ID MBF1_CRYNB Reviewed; 150 AA.
AC P0CO31; Q55KQ9; Q5KAR7;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 24-JAN-2024, entry version 52.
DE RecName: Full=Multiprotein-bridging factor 1;
GN Name=MBF1; OrderedLocusNames=CNBJ2740;
OS Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS (Filobasidiella neoformans).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=283643;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B-3501A;
RX PubMed=15653466; DOI=10.1126/science.1103773;
RA Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT neoformans.";
RL Science 307:1321-1324(2005).
CC -!- FUNCTION: Transcriptional coactivator that stimulates GCN4-dependent
CC transcriptional activity by bridging the DNA-binding region of GCN4 and
CC TBP (SPT15), thereby recruiting TBP to GCN4-bound promoters. Involved
CC in induction of the ribosome quality control (RQC) pathway; a pathway
CC that degrades nascent peptide chains during problematic translation.
CC Required to prevent stalled ribosomes from frameshifting.
CC {ECO:0000250|UniProtKB:O14467}.
CC -!- SIMILARITY: Belongs to the MBF1 family. {ECO:0000305}.
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DR EMBL; AAEY01000050; EAL18351.1; -; Genomic_DNA.
DR RefSeq; XP_772998.1; XM_767905.1.
DR AlphaFoldDB; P0CO31; -.
DR SMR; P0CO31; -.
DR EnsemblFungi; AAW45939; AAW45939; CNJ00750.
DR GeneID; 4938617; -.
DR KEGG; cnb:CNBJ2740; -.
DR VEuPathDB; FungiDB:CNBJ2740; -.
DR HOGENOM; CLU_112609_0_1_1; -.
DR OrthoDB; 120438at2759; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR CDD; cd00093; HTH_XRE; 1.
DR Gene3D; 1.10.260.40; lambda repressor-like DNA-binding domains; 1.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013729; MBF1_N.
DR PANTHER; PTHR10245:SF15; ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1; 1.
DR PANTHER; PTHR10245; ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1 MULTIPROTEIN BRIDGING FACTOR 1; 1.
DR Pfam; PF01381; HTH_3; 1.
DR Pfam; PF08523; MBF1; 1.
DR SMART; SM00530; HTH_XRE; 1.
DR SUPFAM; SSF47413; lambda repressor-like DNA-binding domains; 1.
DR PROSITE; PS50943; HTH_CROC1; 1.
PE 3: Inferred from homology;
KW Activator; DNA-binding; Transcription; Transcription regulation.
FT CHAIN 1..150
FT /note="Multiprotein-bridging factor 1"
FT /id="PRO_0000410120"
FT DOMAIN 84..137
FT /note="HTH cro/C1-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT DNA_BIND 94..113
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT REGION 36..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 51..68
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 150 AA; 15938 MW; 8362FA6E1EDF18E0 CRC64;
MSDWDKPTVI GFRQQKPTVA KGSTLNAAQR AGLVISSESK GAGQSKGPAD HQRIAKLDRD
DAPKPPEKVS ADVGKAVATA RMAIKNAEGK SMTQKELATS VNAKPQDIAD LESGRAVPDQ
ALLGKLERKL NVKLRGAKNL IGTPLHPKKK
//
