ID DNAA_BACSU Reviewed; 446 AA.
AC P05648;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1988, sequence version 1.
DT 27-MAR-2024, entry version 168.
DE RecName: Full=Chromosomal replication initiator protein DnaA;
GN Name=dnaA; Synonyms=dnaH; OrderedLocusNames=BSU00010;
OS Bacillus subtilis (strain 168).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2987847; DOI=10.1093/nar/13.7.2251;
RA Moriya S., Ogasawara N., Yoshikawa H.;
RT "Structure and function of the region of the replication origin of the
RT Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base
RT pairs in the origin region.";
RL Nucleic Acids Res. 13:2251-2265(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA Ogasawara N., Nakai S., Yoshikawa H.;
RT "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis
RT chromosome containing the replication origin.";
RL DNA Res. 1:1-14(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6 AND 441-446.
RC STRAIN=168 / PSL1;
RX PubMed=2846289; DOI=10.1002/j.1460-2075.1988.tb03149.x;
RA Moriya S., Fukuoka T., Ogasawara N., Yoshikawa H.;
RT "Regulation of initiation of the chromosomal replication by DnaA-boxes in
RT the origin region of the Bacillus subtilis chromosome.";
RL EMBO J. 7:2911-2917(1988).
RN [5]
RP INTERACTION WITH CCRZ.
RC STRAIN=168 / PY79 {ECO:0000303|PubMed:35576203};
RX PubMed=35576203; DOI=10.1371/journal.pgen.1010196;
RA Wozniak K.J., Burby P.E., Nandakumar J., Simmons L.A.;
RT "Structure and kinase activity of bacterial cell cycle regulator CcrZ.";
RL PLoS Genet. 18:e1010196-e1010196(2022).
CC -!- FUNCTION: Plays an important role in the initiation and regulation of
CC chromosomal replication. Binds to the origin of replication; it binds
CC specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-
CC TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids. DnaA
CC can inhibit its own gene expression as well as that of other genes.
CC -!- SUBUNIT: Interacts (via domains I (1-82) and III (111-326)) with CcrZ
CC (PubMed:35576203). Some 20 DnaA protein molecules bind their sites in
CC oriC. {ECO:0000269|PubMed:35576203}.
CC -!- INTERACTION:
CC P05648; P05648: dnaA; NbExp=2; IntAct=EBI-2014722, EBI-2014722;
CC P05648; P37522: soj; NbExp=2; IntAct=EBI-2014722, EBI-2014674;
CC P05648; P37542: yabA; NbExp=4; IntAct=EBI-2014722, EBI-5243764;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DnaA family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X02369; CAA26217.1; -; Genomic_DNA.
DR EMBL; D26185; BAA05237.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB11777.1; -; Genomic_DNA.
DR EMBL; X12778; CAA31269.1; -; Genomic_DNA.
DR EMBL; X12779; CAA31270.1; -; Genomic_DNA.
DR PIR; A22930; IQBSOC.
DR RefSeq; NP_387882.1; NC_000964.3.
DR RefSeq; WP_003242674.1; NZ_JNCM01000034.1.
DR PDB; 4TPS; X-ray; 1.65 A; B/D=1-82.
DR PDBsum; 4TPS; -.
DR AlphaFoldDB; P05648; -.
DR SMR; P05648; -.
DR DIP; DIP-41940N; -.
DR IntAct; P05648; 7.
DR STRING; 224308.BSU00010; -.
DR PaxDb; 224308-BSU00010; -.
DR EnsemblBacteria; CAB11777; CAB11777; BSU_00010.
DR GeneID; 83883200; -.
DR GeneID; 939978; -.
DR KEGG; bsu:BSU00010; -.
DR PATRIC; fig|224308.179.peg.1; -.
DR eggNOG; COG0593; Bacteria.
DR InParanoid; P05648; -.
DR OrthoDB; 9807019at2; -.
DR PhylomeDB; P05648; -.
DR BioCyc; BSUB:BSU00010-MONOMER; -.
DR PRO; PR:P05648; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:1990101; C:DnaA-oriC complex; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR GO; GO:0006270; P:DNA replication initiation; IDA:CACAO.
DR GO; GO:0006275; P:regulation of DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd00009; AAA; 1.
DR CDD; cd06571; Bac_DnaA_C; 1.
DR Gene3D; 1.10.1750.10; -; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 3.30.300.180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_00377; DnaA_bact; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR001957; Chromosome_initiator_DnaA.
DR InterPro; IPR020591; Chromosome_initiator_DnaA-like.
DR InterPro; IPR018312; Chromosome_initiator_DnaA_CS.
DR InterPro; IPR013317; DnaA.
DR InterPro; IPR013159; DnaA_C.
DR InterPro; IPR024633; DnaA_N_dom.
DR InterPro; IPR038454; DnaA_N_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR010921; Trp_repressor/repl_initiator.
DR NCBIfam; TIGR00362; DnaA; 1.
DR PANTHER; PTHR30050; CHROMOSOMAL REPLICATION INITIATOR PROTEIN DNAA; 1.
DR PANTHER; PTHR30050:SF2; CHROMOSOMAL REPLICATION INITIATOR PROTEIN DNAA; 1.
DR Pfam; PF00308; Bac_DnaA; 1.
DR Pfam; PF08299; Bac_DnaA_C; 1.
DR Pfam; PF11638; DnaA_N; 1.
DR PRINTS; PR00051; DNAA.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00760; Bac_DnaA_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF48295; TrpR-like; 1.
DR PROSITE; PS01008; DNAA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cytoplasm; DNA replication; DNA-binding;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..446
FT /note="Chromosomal replication initiator protein DnaA"
FT /id="PRO_0000114133"
FT BINDING 151..158
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT HELIX 1..16
FT /evidence="ECO:0007829|PDB:4TPS"
FT HELIX 21..27
FT /evidence="ECO:0007829|PDB:4TPS"
FT TURN 28..30
FT /evidence="ECO:0007829|PDB:4TPS"
FT STRAND 32..37
FT /evidence="ECO:0007829|PDB:4TPS"
FT STRAND 40..44
FT /evidence="ECO:0007829|PDB:4TPS"
FT HELIX 48..57
FT /evidence="ECO:0007829|PDB:4TPS"
FT HELIX 59..70
FT /evidence="ECO:0007829|PDB:4TPS"
FT STRAND 75..79
FT /evidence="ECO:0007829|PDB:4TPS"
SQ SEQUENCE 446 AA; 50859 MW; 666799A0A183C992 CRC64;
MENILDLWNQ ALAQIEKKLS KPSFETWMKS TKAHSLQGDT LTITAPNEFA RDWLESRYLH
LIADTIYELT GEELSIKFVI PQNQDVEDFM PKPQVKKAVK EDTSDFPQNM LNPKYTFDTF
VIGSGNRFAH AASLAVAEAP AKAYNPLFIY GGVGLGKTHL MHAIGHYVID HNPSAKVVYL
SSEKFTNEFI NSIRDNKAVD FRNRYRNVDV LLIDDIQFLA GKEQTQEEFF HTFNTLHEES
KQIVISSDRP PKEIPTLEDR LRSRFEWGLI TDITPPDLET RIAILRKKAK AEGLDIPNEV
MLYIANQIDS NIRELEGALI RVVAYSSLIN KDINADLAAE ALKDIIPSSK PKVITIKEIQ
RVVGQQFNIK LEDFKAKKRT KSVAFPRQIA MYLSREMTDS SLPKIGEEFG GRDHTTVIHA
HEKISKLLAD DEQLQQHVKE IKEQLK
//
