ID FECE_ECOLI Reviewed; 255 AA.
AC P15031; Q2M628;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 27-MAR-2024, entry version 168.
DE RecName: Full=Fe(3+) dicitrate transport ATP-binding protein FecE {ECO:0000305};
DE EC=7.2.2.18 {ECO:0000305|PubMed:1526456, ECO:0000305|PubMed:2651410};
DE AltName: Full=Iron(III) dicitrate transport ATP-binding protein FecE;
GN Name=fecE {ECO:0000303|PubMed:2651410}; OrderedLocusNames=b4287, JW4247;
OS Escherichia coli (strain K12).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=K12;
RX PubMed=2651410; DOI=10.1128/jb.171.5.2626-2633.1989;
RA Staudenmaier H., van Hove B., Yaraghi Z., Braun V.;
RT "Nucleotide sequences of the fecBCDE genes and locations of the proteins
RT suggest a periplasmic-binding-protein-dependent transport mechanism for
RT iron(III) dicitrate in Escherichia coli.";
RL J. Bacteriol. 171:2626-2633(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION, AND ATP-BINDING.
RX PubMed=1526456; DOI=10.1016/0378-1097(92)90434-p;
RA Schultz-Hauser G., Van Hove B., Braun V.;
RT "8-Azido-ATP labelling of the FecE protein of the Escherichia coli iron
RT citrate transport system.";
RL FEMS Microbiol. Lett. 74:231-234(1992).
RN [6]
RP INDUCTION BY HYDROXYUREA.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=20005847; DOI=10.1016/j.molcel.2009.11.024;
RA Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J.,
RA Walker G.C.;
RT "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia
RT coli.";
RL Mol. Cell 36:845-860(2009).
CC -!- FUNCTION: Part of the ABC transporter complex FecBCDE involved in
CC citrate-dependent Fe(3+) uptake (PubMed:2651410). Binds ATP
CC (PubMed:1526456). Probably responsible for energy coupling to the
CC transport system (PubMed:1526456). {ECO:0000269|PubMed:1526456,
CC ECO:0000269|PubMed:2651410}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + iron(III) dicitrate(out) = ADP + H(+) + iron(III)
CC dicitrate(in) + phosphate; Xref=Rhea:RHEA:58912, ChEBI:CHEBI:4991,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.2.2.18;
CC Evidence={ECO:0000305|PubMed:1526456, ECO:0000305|PubMed:2651410};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (FecE),
CC two transmembrane proteins (FecC and FecD) and a solute-binding protein
CC (FecB). {ECO:0000269|PubMed:2651410}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:2651410};
CC Peripheral membrane protein {ECO:0000305|PubMed:2651410}.
CC -!- INDUCTION: Induced 1.6-fold by hydroxyurea.
CC {ECO:0000269|PubMed:20005847}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
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DR EMBL; M26397; AAA23765.1; -; Genomic_DNA.
DR EMBL; U14003; AAA97183.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77243.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78278.1; -; Genomic_DNA.
DR PIR; JS0115; QRECM3.
DR RefSeq; NP_418707.1; NC_000913.3.
DR RefSeq; WP_000175457.1; NZ_STEB01000013.1.
DR AlphaFoldDB; P15031; -.
DR SMR; P15031; -.
DR BioGRID; 4262739; 249.
DR ComplexPortal; CPX-4403; Ferric-citrate ABC transporter complex.
DR IntAct; P15031; 2.
DR STRING; 511145.b4287; -.
DR TCDB; 3.A.1.14.1; the atp-binding cassette (abc) superfamily.
DR PaxDb; 511145-b4287; -.
DR EnsemblBacteria; AAC77243; AAC77243; b4287.
DR GeneID; 948819; -.
DR KEGG; ecj:JW4247; -.
DR KEGG; eco:b4287; -.
DR PATRIC; fig|1411691.4.peg.2413; -.
DR EchoBASE; EB0286; -.
DR eggNOG; COG1120; Bacteria.
DR HOGENOM; CLU_000604_1_11_6; -.
DR InParanoid; P15031; -.
DR OMA; DKIWLMD; -.
DR OrthoDB; 5292475at2; -.
DR PhylomeDB; P15031; -.
DR BioCyc; EcoCyc:FECE-MONOMER; -.
DR BioCyc; MetaCyc:FECE-MONOMER; -.
DR PHI-base; PHI:11754; -.
DR PHI-base; PHI:8010; -.
DR PRO; PR:P15031; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; NAS:ComplexPortal.
DR GO; GO:0016020; C:membrane; NAS:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0005524; F:ATP binding; IDA:EcoCyc.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0006879; P:intracellular iron ion homeostasis; NAS:ComplexPortal.
DR GO; GO:0033212; P:iron import into cell; NAS:ComplexPortal.
DR GO; GO:0055085; P:transmembrane transport; IEA:GOC.
DR CDD; cd03214; ABC_Iron-Siderophores_B12_Hemin; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR42771:SF12; FE(3+) DICITRATE TRANSPORT ATP-BINDING PROTEIN FECE-RELATED; 1.
DR PANTHER; PTHR42771; IRON(3+)-HYDROXAMATE IMPORT ATP-BINDING PROTEIN FHUC; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell inner membrane; Cell membrane; Ion transport; Iron;
KW Iron transport; Membrane; Nucleotide-binding; Reference proteome;
KW Translocase; Transport.
FT CHAIN 1..255
FT /note="Fe(3+) dicitrate transport ATP-binding protein FecE"
FT /id="PRO_0000092324"
FT DOMAIN 3..238
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 35..42
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ SEQUENCE 255 AA; 28191 MW; 89785C2A91D51FF3 CRC64;
MTLRTENLTV SYGTDKVLND VSLSLPTGKI TALIGPNGCG KSTLLNCFSR LLMPQSGTVF
LGDNPINMLS SRQLARRLSL LPQHHLTPEG ITVQELVSYG RNPWLSLWGR LSAEDNARVN
VAMNQTRINH LAVRRLTELS GGQRQRAFLA MVLAQNTPVV LLDEPTTYLD INHQVDLMRL
MGELRTQGKT VVAVLHDLNQ ASRYCDQLVV MANGHVMAQG TPEEVMTPGL LRTVFSVEAE
IHPEPVSGRP MCLMR
//
