ID GLNA2_SOYBN Reviewed; 356 AA.
AC O82560;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 27-MAR-2024, entry version 118.
DE RecName: Full=Glutamine synthetase cytosolic isozyme 2;
DE EC=6.3.1.2;
DE AltName: Full=GS1-2;
DE AltName: Full=Glutamate--ammonia ligase;
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Resnik; TISSUE=Root nodule;
RA Morey K.J., Sengupta-Gopalan C.;
RT "Soybean gene coding for a nodule specific glutamine synthetase.";
RL (er) Plant Gene Register PGR98-194(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine +
CC phosphate; Xref=Rhea:RHEA:16169, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.1.2;
CC -!- SUBUNIT: Homooctamer. {ECO:0000250}.
CC -!- INTERACTION:
CC O82560; O82560: -; NbExp=4; IntAct=EBI-10192111, EBI-10192111;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- TISSUE SPECIFICITY: This is a nodule isozyme.
CC -!- SIMILARITY: Belongs to the glutamine synthetase family. {ECO:0000305}.
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DR EMBL; AF091456; AAC97935.1; -; Genomic_DNA.
DR RefSeq; XP_003544980.1; XM_003544932.3.
DR AlphaFoldDB; O82560; -.
DR SMR; O82560; -.
DR MINT; O82560; -.
DR STRING; 3847.O82560; -.
DR PaxDb; 3847-GLYMA14G39420-1; -.
DR ProMEX; O82560; -.
DR EnsemblPlants; KRH17323; KRH17323; GLYMA_14G213300.
DR Gramene; KRH17323; KRH17323; GLYMA_14G213300.
DR eggNOG; KOG0683; Eukaryota.
DR HOGENOM; CLU_036762_0_1_1; -.
DR InParanoid; O82560; -.
DR OrthoDB; 1115057at2759; -.
DR BRENDA; 6.3.1.2; 2483.
DR Proteomes; UP000008827; Chromosome 14.
DR Genevisible; O82560; GM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004356; F:glutamine synthetase activity; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0006542; P:glutamine biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.10.20.70; Glutamine synthetase, N-terminal domain; 1.
DR Gene3D; 3.30.590.10; Glutamine synthetase/guanido kinase, catalytic domain; 2.
DR InterPro; IPR008147; Gln_synt_N.
DR InterPro; IPR036651; Gln_synt_N_sf.
DR InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR InterPro; IPR008146; Gln_synth_cat_dom.
DR InterPro; IPR027303; Gln_synth_gly_rich_site.
DR InterPro; IPR027302; Gln_synth_N_conserv_site.
DR PANTHER; PTHR20852; GLUTAMINE SYNTHETASE; 1.
DR PANTHER; PTHR20852:SF93; GLUTAMINE SYNTHETASE CYTOSOLIC ISOZYME 1-4; 1.
DR Pfam; PF00120; Gln-synt_C; 1.
DR Pfam; PF03951; Gln-synt_N; 1.
DR SMART; SM01230; Gln-synt_C; 1.
DR SUPFAM; SSF54368; Glutamine synthetase, N-terminal domain; 1.
DR SUPFAM; SSF55931; Glutamine synthetase/guanido kinase; 1.
DR PROSITE; PS00180; GLNA_1; 1.
DR PROSITE; PS00181; GLNA_ATP; 1.
DR PROSITE; PS51986; GS_BETA_GRASP; 1.
DR PROSITE; PS51987; GS_CATALYTIC; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cytoplasm; Ligase; Nitrogen fixation; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..356
FT /note="Glutamine synthetase cytosolic isozyme 2"
FT /id="PRO_0000153197"
FT DOMAIN 19..99
FT /note="GS beta-grasp"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01330"
FT DOMAIN 106..356
FT /note="GS catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01331"
SQ SEQUENCE 356 AA; 39208 MW; 4B9C63B7718656B5 CRC64;
MSLLSDLINL NLSDITDKVI AEYIWVGGSG MDMRSKARTL SGPVKDPSKL PKWNYDGSST
GQAPGQDSEV ILYPQAIFKD PFRRGSNILV MCDAYTPAGE PIPTNKRNNA AKIFGHPDVA
AEEPWYGLEQ EYTLLQKDVQ WPLGWPLGGF PGPQGPYYCG TGANKAFGRD IVDSHYKACI
YAGINISGIN GEVMPGQWEF QVGPSIGISA ADELWVARYI LERITEIAGV VLSFDPKPIQ
GDWNGAGAHT NYSTKSMRND GGYEVIKKAI AKLEKRHKEH IAAYGEGNER RLTGRHETAD
MNTFVWGVAN RGASIRVGRD TEKAGKGYFE DRRPASNMDP YVVTSMIAET TILWKP
//
