ID LRX1_ARATH Reviewed; 744 AA.
AC O65375;
DT 13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 27-MAR-2024, entry version 149.
DE RecName: Full=Leucine-rich repeat extensin-like protein 1;
DE Short=AtLRX1;
DE Short=LRR/EXTENSIN1;
DE AltName: Full=Cell wall hydroxyproline-rich glycoprotein;
DE Flags: Precursor;
GN Name=LRX1; OrderedLocusNames=At1g12040; ORFNames=F12F1.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=11331608; DOI=10.1101/gad.200201;
RA Baumberger N., Ringli C., Keller B.;
RT "The chimeric leucine-rich repeat/extensin cell wall protein LRX1 is
RT required for root hair morphogenesis in Arabidopsis thaliana.";
RL Genes Dev. 15:1128-1139(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=12834403; DOI=10.1046/j.1365-313x.2003.01784.x;
RA Baumberger N., Steiner M., Ryser U., Keller B., Ringli C.;
RT "Synergistic interaction of the two paralogous Arabidopsis genes LRX1 and
RT LRX2 in cell wall formation during root hair development.";
RL Plant J. 35:71-81(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12644681; DOI=10.1104/pp.102.014928;
RA Baumberger N., Doesseger B., Guyot R., Diet A., Parsons R.L., Clark M.A.,
RA Simmons M.P., Bedinger P., Goff S.A., Ringli C., Keller B.;
RT "Whole-genome comparison of leucine-rich repeat extensins in Arabidopsis
RT and rice. A conserved family of cell wall proteins form a vegetative and a
RT reproductive clade.";
RL Plant Physiol. 131:1313-1326(2003).
CC -!- FUNCTION: Modulates cell morphogenesis by regulating cell wall
CC formation and assembly, and/or growth polarization. Together with LRX2,
CC component of the extracellular mechanism regulating root hair
CC morphogenesis and elongation. {ECO:0000269|PubMed:11331608,
CC ECO:0000269|PubMed:12834403}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000269|PubMed:11331608}. Note=Specifically localized in the wall
CC of the root hair proper.
CC -!- TISSUE SPECIFICITY: Expressed in root hair cells (at protein level).
CC {ECO:0000269|PubMed:11331608}.
CC -!- DEVELOPMENTAL STAGE: First observed in differentiating trichoblast and
CC later confined to root hair proper. Soluble in the early stages of root
CC hair development and becomes insolubilized in later stages.
CC {ECO:0000269|PubMed:11331608}.
CC -!- PTM: Hydroxylated on proline residues in the S-P-P-P-P repeat.
CC {ECO:0000250}.
CC -!- PTM: O-glycosylated on hydroxyprolines. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Irregular root hair development that frequently
CC abort, swell, or branch. Stronger phenotype when associated with LRX2
CC disruption; frequent rupture of root hairs soon after their initiation.
CC {ECO:0000269|PubMed:11331608, ECO:0000269|PubMed:12834403}.
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DR EMBL; AY026364; AAK07681.1; -; mRNA.
DR EMBL; AC002131; AAC17609.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28829.1; -; Genomic_DNA.
DR PIR; E86255; E86255.
DR RefSeq; NP_172668.1; NM_101076.2.
DR AlphaFoldDB; O65375; -.
DR SMR; O65375; -.
DR STRING; 3702.O65375; -.
DR GlyCosmos; O65375; 5 sites, No reported glycans.
DR PaxDb; 3702-AT1G12040-1; -.
DR ProteomicsDB; 238635; -.
DR EnsemblPlants; AT1G12040.1; AT1G12040.1; AT1G12040.
DR GeneID; 837756; -.
DR Gramene; AT1G12040.1; AT1G12040.1; AT1G12040.
DR KEGG; ath:AT1G12040; -.
DR Araport; AT1G12040; -.
DR TAIR; AT1G12040; LRX1.
DR eggNOG; ENOG502QQD2; Eukaryota.
DR HOGENOM; CLU_000288_23_3_1; -.
DR InParanoid; O65375; -.
DR OMA; TYADNFI; -.
DR OrthoDB; 691476at2759; -.
DR PRO; PR:O65375; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O65375; baseline and differential.
DR Genevisible; O65375; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009505; C:plant-type cell wall; IDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0005199; F:structural constituent of cell wall; ISS:TAIR.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0010054; P:trichoblast differentiation; IMP:TAIR.
DR GO; GO:0009826; P:unidimensional cell growth; IMP:TAIR.
DR Gene3D; 3.80.10.10; Ribonuclease Inhibitor; 2.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR013210; LRR_N_plant-typ.
DR PANTHER; PTHR32093:SF116; LEUCINE-RICH REPEAT EXTENSIN-LIKE PROTEIN 1; 1.
DR PANTHER; PTHR32093; LEUCINE-RICH REPEAT EXTENSIN-LIKE PROTEIN 3-RELATED; 1.
DR Pfam; PF00560; LRR_1; 3.
DR Pfam; PF08263; LRRNT_2; 1.
DR PRINTS; PR01217; PRICHEXTENSN.
DR SUPFAM; SSF52058; L domain-like; 1.
PE 1: Evidence at protein level;
KW Cell wall; Cell wall biogenesis/degradation; Developmental protein;
KW Glycoprotein; Hydroxylation; Leucine-rich repeat; Reference proteome;
KW Repeat; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..744
FT /note="Leucine-rich repeat extensin-like protein 1"
FT /id="PRO_0000395461"
FT REPEAT 122..145
FT /note="LRR 1"
FT REPEAT 147..170
FT /note="LRR 2"
FT REPEAT 171..194
FT /note="LRR 3"
FT REPEAT 196..217
FT /note="LRR 4"
FT REPEAT 219..240
FT /note="LRR 5"
FT REPEAT 241..265
FT /note="LRR 6"
FT REPEAT 266..289
FT /note="LRR 7"
FT REPEAT 290..313
FT /note="LRR 8"
FT REPEAT 315..336
FT /note="LRR 9"
FT REPEAT 381..404
FT /note="LRR 10"
FT REGION 382..744
FT /note="Contains the Ser-Pro(4) repeats"
FT REGION 408..445
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 518..537
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 555..576
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 658..744
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 658..682
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 700..744
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 71
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 77
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 253
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 318
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 344
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 744 AA; 80994 MW; 3C75174DBBB91F6F CRC64;
MLFPPLRSLF LFTLLLSSVC FLQIKADHDD ESDLGSDIKV DKRLKFENPK LRQAYIALQS
WKKAIFSDPF NFTANWNGSD VCSYNGIYCA PSPSYPKTRV VAGIDLNHAD MAGYLASELG
LLSDLALFHI NSNRFCGEVP LTFNRMKLLY ELDLSNNRFV GKFPKVVLSL PSLKFLDLRY
NEFEGKIPSK LFDRELDAIF LNHNRFRFGI PKNMGNSPVS ALVLADNNLG GCIPGSIGQM
GKTLNELILS NDNLTGCLPP QIGNLKKVTV FDITSNRLQG PLPSSVGNMK SLEELHVANN
AFTGVIPPSI CQLSNLENFT YSSNYFSGRP PICAASLLAD IVVNGTMNCI TGLARQRSDK
QCSSLLARPV DCSKFGCYNI FSPPPPTFKM SPEVRTLPPP IYVYSSPPPP PSSKMSPTVR
AYSPPPPPSS KMSPSVRAYS PPPPPYSKMS PSVRAYPPPP PPSPSPPPPY VYSSPPPPYV
YSSPPPPPYV YSSPPPPPYV YSSPPPPYVY SSPPPPYVYS SPPPPPPSPP PPCPESSPPP
PVVYYAPVTQ SPPPPSPVYY PPVTQSPPPP SPVYYPPVTN SPPPPSPVYY PPVTYSPPPP
SPVYYPQVTP SPPPPSPLYY PPVTPSPPPP SPVYYPPVTP SPPPPSPVYY PPVTPSPPPP
SPVYYPSETQ SPPPPTEYYY SPSQSPPPTK ACKEGHPPQA TPSYEPPPEY SYSSSPPPPS
PTSYFPPMPS VSYDASPPPP PSYY
//
