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Database: UniProt
Entry: P0DMI1
LinkDB: P0DMI1
Original site: P0DMI1 
ID   HELS_ARCFU              Reviewed;         691 AA.
AC   P0DMI1;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   14-MAY-2014, sequence version 1.
DT   16-OCT-2019, entry version 27.
DE   RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00442};
GN   Name=hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
GN   OrderedLocusNames=AF_2245;
OS   Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM
OS   9628 / NBRC 100126).
OC   Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales;
OC   Archaeoglobaceae; Archaeoglobus.
OX   NCBI_TaxID=224325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126;
RX   PubMed=9389475; DOI=10.1038/37052;
RA   Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E.,
RA   Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D.,
RA   Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C.,
RA   Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G., Gill S.R.,
RA   Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J.,
RA   Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A.,
RA   Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A.,
RA   Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P.,
RA   Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C.,
RA   Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O.,
RA   Woese C.R., Venter J.C.;
RT   "The complete genome sequence of the hyperthermophilic, sulphate-
RT   reducing archaeon Archaeoglobus fulgidus.";
RL   Nature 390:364-370(1997).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN APO FORM AND IN COMPLEX WITH
RP   DNA, FUNCTION AS A HELICASE, FUNCTION AS AN ATPASE, SUBUNIT, DOMAIN,
RP   AND MUTAGENESIS OF GLU-146.
RX   PubMed=17558417; DOI=10.1038/nsmb1246;
RA   Buttner K., Nehring S., Hopfner K.P.;
RT   "Structural basis for DNA duplex separation by a superfamily-2
RT   helicase.";
RL   Nat. Struct. Mol. Biol. 14:647-652(2007).
CC   -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC       involved in repair of stalled replication forks.
CC       {ECO:0000255|HAMAP-Rule:MF_00442, ECO:0000269|PubMed:17558417}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC   -!- SUBUNIT: Monomer. {ECO:0000305|PubMed:17558417}.
CC   -!- DOMAIN: The N-terminal region (1-400) has DNA-dependent ATPase
CC       activity but no helicase activity. {ECO:0000269|PubMed:17558417}.
CC   -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00442}.
CC   -!- CAUTION: This open reading frame is annotated as an authentic
CC       frameshift in the genome. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AE000782; Type=Frameshift; Evidence={ECO:0000305};
DR   EMBL; AE000782; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PDB; 2P6R; X-ray; 3.00 A; A=1-691.
DR   PDB; 2P6U; X-ray; 3.14 A; A=1-691.
DR   PDBsum; 2P6R; -.
DR   PDBsum; 2P6U; -.
DR   SMR; P0DMI1; -.
DR   eggNOG; arCOG00553; Archaea.
DR   eggNOG; COG1204; LUCA.
DR   Proteomes; UP000002199; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00442; Helicase_Hel308; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR022965; Helicase_Hel308.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Complete proteome; DNA damage; DNA repair;
KW   DNA-binding; Helicase; Hydrolase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN         1    691       ATP-dependent DNA helicase Hel308.
FT                                /FTId=PRO_0000429029.
FT   DOMAIN       34    200       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00442}.
FT   DOMAIN      230    421       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_00442}.
FT   NP_BIND      47     54       ATP. {ECO:0000255|HAMAP-Rule:MF_00442}.
FT   REGION      400    691       Required for helicase but not ATPase
FT                                activity.
FT   MOTIF       145    148       DEAH box.
FT   BINDING      30     30       ATP. {ECO:0000255|HAMAP-Rule:MF_00442}.
FT   MUTAGEN     146    146       E->Q: Loss of DNA-stimulated ATP
FT                                hydrolysis, almost complete loss of DNA
FT                                unwinding. {ECO:0000269|PubMed:17558417}.
SQ   SEQUENCE   691 AA;  77835 MW;  2E5B2D3E69D6A38E CRC64;
     MKVEELAESI SSYAVGILKE EGIEELFPPQ AEAVEKVFSG KNLLLAMPTA AGKTLLAEMA
     MVREAIKGGK SLYVVPLRAL AGEKYESFKK WEKIGLRIGI STGDYESRDE HLGDCDIIVT
     TSEKADSLIR NRASWIKAVS CLVVDEIHLL DSEKRGATLE ILVTKMRRMN KALRVIGLSA
     TAPNVTEIAE WLDADYYVSD WRPVPLVEGV LCEGTLELFD GAFSTSRRVK FEELVEECVA
     ENGGVLVFES TRRGAEKTAV KLSAITAKYV ENEGLEKAIL EENEGEMSRK LAECVRKGAA
     FHHAGLLNGQ RRVVEDAFRR GNIKVVVATP TLAAGVNLPA RRVIVRSLYR FDGYSKRIKV
     SEYKQMAGRA GRPGMDERGE AIIIVGKRDR EIAVKRYIFG EPERITSKLG VETHLRFHSL
     SIICDGYAKT LEELEDFFAD TFFFKQNEIS LSYELERVVR QLENWGMVVE DHHLAPTKLG
     SLVSRLYIDP LTGFIFHDVL SRMELSDIGA LHLICRTPDM ERLTVRKTDS WVEEEAFRLR
     KELSYYPSDF SVEYDWFLSE VKTALCLKDW IEEKDEDEIC AKYGIAPGDL RRIVETAEWL
     SNAMNRIAEE VGNTSVSGLT ERIKHGVKEE LLELVRIRHI GRVRARKLYN AGIRNAEDIV
     RHREKVASLI GRGIAERVVE GISVKSLNPE S
//
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