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Database: UniProt
Entry: P04364
LinkDB: P04364
Original site: P04364 
ID   KITH_VAR67              Reviewed;         177 AA.
AC   P04364; Q9QNJ1;
DT   20-MAR-1987, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 2.
DT   16-JAN-2019, entry version 96.
DE   RecName: Full=Thymidine kinase;
DE            EC=2.7.1.21;
GN   Name=TK; ORFNames=J2R, L2R, M2R;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; dsDNA viruses, no RNA stage; Poxviridae; Chordopoxvirinae;
OC   Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8383392; DOI=10.1016/0168-1702(93)90110-9;
RA   Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S.,
RA   Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V.,
RA   Gashikov P.V., Belanov E.F., Belavin P.A., Resenchuk S.M.,
RA   Andzhaparidze O.G., Sandakhchiev L.S.;
RT   "Nucleotide sequence analysis of variola virus HindIII M, L, I genome
RT   fragments.";
RL   Virus Res. 27:25-35(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-R;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Bangladesh-1975;
RX   PubMed=6330986; DOI=10.1016/0042-6822(84)90212-5;
RA   Esposito J.J., Knight J.C.;
RT   "Nucleotide sequence of the thymidine kinase gene region of monkeypox
RT   and variola viruses.";
RL   Virology 135:561-567(1984).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Bangladesh-1975;
RX   PubMed=8264798; DOI=10.1038/366748a0;
RA   Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R.,
RA   Knight J.C., Aubin L., Yuran T.E., Parsons J.M., Loparev V.N.,
RA   Selivanov N.A., Cavallaro K.F., Kerlavage A.R., Mahy B.W.J.,
RA   Venter J.C.;
RT   "Potential virulence determinants in terminal regions of variola
RT   smallpox virus genome.";
RL   Nature 366:748-751(1993).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Garcia-1966;
RX   PubMed=10639322; DOI=10.1006/viro.1999.0086;
RA   Shchelkunov S.N., Totmenin A.V., Loparev V.N., Safronov P.F.,
RA   Gutorov V.V., Chizhikov V.E., Knight J.C., Parsons J.M., Massung R.F.,
RA   Esposito J.J.;
RT   "Alastrim smallpox variola minor virus genome DNA sequences.";
RL   Virology 266:361-386(2000).
CC   -!- FUNCTION: Phosphorylates thymidine and thymidine analogs, such as
CC       azidothymidine (AZT). Part of the salvage pathway for pyrimidine
CC       deoxyribonucleotide synthesis (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + thymidine = ADP + dTMP + H(+);
CC         Xref=Rhea:RHEA:19129, ChEBI:CHEBI:15378, ChEBI:CHEBI:17748,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:63528, ChEBI:CHEBI:456216;
CC         EC=2.7.1.21;
CC   -!- SUBUNIT: Homotetramer. Two molecules of substrate bind to each
CC       enzyme tetramer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the thymidine kinase family. {ECO:0000305}.
DR   EMBL; X67119; CAA47578.1; -; Genomic_DNA.
DR   EMBL; K02031; AAA80461.1; -; Genomic_DNA.
DR   EMBL; S55844; AAB24675.1; -; Genomic_DNA.
DR   EMBL; X69198; CAA49020.1; -; Genomic_DNA.
DR   EMBL; L22579; AAA60827.1; -; Genomic_DNA.
DR   EMBL; Y16780; CAB54679.1; -; Genomic_DNA.
DR   PIR; A00611; KIVZVV.
DR   PIR; E72160; E72160.
DR   PIR; T28517; T28517.
DR   RefSeq; NP_042123.1; NC_001611.1.
DR   ProteinModelPortal; P04364; -.
DR   PRIDE; P04364; -.
DR   GeneID; 1486465; -.
DR   KEGG; vg:1486465; -.
DR   Proteomes; UP000002060; Genome.
DR   Proteomes; UP000111493; Genome.
DR   Proteomes; UP000119805; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004797; F:thymidine kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001267; Thymidine_kinase.
DR   InterPro; IPR020633; Thymidine_kinase_CS.
DR   PANTHER; PTHR11441; PTHR11441; 1.
DR   Pfam; PF00265; TK; 1.
DR   PIRSF; PIRSF035805; TK_cell; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00603; TK_CELLULAR_TYPE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; DNA synthesis; Kinase; Metal-binding;
KW   Nucleotide-binding; Reference proteome; Transferase; Zinc.
FT   CHAIN         1    177       Thymidine kinase.
FT                                /FTId=PRO_0000174941.
FT   NP_BIND      11     18       ATP. {ECO:0000250}.
FT   REGION      157    161       Substrate binding. {ECO:0000250}.
FT   ACT_SITE     83     83       Proton acceptor. {ECO:0000255}.
FT   METAL       138    138       Zinc. {ECO:0000250}.
FT   METAL       141    141       Zinc. {ECO:0000250}.
FT   METAL       170    170       Zinc. {ECO:0000250}.
FT   METAL       173    173       Zinc. {ECO:0000250}.
FT   BINDING     113    113       Substrate; via amide nitrogen.
FT                                {ECO:0000250}.
FT   VARIANT      90     90       I -> V (in strain: Bangladesh-1975).
FT   VARIANT     153    153       T -> A (in strain: Garcia-1966).
FT   VARIANT     163    163       I -> N (in strain: Bangladesh-1975).
SQ   SEQUENCE   177 AA;  20055 MW;  580FF33308BE5A35 CRC64;
     MNGGHIQLII GPMFSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL WTHDKNNFEA
     LEATKLCDVL EAITDFSVIG IDEGQFFPDI VEFCERMANE GKIVIVAALD GTFQRKPFNN
     ILDLIPLSEM VVKLTAVCMK CFKEASFSKR LGTETKIEII GGIDMYQSVC RKCYIDS
//
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