GenomeNet

Database: UniProt
Entry: P0CG28
LinkDB: P0CG28
Original site: P0CG28 
ID   RPOD_METAC              Reviewed;         266 AA.
AC   P0CG28;
DT   13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT   13-JUL-2010, sequence version 1.
DT   16-JAN-2019, entry version 37.
DE   RecName: Full=DNA-directed RNA polymerase subunit D;
DE            EC=2.7.7.6;
GN   Name=rpoD; OrderedLocusNames=MA_1111;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P.,
RA   FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A.,
RA   Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R.,
RA   Linton L., McEwan P., McKernan K., Talamas J., Tirrell A., Ye W.,
RA   Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M.,
RA   Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A.,
RA   Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K.,
RA   Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C.,
RA   Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T.,
RA   Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E.,
RA   Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic
RT   and physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription
CC       of DNA into RNA using the four ribonucleoside triphosphates as
CC       substrates. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:11128, Rhea:RHEA-
CC         COMP:11129, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557,
CC         ChEBI:CHEBI:83400; EC=2.7.7.6;
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the archaeal RpoD/eukaryotic RPB3 RNA
CC       polymerase subunit family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AE010299; Type=Frameshift; Positions=156; Evidence={ECO:0000305};
DR   EMBL; AE010299; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   ProteinModelPortal; P0CG28; -.
DR   SMR; P0CG28; -.
DR   InParanoid; P0CG28; -.
DR   PhylomeDB; P0CG28; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.170.120.12; -; 1.
DR   Gene3D; 3.30.1360.10; -; 1.
DR   HAMAP; MF_00320; RNApol_arch_D; 1.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR   InterPro; IPR001514; DNA-dir_RNA_pol_30-40kDasu_CS.
DR   InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR   InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR   InterPro; IPR036603; RBP11-like.
DR   InterPro; IPR022842; RNAP_D.
DR   InterPro; IPR036643; RNApol_insert_sf.
DR   Pfam; PF01000; RNA_pol_A_bac; 1.
DR   Pfam; PF01193; RNA_pol_L; 1.
DR   SMART; SM00662; RPOLD; 1.
DR   SUPFAM; SSF55257; SSF55257; 1.
DR   SUPFAM; SSF56553; SSF56553; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 1.
DR   PROSITE; PS51379; 4FE4S_FER_2; 2.
DR   PROSITE; PS00446; RNA_POL_D_30KD; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Complete proteome; DNA-directed RNA polymerase; Iron;
KW   Iron-sulfur; Metal-binding; Nucleotidyltransferase;
KW   Reference proteome; Transcription; Transferase.
FT   CHAIN         1    266       DNA-directed RNA polymerase subunit D.
FT                                /FTId=PRO_0000395338.
FT   METAL       183    183       Iron-sulfur (4Fe-4S). {ECO:0000250}.
FT   METAL       205    205       Iron-sulfur (4Fe-4S). {ECO:0000250}.
FT   METAL       208    208       Iron-sulfur (4Fe-4S). {ECO:0000250}.
FT   METAL       211    211       Iron-sulfur (4Fe-4S). {ECO:0000250}.
SQ   SEQUENCE   266 AA;  28749 MW;  6B36A24248365BA3 CRC64;
     MTMEVDILEL SDRSAKFVLS NVSTAFANGI RRAMVADVPT LAIEYVNLYD NTSVLYDEQL
     ALRLSLIPLA TDIETYLPQA ECDVCGGEGC PACEVSLTLS AEGPGTVYSR DLISSDPKIQ
     PADPNVPIVE LKKGQKLVLE AIAHMGYGRD SVKWQAGVAC GYKNMPVITI ENCDACGHCA
     AECPKGIISV EEAGAKIAEE DIMKCSICRL CEQVCDINAI KVDFYENSFV FTMESDGSYT
     AKDLVLNAAN VIKGKAEEML AILDQL
//
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