ID CIA1_ARATH Reviewed; 352 AA.
AC O80990; F4ITL5;
DT 22-JUL-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 24-JAN-2024, entry version 158.
DE RecName: Full=Protein CIA1 {ECO:0000303|PubMed:23104832};
DE AltName: Full=Cytosolic iron-sulfur protein assembly 1 {ECO:0000303|PubMed:23104832};
DE AltName: Full=Protein EMBRYO DEFECTIVE 1345 {ECO:0000303|PubMed:15266054};
GN Name=CIA1 {ECO:0000303|PubMed:23104832};
GN Synonyms=EMB1345 {ECO:0000303|PubMed:15266054};
GN OrderedLocusNames=At2g26060 {ECO:0000312|Araport:AT2G26060};
GN ORFNames=T19L18.13 {ECO:0000312|EMBL:AAC31230.2};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP DISRUPTION PHENOTYPE.
RX PubMed=15266054; DOI=10.1104/pp.104.045179;
RA Tzafrir I., Pena-Muralla R., Dickerman A., Berg M., Rogers R., Hutchens S.,
RA Sweeney T.C., McElver J., Aux G., Patton D., Meinke D.;
RT "Identification of genes required for embryo development in Arabidopsis.";
RL Plant Physiol. 135:1206-1220(2004).
RN [6]
RP IDENTIFICATION, AND REVIEW.
RX PubMed=18493040; DOI=10.1534/genetics.107.086033;
RA Godman J., Balk J.;
RT "Genome analysis of Chlamydomonas reinhardtii reveals the existence of
RT multiple, compartmentalized iron-sulfur protein assembly machineries of
RT different evolutionary origins.";
RL Genetics 179:59-68(2008).
RN [7]
RP FUNCTION, INTERACTION WITH AE7 AND NAR1, SUBCELLULAR LOCATION, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=23104832; DOI=10.1105/tpc.112.102608;
RA Luo D., Bernard D.G., Balk J., Hai H., Cui X.;
RT "The DUF59 family gene AE7 acts in the cytosolic iron-sulfur cluster
RT assembly pathway to maintain nuclear genome integrity in Arabidopsis.";
RL Plant Cell 24:4135-4148(2012).
CC -!- FUNCTION: Essential component of the cytosolic iron-sulfur (Fe-S)
CC protein assembly (CIA) machinery. Required for the maturation of
CC extramitochondrial Fe/S proteins. {ECO:0000269|PubMed:23104832}.
CC -!- SUBUNIT: Part of a complex composed of AE7, CIA1, MMS19 and NAR1.
CC Interacts with AE7 and NAR1. {ECO:0000269|PubMed:23104832}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23104832}. Cytoplasm
CC {ECO:0000269|PubMed:23104832}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O80990-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O80990-2; Sequence=VSP_057803, VSP_057804;
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality when homozygous.
CC {ECO:0000269|PubMed:15266054, ECO:0000269|PubMed:23104832}.
CC -!- SIMILARITY: Belongs to the WD repeat CIA1 family. {ECO:0000305}.
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DR EMBL; AC004747; AAC31230.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC07790.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07791.1; -; Genomic_DNA.
DR EMBL; AY093154; AAM13153.1; -; mRNA.
DR EMBL; BT003394; AAO30057.1; -; mRNA.
DR EMBL; AY085277; AAM62509.1; -; mRNA.
DR PIR; T02617; T02617.
DR RefSeq; NP_001031421.1; NM_001036344.1. [O80990-2]
DR RefSeq; NP_565615.1; NM_128165.4. [O80990-1]
DR AlphaFoldDB; O80990; -.
DR SMR; O80990; -.
DR STRING; 3702.O80990; -.
DR PaxDb; 3702-AT2G26060-1; -.
DR ProteomicsDB; 246892; -. [O80990-1]
DR EnsemblPlants; AT2G26060.1; AT2G26060.1; AT2G26060. [O80990-1]
DR EnsemblPlants; AT2G26060.2; AT2G26060.2; AT2G26060. [O80990-2]
DR GeneID; 817147; -.
DR Gramene; AT2G26060.1; AT2G26060.1; AT2G26060. [O80990-1]
DR Gramene; AT2G26060.2; AT2G26060.2; AT2G26060. [O80990-2]
DR KEGG; ath:AT2G26060; -.
DR Araport; AT2G26060; -.
DR TAIR; AT2G26060; EMB1345.
DR eggNOG; KOG0645; Eukaryota.
DR HOGENOM; CLU_000288_57_8_1; -.
DR InParanoid; O80990; -.
DR OMA; IREIRWS; -.
DR OrthoDB; 5486866at2759; -.
DR PhylomeDB; O80990; -.
DR PRO; PR:O80990; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O80990; baseline and differential.
DR Genevisible; O80990; AT.
DR GO; GO:0097361; C:CIA complex; IEA:InterPro.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IGI:TAIR.
DR CDD; cd00200; WD40; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR HAMAP; MF_03037; ciao1; 1.
DR InterPro; IPR028608; CIAO1/Cia1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR19920:SF0; CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED; 1.
DR PANTHER; PTHR19920; WD40 PROTEIN CIAO1; 1.
DR Pfam; PF00400; WD40; 6.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 6.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Nucleus; Reference proteome; Repeat;
KW WD repeat.
FT CHAIN 1..352
FT /note="Protein CIA1"
FT /id="PRO_0000433517"
FT REPEAT 18..64
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 72..111
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 116..155
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 161..200
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 211..250
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 265..303
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 315..352
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT VAR_SEQ 329..337
FT /note="EGNRLLASA -> VREPVACVG (in isoform 2)"
FT /id="VSP_057803"
FT VAR_SEQ 338..352
FT /note="Missing (in isoform 2)"
FT /id="VSP_057804"
SQ SEQUENCE 352 AA; 39131 MW; DA691BB99CD67E12 CRC64;
MDLMEKNLEL VEIQKLEGHT DRVWSVAWNP VSSHADGVSP ILASCSGDNT VRIWEQSSLS
RSWTCKTVLE ETHTRTVRSC AWSPSGQLLA TASFDGTTGI WKNYGSEFEC ISTLEGHENE
VKSVSWNASG SCLATCSRDK SVWIWEVLEG NEYDCAAVLT GHTQDVKMVQ WHPTMDVLFS
CSYDNTIKVW WSEDDDGEYQ CVQTLGESNN GHSSTVWSIS FNAAGDKMVT CSDDLTLKIW
GTDIAKMQSG EEYAPWIHLC TLSGYHDRTI YSAHWSRDDI IASGAGDNAI RLFVDSKHDS
VDGPSYNLLL KKNKAHENDV NSVQWSPGEG NRLLASASDD GMVKIWQLAT KP
//
