ID OR2J3_HUMAN Reviewed; 311 AA.
AC O76001; B0UY52; B9EH11; Q5SUJ7; Q6IF25; Q96R15; Q9GZK5; Q9GZL4; Q9GZL5;
DT 20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 27-MAR-2024, entry version 190.
DE RecName: Full=Olfactory receptor 2J3;
DE AltName: Full=Hs6M1-3;
DE AltName: Full=Olfactory receptor OR6-16;
DE Short=OR6-6;
DE Short=Olfactory receptor 6-6;
GN Name=OR2J3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS ALA-113; GLN-226; ILE-228
RP AND MET-261.
RA Ziegler A., Ehlers A., Forbes S.A., Trowsdale J., Uchanska-Ziegler B.,
RA Volz A., Younger R., Beck S.;
RT "Polymorphic olfactory receptor genes and HLA loci constitute extended
RT haplotypes.";
RL (In) Kasahara M. (eds.);
RL Major histocompatibility complex-evolution, structure, and function,
RL pp.110-130, Springer-Verlag, Tokyo (2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT MET-261.
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT MET-261.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 71-286.
RX PubMed=12213199; DOI=10.1006/geno.2002.6830;
RA Fuchs T., Malecova B., Linhart C., Sharan R., Khen M., Herwig R.,
RA Shmulevich D., Elkon R., Steinfath M., O'Brien J.K., Radelof U.,
RA Lehrach H., Lancet D., Shamir R.;
RT "DEFOG: a practical scheme for deciphering families of genes.";
RL Genomics 80:295-302(2002).
RN [5]
RP IDENTIFICATION.
RX PubMed=14983052; DOI=10.1073/pnas.0307882100;
RA Malnic B., Godfrey P.A., Buck L.B.;
RT "The human olfactory receptor gene family.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:2584-2589(2004).
RN [6]
RP ERRATUM OF PUBMED:14983052.
RA Malnic B., Godfrey P.A., Buck L.B.;
RL Proc. Natl. Acad. Sci. U.S.A. 101:7205-7205(2004).
RN [7]
RP FUNCTION, POLYMORPHISM, VARIANTS ALA-113 AND GLN-226, AND CHARACTERIZATION
RP OF VARIANTS ALA-113 AND GLN-226.
RX PubMed=22714804; DOI=10.1093/chemse/bjs049;
RA McRae J.F., Mainland J.D., Jaeger S.R., Adipietro K.A., Matsunami H.,
RA Newcomb R.D.;
RT "Genetic variation in the odorant receptor OR2J3 is associated with the
RT ability to detect the 'grassy' smelling odor, cis-3-hexen-1-ol.";
RL Chem. Senses 37:585-593(2012).
CC -!- FUNCTION: Odorant receptor involved in the detection of the flavor
CC compound cis-3-hexen-1-ol (C3HEX), a compound typically described as
CC 'green grassy' or the smell of 'cut grass'.
CC {ECO:0000269|PubMed:22714804}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- POLYMORPHISM: Variants Ala-113 and Gln-226 exhibit a reduced ability to
CC smell C3HEX [MIM:615082]. When both variants are present in the same
CC haplotype (allele 6M1-3*02), the response to C3HEX is abolished.
CC African populations have a higher proportion of the C3HEX-nonresponsive
CC haplotype than other populations. All haplotypes containing Gln-226
CC show low levels of surface expression. {ECO:0000269|PubMed:22714804}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- WEB RESOURCE: Name=Human Olfactory Receptor Data Exploratorium (HORDE);
CC URL="http://genome.weizmann.ac.il/horde/card/index/symbol:OR2J3";
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=The intricacy of a smell
CC - Issue 150 of July 2013;
CC URL="https://web.expasy.org/spotlight/back_issues/150";
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DR EMBL; AJ302547; CAC20472.1; -; Genomic_DNA.
DR EMBL; AJ302548; CAC20473.1; -; Genomic_DNA.
DR EMBL; AJ302549; CAC20474.1; -; Genomic_DNA.
DR EMBL; AJ302550; CAC20475.1; -; Genomic_DNA.
DR EMBL; AJ302551; CAC20476.1; -; Genomic_DNA.
DR EMBL; AJ302552; CAC21440.1; -; Genomic_DNA.
DR EMBL; AJ302553; CAC21441.1; -; Genomic_DNA.
DR EMBL; AJ302554; CAC21442.1; -; Genomic_DNA.
DR EMBL; AJ302555; CAC21443.1; -; Genomic_DNA.
DR EMBL; AJ302556; CAC21444.1; -; Genomic_DNA.
DR EMBL; AJ302557; CAC20477.1; -; Genomic_DNA.
DR EMBL; AJ302558; CAC20478.1; -; Genomic_DNA.
DR EMBL; AL645937; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL022727; CAA18783.1; -; Genomic_DNA.
DR EMBL; AL662852; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL929561; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX248093; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CR547123; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CR388415; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CR942175; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC136973; AAI36974.1; -; mRNA.
DR EMBL; BC136975; AAI36976.1; -; mRNA.
DR EMBL; AF399630; AAK95115.1; -; Genomic_DNA.
DR EMBL; BK004437; DAA04835.1; -; Genomic_DNA.
DR CCDS; CCDS43433.1; -.
DR RefSeq; NP_001005216.2; NM_001005216.3.
DR AlphaFoldDB; O76001; -.
DR SMR; O76001; -.
DR STRING; 9606.ENSP00000493439; -.
DR GlyCosmos; O76001; 1 site, No reported glycans.
DR GlyGen; O76001; 1 site.
DR BioMuta; OR2J3; -.
DR PaxDb; 9606-ENSP00000366374; -.
DR PeptideAtlas; O76001; -.
DR ProteomicsDB; 50339; -.
DR Antibodypedia; 62681; 89 antibodies from 17 providers.
DR DNASU; 442186; -.
DR Ensembl; ENST00000377169.2; ENSP00000366374.1; ENSG00000204701.3.
DR Ensembl; ENST00000383651.1; ENSP00000373147.1; ENSG00000206522.1.
DR Ensembl; ENST00000414140.1; ENSP00000390144.1; ENSG00000232178.1.
DR Ensembl; ENST00000418630.1; ENSP00000400546.1; ENSG00000233618.1.
DR Ensembl; ENST00000419260.1; ENSP00000408783.1; ENSG00000229866.1.
DR Ensembl; ENST00000428081.1; ENSP00000388392.1; ENSG00000226271.1.
DR Ensembl; ENST00000430478.1; ENSP00000388205.1; ENSG00000233636.1.
DR GeneID; 442186; -.
DR KEGG; hsa:442186; -.
DR UCSC; uc011dll.4; human.
DR AGR; HGNC:8261; -.
DR CTD; 442186; -.
DR DisGeNET; 442186; -.
DR GeneCards; OR2J3; -.
DR HGNC; HGNC:8261; OR2J3.
DR HPA; ENSG00000204701; Not detected.
DR MalaCards; OR2J3; -.
DR MIM; 615016; gene.
DR MIM; 615082; phenotype.
DR neXtProt; NX_O76001; -.
DR PharmGKB; PA32178; -.
DR VEuPathDB; HostDB:ENSG00000204701; -.
DR eggNOG; ENOG502RU1S; Eukaryota.
DR HOGENOM; CLU_012526_1_2_1; -.
DR InParanoid; O76001; -.
DR OrthoDB; 4790557at2759; -.
DR PhylomeDB; O76001; -.
DR TreeFam; TF336512; -.
DR PathwayCommons; O76001; -.
DR Reactome; R-HSA-381753; Olfactory Signaling Pathway.
DR Reactome; R-HSA-9752946; Expression and translocation of olfactory receptors.
DR BioGRID-ORCS; 442186; 13 hits in 710 CRISPR screens.
DR GeneWiki; OR2J3; -.
DR GenomeRNAi; 442186; -.
DR Pharos; O76001; Tbio.
DR PRO; PR:O76001; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; O76001; Protein.
DR Bgee; ENSG00000204701; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 3 other cell types or tissues.
DR ExpressionAtlas; O76001; baseline and differential.
DR Genevisible; O76001; HS.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0004984; F:olfactory receptor activity; IBA:GO_Central.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; IBA:GO_Central.
DR CDD; cd15947; 7tmA_OR2B-like; 1.
DR Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR000725; Olfact_rcpt.
DR PANTHER; PTHR26453; OLFACTORY RECEPTOR; 1.
DR PANTHER; PTHR26453:SF184; OLFACTORY RECEPTOR 2J3; 1.
DR Pfam; PF13853; 7tm_4; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00245; OLFACTORYR.
DR SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Olfaction; Receptor; Reference proteome; Sensory transduction;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..311
FT /note="Olfactory receptor 2J3"
FT /id="PRO_0000150484"
FT TOPO_DOM 1..28
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 29..52
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..60
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..82
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..103
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..123
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 124..142
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..161
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 162..198
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..222
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 223..239
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..262
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 263..275
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..295
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 296..311
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 8
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 100..192
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VARIANT 113
FT /note="T -> A (in allele 6M1-3*02; decreased response to
FT C3HEX; dbSNP:rs28757581)"
FT /evidence="ECO:0000269|PubMed:22714804, ECO:0000269|Ref.1"
FT /id="VAR_010949"
FT VARIANT 226
FT /note="R -> Q (in allele 6M1-3*02; decreased response to
FT C3HEX; dbSNP:rs3749977)"
FT /evidence="ECO:0000269|PubMed:22714804, ECO:0000269|Ref.1"
FT /id="VAR_010950"
FT VARIANT 228
FT /note="V -> I (in allele 6M1-3*03; dbSNP:rs3130764)"
FT /evidence="ECO:0000269|Ref.1"
FT /id="VAR_010951"
FT VARIANT 261
FT /note="I -> M (in allele 6M1-3*03 and allele 6M1-3*04;
FT dbSNP:rs3130765)"
FT /evidence="ECO:0000269|PubMed:14574404,
FT ECO:0000269|PubMed:15489334, ECO:0000269|Ref.1"
FT /id="VAR_010952"
SQ SEQUENCE 311 AA; 34950 MW; 84201145B9D5AA68 CRC64;
MNDDGKVNAS SEGYFILVGF SNWPHLEVVI FVVVLIFYLM TLIGNLFIII LSYLDSHLHT
PMYFFLSNLS FLDLCYTTSS IPQLLVNLWG PEKTISYAGC MIQLYFVLAL GTTECVLLVV
MSYDRYAAVC RPLHYTVLMH PRFCHLLAVA SWVSGFTNSA LHSSFTFWVP LCGHRQVDHF
FCEVPALLRL SCVDTHVNEL TLMITSSIFV LIPLILILTS YGAIVRAVLR MQSTTGLQKV
FGTCGAHLMA VSLFFIPAMC IYLQPPSGNS QDQGKFIALF YTVVTPSLNP LIYTLRNKVV
RGAVKRLMGW E
//
