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Database: UniProt
Entry: O81263
LinkDB: O81263
Original site: O81263 
ID   KITH_ORYSJ              Reviewed;         271 AA.
AC   O81263; Q8GZX9;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 2.
DT   13-FEB-2019, entry version 93.
DE   RecName: Full=Thymidine kinase;
DE            EC=2.7.1.21;
GN   Name=TK; OrderedLocusNames=Os03g0113100, LOC_Os03g02200;
GN   ORFNames=OSJNBa0090O10.3;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R.,
RA   Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L.,
RA   Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S., Johri S.,
RA   Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S.,
RA   Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J.,
RA   de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H.,
RA   Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F.,
RA   Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A.,
RA   Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J.,
RA   Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L.,
RA   Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A.,
RA   Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W.,
RA   Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome
RT   3 and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H.,
RA   McCombie W.R., Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S.,
RA   Childs K.L., Davidson R.M., Lin H., Quesada-Ocampo L.,
RA   Vaillancourt B., Sakai H., Lee S.S., Kim J., Numa H., Itoh T.,
RA   Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using
RT   next generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 3-271 (ISOFORM 2).
RC   STRAIN=cv. Nipponbare; TISSUE=Root;
RA   Ullah H., Robertson N., Fites R.C.;
RT   "A gene for thymidine kinase in plants.";
RL   (er) Plant Gene Register PGR99-048(1999).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + thymidine = ADP + dTMP + H(+);
CC         Xref=Rhea:RHEA:19129, ChEBI:CHEBI:15378, ChEBI:CHEBI:17748,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:63528, ChEBI:CHEBI:456216;
CC         EC=2.7.1.21;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=O81263-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=O81263-2; Sequence=VSP_018389;
CC         Note=May be due to a competing acceptor splice site. No
CC         experimental confirmation available.;
CC   -!- SIMILARITY: Belongs to the thymidine kinase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC31168.1; Type=Erroneous initiation; Evidence={ECO:0000305};
DR   EMBL; AC134237; AAO17013.1; -; Genomic_DNA.
DR   EMBL; AP014959; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AK066124; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AK068779; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AF066050; AAC31168.1; ALT_INIT; mRNA.
DR   PIR; T02888; T02888.
DR   UniGene; Os.2298; -.
DR   ProteinModelPortal; O81263; -.
DR   SMR; O81263; -.
DR   STRING; 39947.LOC_Os03g02200.1; -.
DR   PaxDb; O81263; -.
DR   eggNOG; KOG3125; Eukaryota.
DR   eggNOG; COG1435; LUCA.
DR   InParanoid; O81263; -.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; O81263; OS.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004797; F:thymidine kinase activity; IBA:GO_Central.
DR   GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0006259; P:DNA metabolic process; IBA:GO_Central.
DR   GO; GO:0046104; P:thymidine metabolic process; IBA:GO_Central.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001267; Thymidine_kinase.
DR   InterPro; IPR020633; Thymidine_kinase_CS.
DR   PANTHER; PTHR11441; PTHR11441; 1.
DR   Pfam; PF00265; TK; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00603; TK_CELLULAR_TYPE; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; ATP-binding; Complete proteome; DNA synthesis;
KW   Kinase; Metal-binding; Nucleotide-binding; Reference proteome;
KW   Transferase; Zinc.
FT   CHAIN         1    271       Thymidine kinase.
FT                                /FTId=PRO_0000174952.
FT   NP_BIND      74     81       ATP. {ECO:0000255}.
FT   NP_BIND     152    155       ATP. {ECO:0000250}.
FT   ACT_SITE    153    153       Proton acceptor. {ECO:0000255}.
FT   METAL       209    209       Zinc. {ECO:0000250}.
FT   METAL       212    212       Zinc. {ECO:0000250}.
FT   METAL       241    241       Zinc. {ECO:0000250}.
FT   BINDING     184    184       Substrate. {ECO:0000250}.
FT   BINDING     237    237       Substrate. {ECO:0000250}.
FT   VAR_SEQ      96     96       Missing (in isoform 2).
FT                                {ECO:0000303|PubMed:12869764,
FT                                ECO:0000303|Ref.5}.
FT                                /FTId=VSP_018389.
SQ   SEQUENCE   271 AA;  29456 MW;  1ABE3C95DB00E8B4 CRC64;
     MRSLLAASTF LRSGASPLLR PLSRPLPSRL NLSRFGPVRP VSAAAAAADK SRGGGGSAME
     AQPSYPGEIH VIVGPMFAGK TTALLRRVQV EAGTGSRNVA LIKSDKDNRY GLDSVVTHDG
     TKMPCWALPE LSSFQDKLGT EAYDKVDVIG IDEAQFFDDL HDFCCKAADR DGKIVVVAGL
     DGDYKRNKFG SVLDIIPLAD SVTKLTARCE LCGRRAFFTL RKTRETKTEL IGGADVYMPV
     CRQHYLDGQI VIEATRIVLD LEKSKVIHAF K
//
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