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Database: UniProt
Entry: P04047
LinkDB: P04047
Original site: P04047 
ID   KITH_CHICK              Reviewed;         224 AA.
AC   P04047;
DT   01-NOV-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1986, sequence version 1.
DT   13-FEB-2019, entry version 117.
DE   RecName: Full=Thymidine kinase, cytosolic;
DE            EC=2.7.1.21;
GN   Name=TK1;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes;
OC   Phasianidae; Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6328447; DOI=10.1093/nar/12.9.3959;
RA   Kwoh T.J., Engler J.A.;
RT   "The nucleotide sequence of the chicken thymidine kinase gene and the
RT   relationship of its predicted polypeptide to that of the vaccinia
RT   virus thymidine kinase.";
RL   Nucleic Acids Res. 12:3959-3971(1984).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6092937; DOI=10.1128/MCB.4.9.1769;
RA   Merrill G.F., Harland R.M., Groudine M., McKnight S.L.;
RT   "Genetic and physical analysis of the chicken tk gene.";
RL   Mol. Cell. Biol. 4:1769-1776(1984).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + thymidine = ADP + dTMP + H(+);
CC         Xref=Rhea:RHEA:19129, ChEBI:CHEBI:15378, ChEBI:CHEBI:17748,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:63528, ChEBI:CHEBI:456216;
CC         EC=2.7.1.21;
CC   -!- INTERACTION:
CC       Self; NbExp=2; IntAct=EBI-8306403, EBI-8306403;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MISCELLANEOUS: Two forms have been identified in animal cells, one
CC       in cytosol and one in mitochondria. Activity of the cytosolic
CC       enzyme is high in proliferating cells and peaks during the S-phase
CC       of the cell cycle; it is very low in resting cells.
CC   -!- SIMILARITY: Belongs to the thymidine kinase family. {ECO:0000305}.
DR   EMBL; K02611; AAA49096.1; -; Genomic_DNA.
DR   PIR; A00608; KICHT.
DR   RefSeq; NP_990229.1; NM_204898.1.
DR   UniGene; Gga.421; -.
DR   ProteinModelPortal; P04047; -.
DR   SMR; P04047; -.
DR   MINT; P04047; -.
DR   STRING; 9031.ENSGALP00000011630; -.
DR   PaxDb; P04047; -.
DR   PRIDE; P04047; -.
DR   Ensembl; ENSGALT00000082171; ENSGALP00000053115; ENSGALG00000007191.
DR   GeneID; 395719; -.
DR   KEGG; gga:395719; -.
DR   CTD; 7083; -.
DR   eggNOG; KOG3125; Eukaryota.
DR   eggNOG; COG1435; LUCA.
DR   GeneTree; ENSGT00390000011309; -.
DR   HOGENOM; HOG000076390; -.
DR   HOVERGEN; HBG006215; -.
DR   InParanoid; P04047; -.
DR   KO; K00857; -.
DR   OMA; CAGLDQD; -.
DR   OrthoDB; 1413914at2759; -.
DR   PhylomeDB; P04047; -.
DR   TreeFam; TF314839; -.
DR   BRENDA; 2.7.1.21; 1306.
DR   Reactome; R-GGA-73614; Pyrimidine salvage.
DR   PRO; PR:P04047; -.
DR   Proteomes; UP000000539; Chromosome 18.
DR   Bgee; ENSGALG00000007191; Expressed in 9 organ(s), highest expression level in colon.
DR   ExpressionAtlas; P04047; baseline.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0004797; F:thymidine kinase activity; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; ISS:UniProtKB.
DR   GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0006259; P:DNA metabolic process; IBA:GO_Central.
DR   GO; GO:0051289; P:protein homotetramerization; IEA:Ensembl.
DR   GO; GO:0046104; P:thymidine metabolic process; IBA:GO_Central.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001267; Thymidine_kinase.
DR   InterPro; IPR020633; Thymidine_kinase_CS.
DR   PANTHER; PTHR11441; PTHR11441; 1.
DR   Pfam; PF00265; TK; 1.
DR   PIRSF; PIRSF035805; TK_cell; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00603; TK_CELLULAR_TYPE; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Complete proteome; Cytoplasm; DNA synthesis; Kinase;
KW   Metal-binding; Nucleotide-binding; Reference proteome; Transferase;
KW   Zinc.
FT   CHAIN         1    224       Thymidine kinase, cytosolic.
FT                                /FTId=PRO_0000174946.
FT   NP_BIND      26     33       ATP. {ECO:0000305}.
FT   NP_BIND      58     60       ATP. {ECO:0000250}.
FT   NP_BIND      98    101       ATP. {ECO:0000250}.
FT   REGION      173    177       Substrate binding. {ECO:0000250}.
FT   ACT_SITE     99     99       Proton acceptor. {ECO:0000255}.
FT   METAL       154    154       Zinc. {ECO:0000250}.
FT   METAL       157    157       Zinc. {ECO:0000250}.
FT   METAL       186    186       Zinc. {ECO:0000250}.
FT   METAL       189    189       Zinc. {ECO:0000250}.
FT   BINDING     129    129       Substrate; via amide nitrogen.
FT                                {ECO:0000250}.
FT   BINDING     182    182       Substrate. {ECO:0000250}.
SQ   SEQUENCE   224 AA;  24872 MW;  BF29BEED2892BCF6 CRC64;
     MNCLTVPGVH PGSPGRPRGQ IQVIFGPMFS GKSTELMRRV RRFQLAQYRC LLVKYAKDTR
     YCTTGVSTHD RNTMEARPAC ALQDVYQEAL GSAVIGIDEG QFFPDIVEFC EKMANTGKTV
     IVAALDGTFQ RKAFGSILNL VPLAESVVKL NAVCMECYRE ASYTKRLGAE REVEVIGGAD
     KYHSVCRACY FQKRPQQLGS ENKENVPMGV KQLDMPASRK IFAS
//
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