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Database: UniProt
Entry: P0CQ70
LinkDB: P0CQ70
Original site: P0CQ70 
ID   IF4A_CRYNJ              Reviewed;         401 AA.
AC   P0CQ70; Q55YU1; Q5KN60;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   16-OCT-2019, entry version 46.
DE   RecName: Full=ATP-dependent RNA helicase eIF4A;
DE            EC=3.6.4.13;
DE   AltName: Full=Eukaryotic initiation factor 4A;
DE            Short=eIF-4A;
DE   AltName: Full=Translation initiation factor 1;
GN   Name=TIF1; Synonyms=TIF41; OrderedLocusNames=CNA07620;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 /
OS   ATCC MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina;
OC   Tremellomycetes; Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J.,
RA   D'Souza C.A., Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J.,
RA   Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I.,
RA   Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E.,
RA   Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L.,
RA   Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A.,
RA   Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R.,
RA   Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W.,
RA   Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen
RT   Cryptococcus neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: ATP-dependent RNA helicase which is a subunit of the
CC       eIF4F complex involved in cap recognition and is required for mRNA
CC       binding to ribosome. In the current model of translation
CC       initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR
CC       of mRNAs which is necessary to allow efficient binding of the
CC       small ribosomal subunit, and subsequent scanning for the initiator
CC       codon (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Component of the eIF4F complex, which composition varies
CC       with external and internal environmental conditions. It is
CC       composed of at least eIF4A, eIF4E and eIF4G (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. eIF4A
CC       subfamily. {ECO:0000305}.
DR   EMBL; AE017341; AAW41293.1; -; Genomic_DNA.
DR   RefSeq; XP_567112.1; XM_567112.1.
DR   SMR; P0CQ70; -.
DR   STRING; 5207.AAW41293; -.
DR   PaxDb; P0CQ70; -.
DR   PRIDE; P0CQ70; -.
DR   EnsemblFungi; AAW41293; AAW41293; CNA07620.
DR   GeneID; 3253907; -.
DR   KEGG; cne:CNA07620; -.
DR   EuPathDB; FungiDB:CNA07620; -.
DR   eggNOG; KOG0327; Eukaryota.
DR   eggNOG; COG0513; LUCA.
DR   InParanoid; P0CQ70; -.
DR   KO; K03257; -.
DR   OMA; RNPIRIL; -.
DR   OrthoDB; 726081at2759; -.
DR   Proteomes; UP000002149; Chromosome 1.
DR   GO; GO:0010494; C:cytoplasmic stress granule; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR   GO; GO:0002183; P:cytoplasmic translational initiation; IBA:GO_Central.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; Helicase; Hydrolase;
KW   Initiation factor; Nucleotide-binding; Protein biosynthesis;
KW   Reference proteome; RNA-binding.
FT   CHAIN         1    401       ATP-dependent RNA helicase eIF4A.
FT                                /FTId=PRO_0000232134.
FT   DOMAIN       59    229       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      240    401       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND      72     79       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF        28     56       Q motif.
FT   MOTIF       177    180       DEAD box.
SQ   SEQUENCE   401 AA;  45144 MW;  32D6418B1BBD7C68 CRC64;
     MSDTKGAAEQ GLQIDGDLIN SNWNEVVDNF DDMKLKGELL RGIYAYGFER PSAIQQRAIM
     PIVTGRDCIA QAQSGTGKTA TFSVSILQRI DTTVKKTQAL VLAPTRELAQ QIQKVVIALG
     DYLNVDCHAC VGGTAVREDI ARLNEGPHIV VGTPGRVFDM INRGALKTEA VMMFCLDEAD
     EMLSTGFKES IYEIFQLLPG ETQVVLLSAT MAPEVLDVTK KFMRDPIRIL VKKDELTLEG
     IRQFYINVEK EEWKLETLCD LYETVTITQA VIFCSTRRKV DWLTQQLHDR QFTVSAMHGD
     MKQEEREVIM KEFRSGSSRV LITTDLLARG IDVQQVSLVI NYDLPSSKEN YIHRIGRGGR
     FGRKGVAINF VSNEDKNMLE EIETYYNTQV EEMPLNVADL I
//
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