ID GSB_DROME Reviewed; 427 AA.
AC P09082; Q9W0W4;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1988, sequence version 1.
DT 24-JAN-2024, entry version 200.
DE RecName: Full=Protein gooseberry;
DE AltName: Full=BSH9;
DE AltName: Full=Protein gooseberry distal;
GN Name=gsb; Synonyms=GSB-D, GSBB; ORFNames=CG3388;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE, FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=3123319; DOI=10.1101/gad.1.10.1247;
RA Baumgartner S., Bopp D., Burri M., Noll M.;
RT "Structure of two genes at the gooseberry locus related to the paired gene
RT and their spatial expression during Drosophila embryogenesis.";
RL Genes Dev. 1:1247-1267(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 9-148 AND 165-244.
RX PubMed=2877747; DOI=10.1016/0092-8674(86)90818-4;
RA Bopp D., Burri M., Baumgartner S., Frigerio G., Noll M.;
RT "Conservation of a large protein domain in the segmentation gene paired and
RT in functionally related genes of Drosophila.";
RL Cell 47:1033-1040(1986).
CC -!- FUNCTION: Expressed in segmentally repeating pattern to define the
CC polarity of embryonic segments. {ECO:0000269|PubMed:3123319}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Expressed in the posterior ventrolateral part of
CC each segment throughout the embryo, including head and tail segments.
CC At first expression is restricted to the ectoderm. During germ-band
CC extension, expression is induced in the mesoderm.
CC {ECO:0000269|PubMed:3123319}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000305}.
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DR EMBL; M14944; AAA28836.1; -; mRNA.
DR EMBL; M14942; AAA28837.1; -; mRNA.
DR EMBL; AE013599; AAF47315.2; -; Genomic_DNA.
DR PIR; A43698; A43698.
DR RefSeq; NP_523863.1; NM_079139.4.
DR AlphaFoldDB; P09082; -.
DR SMR; P09082; -.
DR BioGRID; 63573; 13.
DR DIP; DIP-18421N; -.
DR IntAct; P09082; 4.
DR STRING; 7227.FBpp0072349; -.
DR PaxDb; 7227-FBpp0072349; -.
DR DNASU; 38005; -.
DR EnsemblMetazoa; FBtr0072444; FBpp0072349; FBgn0001148.
DR GeneID; 38005; -.
DR KEGG; dme:Dmel_CG3388; -.
DR AGR; FB:FBgn0001148; -.
DR CTD; 38005; -.
DR FlyBase; FBgn0001148; gsb.
DR VEuPathDB; VectorBase:FBgn0001148; -.
DR eggNOG; KOG0849; Eukaryota.
DR GeneTree; ENSGT00940000168362; -.
DR HOGENOM; CLU_019281_0_0_1; -.
DR InParanoid; P09082; -.
DR OMA; SWDSYNF; -.
DR OrthoDB; 5398393at2759; -.
DR PhylomeDB; P09082; -.
DR Reactome; R-DME-3214847; HATs acetylate histones.
DR SignaLink; P09082; -.
DR BioGRID-ORCS; 38005; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 38005; -.
DR PRO; PR:P09082; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0001148; Expressed in ectoderm anlage and 29 other cell types or tissues.
DR ExpressionAtlas; P09082; baseline and differential.
DR Genevisible; P09082; DM.
DR GO; GO:0005737; C:cytoplasm; IMP:FlyBase.
DR GO; GO:0005634; C:nucleus; IMP:FlyBase.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IDA:FlyBase.
DR GO; GO:0022008; P:neurogenesis; IMP:FlyBase.
DR GO; GO:0060025; P:regulation of synaptic activity; IDA:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007435; P:salivary gland morphogenesis; IMP:FlyBase.
DR GO; GO:0007367; P:segment polarity determination; IGI:FlyBase.
DR GO; GO:0007419; P:ventral cord development; TAS:FlyBase.
DR CDD; cd00086; homeodomain; 1.
DR CDD; cd00131; PAX; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR000047; HTH_motif.
DR InterPro; IPR043182; PAIRED_DNA-bd_dom.
DR InterPro; IPR001523; Paired_dom.
DR InterPro; IPR043565; PAX_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR45636; PAIRED BOX PROTEIN PAX-6-RELATED-RELATED; 1.
DR PANTHER; PTHR45636:SF38; PROTEIN GOOSEBERRY-RELATED; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00292; PAX; 1.
DR PRINTS; PR00031; HTHREPRESSR.
DR PRINTS; PR00027; PAIREDBOX.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00351; PAX; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 2.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00034; PAIRED_1; 1.
DR PROSITE; PS51057; PAIRED_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Paired box;
KW Reference proteome; Segmentation polarity protein; Transcription;
KW Transcription regulation.
FT CHAIN 1..427
FT /note="Protein gooseberry"
FT /id="PRO_0000050168"
FT DNA_BIND 19..145
FT /note="Paired"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT DNA_BIND 185..244
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 22..78
FT /note="PAI subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 97..145
FT /note="RED subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 142..192
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 248..267
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 300..336
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 176..191
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 427 AA; 45515 MW; 04E22DF9B29F832B CRC64;
MAVSALNMTP YFGGYPFQGQ GRVNQLGGVF INGRPLPNHI RRQIVEMAAA GVRPCVISRQ
LRVSHGCVSK ILNRFQETGS IRPGVIGGSK PRVATPDIES RIEELKQSQP GIFSWEIRAK
LIEAGVCDKQ NAPSVSSISR LLRGSSGSGT SHSIDGILGG GAGSVGSEDE SEDDAEPSVQ
LKRKQRRSRT TFSNDQIDAL ERIFARTQYP DVYTREELAQ STGLTEARVQ VWFSNRRARL
RKQLNTQQVP SFAPTSTSFG ATPTTSAAPA PNMGMSLYSS QSWPSSGAYE NHAAYGGSVA
SMSPASSTSG TSSAAHSPVQ TQAQQPGTGS EFMTSTYGVG SSNATYPSAA YSMPQTPATS
AEQLRSQFAS AAASGSHHPS TWDSYNFAGS FFPPASAAGN HISGYHHQVD QKSSMMTTAP
TYPYFGF
//
