GenomeNet

Database: UniProt
Entry: O70273
LinkDB: O70273
Original site: O70273 
ID   EHF_MOUSE               Reviewed;         300 AA.
AC   O70273; Q922E8; Q922T6; Q99K12;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   12-AUG-2020, entry version 146.
DE   RecName: Full=ETS homologous factor;
DE   AltName: Full=ETS domain-containing transcription factor;
GN   Name=Ehf {ECO:0000312|MGI:MGI:1270840};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAC40119.1}
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC   TISSUE=Pituitary {ECO:0000312|EMBL:AAC40119.1};
RX   PubMed=9600089; DOI=10.1006/bbrc.1998.8560;
RA   Bochert M.A., Kleinbaum L.A., Sun L.-Y., Burton F.H.;
RT   "Molecular cloning and expression of Ehf, a new member of the ets
RT   transcription factor/oncoprotein gene family.";
RL   Biochem. Biophys. Res. Commun. 246:176-181(1998).
RN   [2] {ECO:0000305, ECO:0000312|EMBL:AAH05520.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), AND NUCLEOTIDE
RP   SEQUENCE [LARGE SCALE MRNA] OF 222-300 (ISOFORMS 1/2).
RC   STRAIN=Czech II {ECO:0000312|EMBL:AAH05520.1}, and
RC   FVB/N {ECO:0000312|EMBL:AAH08249.1};
RC   TISSUE=Mammary gland {ECO:0000312|EMBL:AAH05520.1};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3] {ECO:0000305}
RP   DOMAIN.
RX   PubMed=16704374; DOI=10.1042/bj20060375;
RA   Choi Y.S., Sinha S.;
RT   "Determination of the consensus DNA-binding sequence and a transcriptional
RT   activation domain for ESE-2.";
RL   Biochem. J. 398:497-507(2006).
CC   -!- FUNCTION: Transcriptional activator that may play a role in regulating
CC       epithelial cell differentiation and proliferation. May act as a
CC       repressor for a specific subset of ETS/AP-1-responsive genes, and as a
CC       modulator of the nuclear response to mitogen-activated protein kinase
CC       signaling cascades. Binds to DNA sequences containing the consensus
CC       nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5
CC       expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter
CC       (By similarity). {ECO:0000250|UniProtKB:Q9NZC4,
CC       ECO:0000269|PubMed:16704374}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9NZC4,
CC       ECO:0000255|PROSITE-ProRule:PRU00237}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1 {ECO:0000269|PubMed:9600089};
CC         IsoId=O70273-1; Sequence=Displayed;
CC       Name=2 {ECO:0000269|PubMed:15489334};
CC         IsoId=O70273-2; Sequence=VSP_052191;
CC       Name=3 {ECO:0000269|PubMed:15489334};
CC         IsoId=O70273-3; Sequence=VSP_052192, VSP_052193;
CC   -!- TISSUE SPECIFICITY: Highly expressed in kidney and lung, weakly in
CC       skeletal muscle, heart, and liver, and not detected in brain, spleen or
CC       testis. {ECO:0000269|PubMed:9600089}.
CC   -!- DOMAIN: The PNT domain acts as a transcriptional activator.
CC       {ECO:0000269|PubMed:16704374}.
CC   -!- SIMILARITY: Belongs to the ETS family. {ECO:0000255}.
DR   EMBL; AF035527; AAC40119.1; -; mRNA.
DR   EMBL; BC005520; AAH05520.1; -; mRNA.
DR   EMBL; BC006789; AAH06789.1; -; mRNA.
DR   EMBL; BC008249; AAH08249.1; -; mRNA.
DR   CCDS; CCDS16475.1; -. [O70273-1]
DR   PIR; JW0048; JW0048.
DR   RefSeq; NP_031940.1; NM_007914.3. [O70273-1]
DR   RefSeq; XP_006498760.1; XM_006498697.2. [O70273-1]
DR   RefSeq; XP_006498761.1; XM_006498698.3. [O70273-1]
DR   RefSeq; XP_006498762.1; XM_006498699.1. [O70273-1]
DR   RefSeq; XP_006498763.1; XM_006498700.3. [O70273-1]
DR   RefSeq; XP_006498764.1; XM_006498701.3. [O70273-1]
DR   RefSeq; XP_006498765.1; XM_006498702.3. [O70273-1]
DR   BioGRID; 199409; 1.
DR   STRING; 10090.ENSMUSP00000087961; -.
DR   PhosphoSitePlus; O70273; -.
DR   MaxQB; O70273; -.
DR   PaxDb; O70273; -.
DR   PeptideAtlas; O70273; -.
DR   PRIDE; O70273; -.
DR   Antibodypedia; 13027; 128 antibodies.
DR   Ensembl; ENSMUST00000090475; ENSMUSP00000087961; ENSMUSG00000012350. [O70273-1]
DR   Ensembl; ENSMUST00000111174; ENSMUSP00000106805; ENSMUSG00000012350. [O70273-1]
DR   GeneID; 13661; -.
DR   KEGG; mmu:13661; -.
DR   UCSC; uc008lio.1; mouse. [O70273-1]
DR   CTD; 26298; -.
DR   MGI; MGI:1270840; Ehf.
DR   eggNOG; KOG3804; Eukaryota.
DR   GeneTree; ENSGT00940000159310; -.
DR   InParanoid; O70273; -.
DR   KO; K17102; -.
DR   OrthoDB; 837296at2759; -.
DR   PhylomeDB; O70273; -.
DR   TreeFam; TF318679; -.
DR   BioGRID-ORCS; 13661; 1 hit in 18 CRISPR screens.
DR   ChiTaRS; Ehf; mouse.
DR   PRO; PR:O70273; -.
DR   Proteomes; UP000000589; Chromosome 2.
DR   RNAct; O70273; protein.
DR   Bgee; ENSMUSG00000012350; Expressed in secondary oocyte and 174 other tissues.
DR   ExpressionAtlas; O70273; baseline and differential.
DR   Genevisible; O70273; MM.
DR   GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR   GO; GO:0042025; C:host cell nucleus; IEA:InterPro.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR   GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR   GO; GO:0030855; P:epithelial cell differentiation; ISS:UniProtKB.
DR   GO; GO:0050673; P:epithelial cell proliferation; IEA:InterPro.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 1.10.150.50; -; 1.
DR   InterPro; IPR033071; EHF.
DR   InterPro; IPR000418; Ets_dom.
DR   InterPro; IPR003118; Pointed_dom.
DR   InterPro; IPR013761; SAM/pointed_sf.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR11849:SF171; PTHR11849:SF171; 1.
DR   Pfam; PF00178; Ets; 1.
DR   Pfam; PF02198; SAM_PNT; 1.
DR   PRINTS; PR00454; ETSDOMAIN.
DR   SMART; SM00413; ETS; 1.
DR   SMART; SM00251; SAM_PNT; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF47769; SSF47769; 1.
DR   PROSITE; PS50061; ETS_DOMAIN_3; 1.
DR   PROSITE; PS51433; PNT; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..300
FT                   /note="ETS homologous factor"
FT                   /id="PRO_0000257970"
FT   DOMAIN          29..115
FT                   /note="PNT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00762"
FT   DNA_BIND        207..289
FT                   /note="ETS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00237"
FT   VAR_SEQ         79..93
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_052191"
FT   VAR_SEQ         200..211
FT                   /note="KKHNPRGTHLWE -> YFIIELLLSESR (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_052192"
FT   VAR_SEQ         212..300
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_052193"
SQ   SEQUENCE   300 AA;  34903 MW;  91D9477EFB874747 CRC64;
     MILEGSGVMN LNPANNLLHQ QPAWTDSYPT CNVSSGFFGS QWHEIHPQYW TKYQVWEWLQ
     HLLDTNQLDA SCIPFQEFDI SGEHLCSMSL QEFTRAAGSA GQLLYSNLQH LKWNGQCSSD
     LFQSAHNVIV KTEQTDPSIM NTWKEENYLY DPSYGSTVDL LDSKTFCRAQ ISMTTSSHLP
     VAESPDMKKE QDHPVKSHTK KHNPRGTHLW EFIRDILLSP DKNPGLIKWE DRSEGIFRFL
     KSEAVAQLWG KKKNNSSMTY EKLSRAMRYY YKREILERVD GRRLVYKFGK NARGWRENEN
//
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