GenomeNet

Database: UniProt
Entry: P15038
LinkDB: P15038
Original site: P15038 
ID   HELD_ECOLI              Reviewed;         684 AA.
AC   P15038; P77623;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   28-MAR-2018, entry version 153.
DE   RecName: Full=DNA helicase IV;
DE            EC=3.6.4.12;
DE   AltName: Full=75 kDa helicase;
GN   Name=helD; OrderedLocusNames=b0962, JW0945;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-27.
RX   PubMed=2542273;
RA   Wood E.R., Matson S.W.;
RT   "The molecular cloning of the gene encoding the Escherichia coli 75-
RT   kDa helicase and the determination of its nucleotide sequence and
RT   gentic map position.";
RL   J. Biol. Chem. 264:8297-8303(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A.,
RA   Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K.,
RA   Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K.,
RA   Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N.,
RA   Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y.,
RA   Yano M., Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome
RT   corresponding to the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Helicase IV catalyzes the unwinding of duplex DNA in the
CC       3' to 5' direction with respect to the bound single strand in a
CC       reaction that is dependent upon the hydrolysis of ATP.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC   -!- INTERACTION:
CC       P00936:cyaA; NbExp=3; IntAct=EBI-551473, EBI-1116685;
CC       P0CE47:tufA; NbExp=3; IntAct=EBI-551473, EBI-301077;
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000305}.
DR   EMBL; J04726; AAA23952.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74048.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35727.1; -; Genomic_DNA.
DR   PIR; A64837; HJECD4.
DR   RefSeq; NP_415482.1; NC_000913.3.
DR   RefSeq; WP_001295354.1; NZ_LN832404.1.
DR   ProteinModelPortal; P15038; -.
DR   SMR; P15038; -.
DR   BioGrid; 4263362; 127.
DR   DIP; DIP-9876N; -.
DR   IntAct; P15038; 35.
DR   STRING; 316385.ECDH10B_1032; -.
DR   BindingDB; P15038; -.
DR   ChEMBL; CHEMBL4405; -.
DR   PaxDb; P15038; -.
DR   PRIDE; P15038; -.
DR   EnsemblBacteria; AAC74048; AAC74048; b0962.
DR   EnsemblBacteria; BAA35727; BAA35727; BAA35727.
DR   GeneID; 946227; -.
DR   KEGG; ecj:JW0945; -.
DR   KEGG; eco:b0962; -.
DR   PATRIC; fig|1411691.4.peg.1312; -.
DR   EchoBASE; EB0421; -.
DR   EcoGene; EG10426; helD.
DR   eggNOG; ENOG41088T4; Bacteria.
DR   eggNOG; COG0210; LUCA.
DR   HOGENOM; HOG000118816; -.
DR   InParanoid; P15038; -.
DR   KO; K03658; -.
DR   OMA; DVSPQIV; -.
DR   PhylomeDB; P15038; -.
DR   BioCyc; EcoCyc:EG10426-MONOMER; -.
DR   PRO; PR:P15038; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0033202; C:DNA helicase complex; IDA:EcoCyc.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043140; F:ATP-dependent 3'-5' DNA helicase activity; IDA:EcoCyc.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IMP:EcoCyc.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR022161; Helicase_IV_N.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   InterPro; IPR034739; UvrD/AddA_N.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   Pfam; PF12462; Helicase_IV_N; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Complete proteome; Direct protein sequencing;
KW   DNA-binding; Helicase; Hydrolase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN         1    684       DNA helicase IV.
FT                                /FTId=PRO_0000102049.
FT   DOMAIN      195    505       UvrD-like helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00560}.
FT   NP_BIND     216    223       ATP. {ECO:0000305}.
FT   BINDING     503    503       ATP. {ECO:0000250}.
FT   CONFLICT     70     70       H -> P (in Ref. 1; AAA23952).
FT                                {ECO:0000305}.
FT   CONFLICT    137    140       SALP -> LRVA (in Ref. 1; AAA23952).
FT                                {ECO:0000305}.
FT   CONFLICT    365    366       MH -> ID (in Ref. 1; AAA23952).
FT                                {ECO:0000305}.
SQ   SEQUENCE   684 AA;  77976 MW;  FC1A6250537A4A24 CRC64;
     MELKATTLGK RLAQHPYDRA VILNAGIKVS GDRHEYLIPF NQLLAIHCKR GLVWGELEFV
     LPDEKVVRLH GTEWGETQRF YHHLDAHWRR WSGEMSEIAS GVLRQQLDLI ATRTGENKWL
     TREQTSGVQQ QIRQALSALP LPVNRLEEFD NCREAWRKCQ AWLKDIESAR LQHNQAYTEA
     MLTEYADFFR QVESSPLNPA QARAVVNGEH SLLVLAGAGS GKTSVLVARA GWLLARGEAS
     PEQILLLAFG RKAAEEMDER IRERLHTEDI TARTFHALAL HIIQQGSKKV PIVSKLENDT
     AARHELFIAE WRKQCSEKKA QAKGWRQWLT EEMQWSVPEG NFWDDEKLQR RLASRLDRWV
     SLMRMHGGAQ AEMIASAPEE IRDLFSKRIK LMAPLLKAWK GALKAENAVD FSGLIHQAIV
     ILEKGRFISP WKHILVDEFQ DISPQRAALL AALRKQNSQT TLFAVGDDWQ AIYRFSGAQM
     SLTTAFHENF GEGERCDLDT TYRFNSRIGE VANRFIQQNP GQLKKPLNSL TNGDKKAVTL
     LDESQLDALL DKLSGYAKPE ERILILARYH HMRPASLEKA ATRWPKLQID FMTIHASKGQ
     QADYVIIVGL QEGSDGFPAA ARESIMEEAL LPPVEDFPDA EERRLMYVAL TRARHRVWAL
     FNKENPSPFV EILKNLDVPV ARKP
//
DBGET integrated database retrieval system