ID NLPA_ECOLI Reviewed; 272 AA.
AC P04846; Q2M7X3;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 24-JAN-2024, entry version 164.
DE RecName: Full=Lipoprotein 28;
DE Flags: Precursor;
GN Name=nlpA; OrderedLocusNames=b3661, JW3635;
OS Escherichia coli (strain K12).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DIACYLGLYCEROL AT CYS-24.
RX PubMed=3003106; DOI=10.1016/s0021-9258(17)35931-8;
RA Yu F., Inouye S., Inouye M.;
RT "Lipoprotein-28, a cytoplasmic membrane lipoprotein from Escherichia coli.
RT Cloning, DNA sequence, and expression of its gene.";
RL J. Biol. Chem. 261:2284-2288(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7686882; DOI=10.1006/geno.1993.1230;
RA Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.;
RT "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome:
RT organizational symmetry around the origin of replication.";
RL Genomics 16:551-561(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Lipid-anchor.
CC -!- SIMILARITY: Belongs to the NlpA lipoprotein family. {ECO:0000305}.
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DR EMBL; M12163; AAA24080.1; -; Genomic_DNA.
DR EMBL; L10328; AAA62013.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76684.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77633.1; -; Genomic_DNA.
DR PIR; A26286; LPEC28.
DR RefSeq; NP_418117.1; NC_000913.3.
DR RefSeq; WP_000779426.1; NZ_SSZK01000043.1.
DR AlphaFoldDB; P04846; -.
DR SMR; P04846; -.
DR BioGRID; 4259626; 12.
DR BioGRID; 852481; 1.
DR IntAct; P04846; 8.
DR STRING; 511145.b3661; -.
DR SWISS-2DPAGE; P04846; -.
DR jPOST; P04846; -.
DR PaxDb; 511145-b3661; -.
DR EnsemblBacteria; AAC76684; AAC76684; b3661.
DR GeneID; 66672443; -.
DR GeneID; 948175; -.
DR KEGG; ecj:JW3635; -.
DR KEGG; eco:b3661; -.
DR PATRIC; fig|1411691.4.peg.3045; -.
DR EchoBASE; EB0651; -.
DR eggNOG; COG1464; Bacteria.
DR HOGENOM; CLU_067080_0_0_6; -.
DR InParanoid; P04846; -.
DR OMA; QDNKAHN; -.
DR OrthoDB; 9812878at2; -.
DR PhylomeDB; P04846; -.
DR BioCyc; EcoCyc:EG10657-MONOMER; -.
DR PRO; PR:P04846; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR CDD; cd13598; PBP2_lipoprotein_IlpA_like; 1.
DR Gene3D; 3.40.190.10; Periplasmic binding protein-like II; 2.
DR InterPro; IPR004872; Lipoprotein_NlpA.
DR NCBIfam; TIGR00363; MetQ/NlpA family lipoprotein; 1.
DR PANTHER; PTHR30429; D-METHIONINE-BINDING LIPOPROTEIN METQ; 1.
DR PANTHER; PTHR30429:SF1; D-METHIONINE-BINDING LIPOPROTEIN METQ-RELATED; 1.
DR Pfam; PF03180; Lipoprotein_9; 1.
DR PIRSF; PIRSF002854; MetQ; 1.
DR SUPFAM; SSF53850; Periplasmic binding protein-like II; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Lipoprotein; Membrane; Palmitate;
KW Reference proteome; Signal.
FT SIGNAL 1..23
FT CHAIN 24..272
FT /note="Lipoprotein 28"
FT /id="PRO_0000019737"
FT LIPID 24
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000305"
FT LIPID 24
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303,
FT ECO:0000269|PubMed:3003106"
SQ SEQUENCE 272 AA; 29424 MW; E3C249E753AB1B33 CRC64;
MKLTTHHLRT GAALLLAGIL LAGCDQSSSD AKHIKVGVIN GAEQDVAEVA KKVAKEKYGL
DVELVGFSGS LLPNDATNHG ELDANVFQHR PFLEQDNQAH GYKLVAVGNT FVFPMAGYSK
KIKTVAQIKE GATVAIPNDP TNLGRALLLL QKEKLITLKE GKGLLPTALD ITDNPRHLQI
MELEGAQLPR VLDDPKVDVA IISTTYIQQT GLSPVHDSVF IEDKNSPYVN ILVAREDNKN
AENVKEFLQS YQSPEVAKAA ETIFNGGAVP GW
//
