ID MT_POVBG Reviewed; 421 AA.
AC P0DOJ7; P03076; Q76TX4; Q76W01; Q89765;
DT 20-DEC-2017, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2017, sequence version 1.
DT 08-NOV-2023, entry version 25.
DE RecName: Full=Middle T antigen;
DE Short=MT;
DE Short=MT-AG;
OS Murine polyomavirus (strain BG) (MPyV).
OC Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes;
OC Sepolyvirales; Polyomaviridae; Alphapolyomavirus;
OC Mus musculus polyomavirus 1.
OX NCBI_TaxID=179241;
OH NCBI_TaxID=10090; Mus musculus (Mouse).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Clark B.E., Griffin B.E.;
RL Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Plays a role in transformation by modulating the activities
CC of cellular proteins involved in control of cell proliferation and by
CC acting as a functional homolog of an activated tyrosine kinase-
CC associated growth-factor receptor. Recruits upon association with host
CC Ppp2/PP2A the Src tyrosine kinase components Src, Yes and Fyn, thereby
CC activating their kinase activity. Activation of Shc1, Pclg1 and p85
CC mediate signal transduction pathways leading to cell cycle progression
CC and cell division. MT also plays a role in regulation of early and late
CC gene expression and in viral DNA replication.
CC {ECO:0000250|UniProtKB:P03077}.
CC -!- SUBUNIT: Interacts with host Ppp2/PP2A A and C subunits; this
CC interaction alters Ppp2/PP2A substrate specificity and localization.
CC Interacts with host Src, Yes1, and Fyn. Interacts with host Shc1, Plcg1
CC and p85; these interactions lead to cell cycle progression. Interacts
CC with host 14-3-3 proteins. {ECO:0000250|UniProtKB:P03077}.
CC -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=Middle T antigen;
CC IsoId=P0DOJ7-1, P03076-1;
CC Sequence=Displayed;
CC Name=Small t antigen;
CC IsoId=P0DOJ4-2; Sequence=External;
CC Name=Large T antigen;
CC IsoId=P0DOJ4-1, P03074-1;
CC Sequence=External;
CC -!- DOMAIN: The NPTY motif is required for interaction with host Shc1
CC protein. {ECO:0000250|UniProtKB:P03077}.
CC -!- PTM: Tyrosine-phosphorylated on three residues 250, 315 and 322,
CC providing docking sites for host Shc1, p85, and Plcg1, respectively.
CC {ECO:0000250|UniProtKB:P03077}.
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DR EMBL; AF442959; AAL35608.1; -; Genomic_DNA.
DR RefSeq; YP_009111414.1; NC_001515.2. [P0DOJ7-1]
DR PDB; 1FU5; NMR; -; B=312-326.
DR PDB; 1QJB; X-ray; 2.00 A; Q/S=254-259.
DR PDBsum; 1FU5; -.
DR PDBsum; 1QJB; -.
DR SMR; P0DOJ7; -.
DR GeneID; 22619583; -.
DR KEGG; vg:22619583; -.
DR OrthoDB; 14669at10239; -.
DR Proteomes; UP000116380; Genome.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR Gene3D; 1.10.287.110; DnaJ domain; 1.
DR Gene3D; 1.20.120.1860; Small t-antigen, unique domain; 1.
DR InterPro; IPR001623; DnaJ_domain.
DR InterPro; IPR036869; J_dom_sf.
DR InterPro; IPR003354; Papo_T_antigen.
DR InterPro; IPR036092; Papo_T_antigensf.
DR Pfam; PF02380; Papo_T_antigen; 1.
DR SMART; SM00271; DnaJ; 1.
DR SUPFAM; SSF46565; Chaperone J-domain; 1.
DR SUPFAM; SSF161240; T-antigen specific domain-like; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Early protein; Host membrane;
KW Host-virus interaction; Membrane; Oncogene; Phosphoprotein; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..421
FT /note="Middle T antigen"
FT /id="PRO_0000115048"
FT TOPO_DOM 1..394
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 416..421
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT DOMAIN 12..75
FT /note="J"
FT REGION 215..237
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 250
FT /note="Phosphotyrosine; by host"
FT /evidence="ECO:0000250|UniProtKB:P03077"
FT MOD_RES 257
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000250|UniProtKB:P03077"
FT MOD_RES 315
FT /note="Phosphotyrosine; by host"
FT /evidence="ECO:0000250|UniProtKB:P03077"
FT MOD_RES 322
FT /note="Phosphotyrosine; by host"
FT /evidence="ECO:0000250|UniProtKB:P03077"
SQ SEQUENCE 421 AA; 48694 MW; FC394691F84E8EB5 CRC64;
MDRVLSRADK ERLLELLKLP RQLWGDFGRM QQAYKQQSLL LHPDKGGSHA LMQELNSLWG
TFKTEVYNLR MNLGGTGFQV RRLHADGWNL STKDTFGDRY YQRFCRMPLT CLVNVKYSSC
SCILCLLRKQ HRELKDKCDA RCLVLGECFC LECYMQWFGT PTRDVLNLYA DFIASMPIDW
LDLDVHSVYN PKRRSEELRR AATVHYTMTT GHSAMEASTS QGNGMISSES GTPATSRRLR
LPSLLSNPTY SVMRSHSYPP TRVLQQIHPH ILLEEDEILV LLSPMTAYPR TPPELLYPES
DQDQLEPLEE EEEEYMPMED LYLDILPEEQ VPQLIPPPII PRAGLSPWEG LILRDLQRAH
FDPILDASQR MRATHRAALR AHSMQRHLRR LGRTLLLVTF LAALLGICLM LFILIKRSRH
F
//
