ID OMPR_SHIFL Reviewed; 239 AA.
AC P0AA21; O31133; P03025; P08981; P41405;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 27-MAR-2024, entry version 120.
DE RecName: Full=DNA-binding dual transcriptional regulator OmpR {ECO:0000305};
DE AltName: Full=Transcriptional regulatory protein OmpR {ECO:0000305};
GN Name=ompR; OrderedLocusNames=SF3424, S4339;
OS Shigella flexneri.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Weber A., Jung K.;
RT "Nucleotide sequence of the ompB operon of Shigella flexneri.";
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 48-239.
RC STRAIN=ATCC 12022 / CDC 3591-52 / Serotype 2b;
RX PubMed=10464234; DOI=10.1128/jb.181.17.5534-5538.1999;
RA Waukau J., Forst S.;
RT "Identification of a conserved N-terminal sequence involved in
RT transmembrane signal transduction in EnvZ.";
RL J. Bacteriol. 181:5534-5538(1999).
CC -!- FUNCTION: Member of the two-component regulatory system EnvZ/OmpR
CC involved in regulating expression of the outer membrane porins OmpC and
CC OmpF as well as other genes. Unlike E.coli, OmpC is expressed at both
CC low and high osmolarity, while OmpF is expressed at low osmolarity (By
CC similarity). This two-component system plays a role in virulence (By
CC similarity). {ECO:0000250|UniProtKB:A0A4P7TS68}.
CC -!- SUBUNIT: Monomer and multimer. {ECO:0000250|UniProtKB:P0AA16}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P0AA16}.
CC -!- PTM: Phosphorylated by EnvZ; this stimulates its DNA-binding ability.
CC Asp-55 is the primary phosphate acceptor site.
CC {ECO:0000250|UniProtKB:P0AA16}.
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DR EMBL; AJ288905; CAC34268.1; -; Genomic_DNA.
DR EMBL; AE005674; AAN44885.2; -; Genomic_DNA.
DR EMBL; AE014073; AAP19294.1; -; Genomic_DNA.
DR EMBL; AF030314; AAB84276.1; -; Genomic_DNA.
DR RefSeq; NP_709178.2; NC_004337.2.
DR RefSeq; WP_001157751.1; NZ_WPGW01000003.1.
DR AlphaFoldDB; P0AA21; -.
DR SMR; P0AA21; -.
DR STRING; 198214.SF3424; -.
DR PaxDb; 198214-SF3424; -.
DR GeneID; 1025367; -.
DR GeneID; 8912757; -.
DR KEGG; sfl:SF3424; -.
DR KEGG; sfx:S4339; -.
DR PATRIC; fig|198214.7.peg.4040; -.
DR HOGENOM; CLU_000445_30_4_6; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 3.40.50.2300; -; 1.
DR Gene3D; 6.10.250.690; -; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111:SF4; DNA-BINDING DUAL TRANSCRIPTIONAL REGULATOR OMPR; 1.
DR PANTHER; PTHR48111; REGULATOR OF RPOS; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; C-terminal effector domain of the bipartite response regulators; 1.
DR SUPFAM; SSF52172; CheY-like; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 3: Inferred from homology;
KW Activator; Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome;
KW Repressor; Stress response; Transcription; Transcription regulation;
KW Two-component regulatory system; Virulence.
FT CHAIN 1..239
FT /note="DNA-binding dual transcriptional regulator OmpR"
FT /id="PRO_0000081181"
FT DOMAIN 6..120
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 135..234
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 55
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000250|UniProtKB:P0AA16,
FT ECO:0000255|PROSITE-ProRule:PRU00169"
FT CONFLICT 163..164
FT /note="SG -> RR (in Ref. 4; AAB84276)"
FT /evidence="ECO:0000305"
FT CONFLICT 178..179
FT /note="EP -> DA (in Ref. 4; AAB84276)"
FT /evidence="ECO:0000305"
FT CONFLICT 209
FT /note="R -> A (in Ref. 4; AAB84276)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 239 AA; 27354 MW; 823CA7720E9A1D2A CRC64;
MQENYKILVV DDDMRLRALL ERYLTEQGFQ VRSVANAEQM DRLLTRESFH LMVLDLMLPG
EDGLSICRRL RSQSNPMPII MVTAKGEEVD RIVGLEIGAD DYIPKPFNPR ELLARIRAVL
RRQANELPGA PSQEEAVIAF GKFKLNLGTR EMFREDEPMP LTSGEFAVLK ALVSHPREPL
SRDKLMNLAR GREYSAMERS IDVQISRLRR MVEEDPAHPR YIQTVWGLGY VFVPDGSKA
//
