59828; -.
DR   KO; K01775; -.
DR   PhylomeDB; O59828; -.
DR   BRENDA; 5.1.1.1; 5613.
DR   UniPathway; UPA00042; UER00497.
DR   PRO; PR:O59828; -.
DR   Proteomes; UP000002485; Chromosome III.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0008784; F:alanine racemase activity; IDA:PomBase.
DR   GO; GO:0030378; F:serine racemase activity; IDA:PomBase.
DR   GO; GO:0006522; P:alanine metabolic process; IDA:PomBase.
DR   GO; GO:0030632; P:D-alanine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.40.37.10; -; 1.
DR   Gene3D; 3.20.20.10; -; 1.
DR   HAMAP; MF_01201; Ala_racemase; 1.
DR   InterPro; IPR000821; Ala_racemase.
DR   InterPro; IPR009006; Ala_racemase/Decarboxylase_C.
DR   InterPro; IPR011079; Ala_racemase_C.
DR   InterPro; IPR001608; Ala_racemase_N.
DR   InterPro; IPR020622; Ala_racemase_pyridoxalP-BS.
DR   InterPro; IPR029066; PLP-binding_barrel.
DR   Pfam; PF00842; Ala_racemase_C; 1.
DR   Pfam; PF01168; Ala_racemase_N; 1.
DR   PRINTS; PR00992; ALARACEMASE.
DR   SMART; SM01005; Ala_racemase_C; 1.
DR   SUPFAM; SSF50621; SSF50621; 1.
DR   SUPFAM; SSF51419; SSF51419; 1.
DR   TIGRFAMs; TIGR00492; alr; 1.
DR   PROSITE; PS00395; ALANINE_RACEMASE; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Isomerase; Pyridoxal phosphate; Reference proteome.
FT   CHAIN         1    375       Alanine racemase, catabolic.
FT                                /FTId=PRO_0000114604.
FT   ACT_SITE     38     38       Proton acceptor; specific for D-alanine.
FT                                {ECO:0000250}.
FT   ACT_SITE    269    269       Proton acceptor; specific for L-alanine.
FT                                {ECO:0000250}.
FT   MOD_RES      38     38       N6-(pyridoxal phosphate)lysine.
FT                                {ECO:0000250}.
SQ   SEQUENCE   375 AA;  41585 MW;  76CEBD17E159E13F CRC64;
     MRGAKSVIDL HAIAHNYNVA KQMMLQKNPS GHVLAIVKAN AYGHGAVQVA RFLLKHCSSI
     DGFGVSSIEE ALELRHGGIY NKIVLLEGFF TEEDELKLID DYNFSIIIHS EDQVNSFIKY
     PFNRPVEIWL KLDSGMNRLG FTPSQFMKFY NLLSNNKNVS NIGKITHFAF ADMLENPEHT
     LKQWDIFEKS VAHLPGPLSA GGSAIILGWL NTVCTDWLRA GIMLYGISPF LSKNKDSKTP
     ESVNIKPAMK LVSTIISVKH VDKGQPIGYG GRYVATRDMK LGVVAMGYGD GFPRQVKDGC
     PVLVNGVKAP IVGRVSMDML TVDLSDIPDV KPGDEVIFWG TPELTVADIA KYCSDTSPYE
     LVTKLTRRVP LQYTY
//
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