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Database: UniProt
Entry: O83258
LinkDB: O83258
Original site: O83258 
ID   PRIA_TREPA              Reviewed;         657 AA.
AC   O83258;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   16-OCT-2019, entry version 114.
DE   RecName: Full=Primosomal protein N' {ECO:0000255|HAMAP-Rule:MF_00983};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_00983};
DE   AltName: Full=ATP-dependent helicase PriA {ECO:0000255|HAMAP-Rule:MF_00983};
GN   Name=priA {ECO:0000255|HAMAP-Rule:MF_00983};
GN   OrderedLocusNames=TP_0230;
OS   Treponema pallidum (strain Nichols).
OC   Bacteria; Spirochaetes; Spirochaetales; Spirochaetaceae; Treponema.
OX   NCBI_TaxID=243276;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Nichols;
RX   PubMed=9665876; DOI=10.1126/science.281.5375.375;
RA   Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G.,
RA   Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A.,
RA   Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D.,
RA   Khalak H.G., Richardson D.L., Howell J.K., Chidambaram M.,
RA   Utterback T.R., McDonald L.A., Artiach P., Bowman C., Cotton M.D.,
RA   Fujii C., Garland S.A., Hatch B., Horst K., Roberts K.M., Sandusky M.,
RA   Weidman J.F., Smith H.O., Venter J.C.;
RT   "Complete genome sequence of Treponema pallidum, the syphilis
RT   spirochete.";
RL   Science 281:375-388(1998).
CC   -!- FUNCTION: Involved in the restart of stalled replication forks.
CC       Recognizes and binds the arrested nascent DNA chain at stalled
CC       replication forks. It can open the DNA duplex, via its helicase
CC       activity, and promote assembly of the primosome and loading of the
CC       major replicative helicase DnaB onto DNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00983}.
CC   -!- SUBUNIT: Component of the primosome. {ECO:0000255|HAMAP-
CC       Rule:MF_00983}.
CC   -!- SIMILARITY: Belongs to the helicase family. PriA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00983}.
DR   EMBL; AE000520; AAC65217.1; -; Genomic_DNA.
DR   PIR; D71351; D71351.
DR   RefSeq; WP_010881678.1; NC_021490.2.
DR   SMR; O83258; -.
DR   STRING; 243276.TPANIC_0230; -.
DR   EnsemblBacteria; AAC65217; AAC65217; TP_0230.
DR   KEGG; tpa:TP_0230; -.
DR   eggNOG; ENOG4105C25; Bacteria.
DR   eggNOG; COG1198; LUCA.
DR   KO; K04066; -.
DR   OMA; RCHYCGY; -.
DR   OrthoDB; 1132322at2; -.
DR   Proteomes; UP000000811; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.8.630; -; 1.
DR   HAMAP; MF_00983; PriA; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005259; PriA.
DR   InterPro; IPR041222; PriA_3primeBD.
DR   InterPro; IPR042115; PriA_3primeBD_sf.
DR   InterPro; IPR041236; PriA_C.
DR   InterPro; IPR040498; PriA_CRR.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF17764; PriA_3primeBD; 1.
DR   Pfam; PF18074; PriA_C; 1.
DR   Pfam; PF18319; PriA_CRR; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00595; priA; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; DNA replication; DNA-binding;
KW   Helicase; Hydrolase; Metal-binding; Nucleotide-binding; Primosome;
KW   Reference proteome; Zinc; Zinc-finger.
FT   CHAIN         1    657       Primosomal protein N'.
FT                                /FTId=PRO_0000102136.
FT   DOMAIN      143    309       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00983}.
FT   DOMAIN      390    570       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_00983}.
FT   NP_BIND     156    163       ATP. {ECO:0000255|HAMAP-Rule:MF_00983}.
FT   ZN_FING     366    378       C4-type. {ECO:0000255|HAMAP-
FT                                Rule:MF_00983}.
FT   ZN_FING     393    409       C4-type. {ECO:0000255|HAMAP-
FT                                Rule:MF_00983}.
FT   MOTIF       252    255       DEAH box.
SQ   SEQUENCE   657 AA;  72168 MW;  64D8423F390577DD CRC64;
     MAPWLELVFD VPLDKSFTYR ACAAHAGEAL VGRRVLAPFG ARTLIGFVIS ESHSSPADCG
     GAVGTFKEII RVIDREALFD QTHLACARWM AHFYLCALGQ ALCAVVPSRK RERTLSSFAS
     CAGVRRTDTY ALSGEQRKAI DAITASTGAR SFYVHGVTGS GKTEVFLRAA EAVLARGKSV
     IYLVPEIALT HQVLQEVYVR FGSQAAVLHS ALSGSQRLGE WRRIQCMRHC VVIGARSAIF
     APLKRLGLVI MDEEHDSSYK SAHVPRYHAR QVAMYRCADA NCPFVMGSAT PSVEAWYAML
     RGAVRRLPLT ARVAGGAPPR VEVVDVSKEA LLLSTRLVDE IRKTKEAGYQ SMLFLNRRGF
     SYSFQCRSCG YTLCCTQCAV PLTWHKRVGA MQCHYCGRQE APPESCPCCH SFDTRYGGVG
     TEYIEEAVQA LFPEYRIARV DTDALRSGHV QQTMEQFRAG KIDVLLGTQM IAKGFNFPTL
     RLVGIACADT GLHTPDFRAA ERSFALMMQV AGRAGRYVDN GLVIIQTRNP AHPAVVCAQH
     GDCESFYAQE LAQREALCFP PFVRLIRFVF RSKTRRKAKD AAYAAHALLT AQMPLGADVL
     GPAACVVAQV AGSYRMQILL RAPSFPVVQQ VARSFLDEFR APAGVYVESD VDPVNVL
//
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