ID PSAF_SPIOL Reviewed; 231 AA.
AC P12355;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 1.
DT 24-JAN-2024, entry version 106.
DE RecName: Full=Photosystem I reaction center subunit III, chloroplastic;
DE AltName: Full=Light-harvesting complex I 17 kDa protein;
DE AltName: Full=PSI-F;
DE Flags: Precursor;
GN Name=PSAF;
OS Spinacia oleracea (Spinach).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3049152; DOI=10.1016/0014-5793(88)80205-9;
RA Steppuhn J., Hermans J., Nechushtai R., Ljungberg U., Thuemmler F.,
RA Lottspeich F., Herrmann R.G.;
RT "Nucleotide sequence of cDNA clones encoding the entire precursor
RT polypeptides for subunits IV and V of the photosystem I reaction center
RT from spinach.";
RL FEBS Lett. 237:218-224(1988).
RN [2]
RP PROTEIN SEQUENCE OF 78-91.
RA Hippler M., Ratajczak R., Haehnel W.;
RT "Identification of the plastocyanin binding subunit of photosystem I.";
RL FEBS Lett. 250:280-284(1989).
CC -!- FUNCTION: Probably participates in efficiency of electron transfer from
CC plastocyanin to P700 (or cytochrome c553 in algae and cyanobacteria).
CC This plastocyanin-docking protein contributes to the specific
CC association of plastocyanin to PSI.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen.
CC -!- SIMILARITY: Belongs to the PsaF family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X13133; CAA31523.1; -; mRNA.
DR PIR; S00451; F1SP3.
DR PDB; 2O01; X-ray; 3.40 A; F=78-231.
DR PDB; 2WSC; X-ray; 3.30 A; F=1-231.
DR PDB; 2WSE; X-ray; 3.49 A; F=1-231.
DR PDB; 2WSF; X-ray; 3.48 A; F=1-231.
DR PDB; 3LW5; X-ray; 3.30 A; F=78-231.
DR PDB; 4XK8; X-ray; 2.80 A; F/f=78-228.
DR PDB; 6LY5; EM; 2.38 A; f=78-228.
DR PDBsum; 2O01; -.
DR PDBsum; 2WSC; -.
DR PDBsum; 2WSE; -.
DR PDBsum; 2WSF; -.
DR PDBsum; 3LW5; -.
DR PDBsum; 4XK8; -.
DR PDBsum; 6LY5; -.
DR AlphaFoldDB; P12355; -.
DR SMR; P12355; -.
DR DIP; DIP-61659N; -.
DR IntAct; P12355; 2.
DR MINT; P12355; -.
DR OrthoDB; 1217125at2759; -.
DR EvolutionaryTrace; P12355; -.
DR Proteomes; UP001155700; Unplaced.
DR GO; GO:0009543; C:chloroplast thylakoid lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0009538; C:photosystem I reaction center; IEA:InterPro.
DR GO; GO:0051219; F:phosphoprotein binding; IPI:CAFA.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR DisProt; DP00990; -.
DR Gene3D; 1.10.8.110; Photosystem I PsaF, reaction centre subunit III; 1.
DR InterPro; IPR003666; PSI_PsaF.
DR InterPro; IPR036577; PSI_PsaF_sf.
DR PANTHER; PTHR34939; PHOTOSYSTEM I REACTION CENTER SUBUNIT III, CHLOROPLASTIC; 1.
DR PANTHER; PTHR34939:SF1; PHOTOSYSTEM I REACTION CENTER SUBUNIT III, CHLOROPLASTIC; 1.
DR Pfam; PF02507; PSI_PsaF; 1.
DR SUPFAM; SSF81536; Subunit III of photosystem I reaction centre, PsaF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Photosynthesis;
KW Photosystem I; Plastid; Reference proteome; Thylakoid; Transit peptide.
FT TRANSIT 1..77
FT /note="Chloroplast"
FT /evidence="ECO:0000269|Ref.2"
FT CHAIN 78..231
FT /note="Photosystem I reaction center subunit III,
FT chloroplastic"
FT /id="PRO_0000029346"
FT HELIX 79..81
FT /evidence="ECO:0007829|PDB:6LY5"
FT HELIX 85..87
FT /evidence="ECO:0007829|PDB:6LY5"
FT HELIX 89..106
FT /evidence="ECO:0007829|PDB:6LY5"
FT HELIX 114..136
FT /evidence="ECO:0007829|PDB:6LY5"
FT STRAND 142..144
FT /evidence="ECO:0007829|PDB:3LW5"
FT STRAND 146..148
FT /evidence="ECO:0007829|PDB:6LY5"
FT STRAND 151..155
FT /evidence="ECO:0007829|PDB:2WSC"
FT HELIX 157..159
FT /evidence="ECO:0007829|PDB:6LY5"
FT HELIX 161..184
FT /evidence="ECO:0007829|PDB:6LY5"
FT STRAND 187..189
FT /evidence="ECO:0007829|PDB:6LY5"
FT HELIX 193..196
FT /evidence="ECO:0007829|PDB:6LY5"
FT HELIX 200..207
FT /evidence="ECO:0007829|PDB:6LY5"
FT HELIX 208..212
FT /evidence="ECO:0007829|PDB:6LY5"
FT HELIX 213..221
FT /evidence="ECO:0007829|PDB:6LY5"
FT STRAND 224..227
FT /evidence="ECO:0007829|PDB:6LY5"
FT HELIX 228..230
FT /evidence="ECO:0007829|PDB:2WSC"
SQ SEQUENCE 231 AA; 25412 MW; 270E1BAC159D79B4 CRC64;
MSFTIPTNLY KPLATKPKHL SSSSFAPRSK IVCQQENDQQ QPKKLELAKV GANAAAALAL
SSVLLSSWSV APDAAMADIA GLTPCKESKQ FAKREKQALK KLQASLKLYA DDSAPALAIK
ATMEKTKKRF DNYGKYGLLC GSDGLPHLIV SGDQRHWGEF ITPGILFLYI AGWIGWVGRS
YLIAIRDEKK PTQKEIIIDV PLASSLLFRG FSWPVAAYRE LLNGELVDNN F
//
