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Database: UniProt
Entry: P17412
LinkDB: P17412
Original site: P17412 
ID   FRDA_WOLSU              Reviewed;         656 AA.
AC   P17412;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2003, sequence version 3.
DT   08-MAY-2019, entry version 150.
DE   RecName: Full=Fumarate reductase flavoprotein subunit;
DE            EC=1.3.5.4;
GN   Name=frdA; OrderedLocusNames=WS0831;
OS   Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC
OS   11488 / FDC 602W) (Vibrio succinogenes).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Wolinella.
OX   NCBI_TaxID=273121;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2244791; DOI=10.1007/BF00276536;
RA   Lauterbach F., Koertner C., Albracht S.P., Unden G., Kroeger A.;
RT   "The fumarate reductase operon of Wolinella succinogenes. Sequence and
RT   expression of the frdA and frdB genes.";
RL   Arch. Microbiol. 154:386-393(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=9492313; DOI=10.1046/j.1432-1327.1998.2510418.x;
RA   Simon J., Gross R., Ringel M., Schmidt E., Kroeger A.;
RT   "Deletion and site-directed mutagenesis of the Wolinella succinogenes
RT   fumarate reductase operon.";
RL   Eur. J. Biochem. 251:418-426(1998).
RN   [3]
RP   SEQUENCE REVISION TO 281-289; 490 AND 593.
RA   Simon J.;
RL   Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W;
RX   PubMed=14500908; DOI=10.1073/pnas.1932838100;
RA   Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O.,
RA   Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B.,
RA   Meyer F., Lederer H., Schuster S.C.;
RT   "Complete genome sequence and analysis of Wolinella succinogenes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.
RX   PubMed=3609021; DOI=10.1111/j.1432-1033.1987.tb13536.x;
RA   Lauterbach F., Kortner C., Tripier D., Unden G.;
RT   "Cloning and expression of the genes of two fumarate reductase
RT   subunits from Wolinella succinogenes.";
RL   Eur. J. Biochem. 166:447-452(1987).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS), AND SEQUENCE REVISION TO
RP   281-289.
RX   PubMed=10586875; DOI=10.1038/46483;
RA   Lancaster C.R.D., Kroeger A., Auer M., Michel H.;
RT   "Structure of fumarate reductase from Wolinella succinogenes at 2.2 A
RT   resolution.";
RL   Nature 402:377-385(1999).
RN   [7]
RP   X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
RX   PubMed=11248702; DOI=10.1046/j.1432-1033.2001.02053.x;
RA   Lancaster C.R.D., Gross R., Simon J.;
RT   "A third crystal form of Wolinella succinogenes quinol:fumarate
RT   reductase reveals domain closure at the site of fumarate reduction.";
RL   Eur. J. Biochem. 268:1820-1827(2001).
CC   -!- FUNCTION: The fumarate reductase enzyme complex is required for
CC       fumarate respiration using formate or sulfide as electron donor.
CC       {ECO:0000269|PubMed:9492313}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a quinone + succinate = a quinol + fumarate;
CC         Xref=Rhea:RHEA:40523, ChEBI:CHEBI:24646, ChEBI:CHEBI:29806,
CC         ChEBI:CHEBI:30031, ChEBI:CHEBI:132124; EC=1.3.5.4;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC       Note=Binds 1 FAD covalently per subunit.;
CC   -!- SUBUNIT: Part of an enzyme complex containing three subunits: a
CC       flavoprotein, an iron-sulfur protein, and cytochrome b-556.
CC   -!- SIMILARITY: Belongs to the FAD-dependent oxidoreductase 2 family.
CC       FRD/SDH subfamily. {ECO:0000305}.
DR   EMBL; AJ000662; CAA04214.2; -; Genomic_DNA.
DR   EMBL; BX571659; CAE09942.1; -; Genomic_DNA.
DR   EMBL; M28294; AAA27586.1; -; Genomic_DNA.
DR   PIR; B44954; B44954.
DR   RefSeq; WP_011138739.1; NC_005090.1.
DR   PDB; 1E7P; X-ray; 3.10 A; A/D/G/J=1-656.
DR   PDB; 1QLB; X-ray; 2.33 A; A/D=1-656.
DR   PDB; 2BS2; X-ray; 1.78 A; A/D=1-656.
DR   PDB; 2BS3; X-ray; 2.19 A; A/D=1-656.
DR   PDB; 2BS4; X-ray; 2.76 A; A/D=1-656.
DR   PDBsum; 1E7P; -.
DR   PDBsum; 1QLB; -.
DR   PDBsum; 2BS2; -.
DR   PDBsum; 2BS3; -.
DR   PDBsum; 2BS4; -.
DR   SMR; P17412; -.
DR   STRING; 273121.WS0831; -.
DR   TCDB; 3.D.10.1.3; the prokaryotic succinate dehydrogenase (sdh) family.
DR   EnsemblBacteria; CAE09942; CAE09942; WS0831.
DR   KEGG; wsu:WS0831; -.
DR   eggNOG; ENOG4105C00; Bacteria.
DR   eggNOG; COG1053; LUCA.
DR   HOGENOM; HOG000160475; -.
DR   KO; K00244; -.
DR   OMA; HRAESCG; -.
DR   OrthoDB; 153138at2; -.
DR   BioCyc; WSUC273121:G1GTD-799-MONOMER; -.
DR   EvolutionaryTrace; P17412; -.
DR   Proteomes; UP000000422; Chromosome.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0102040; F:fumarate reductase (menaquinone); IEA:UniProtKB-EC.
DR   GO; GO:0022900; P:electron transport chain; IEA:InterPro.
DR   Gene3D; 3.50.50.60; -; 1.
DR   Gene3D; 3.90.700.10; -; 1.
DR   InterPro; IPR003953; FAD-binding_2.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR003952; FRD_SDH_FAD_BS.
DR   InterPro; IPR037099; Fum_R/Succ_DH_flav-like_C_sf.
DR   InterPro; IPR015939; Fum_Rdtase/Succ_DH_flav-like_C.
DR   InterPro; IPR027477; Succ_DH/fumarate_Rdtase_cat_sf.
DR   InterPro; IPR014006; Succ_Dhase_FrdA_Gneg.
DR   Pfam; PF00890; FAD_binding_2; 1.
DR   Pfam; PF02910; Succ_DH_flav_C; 1.
DR   SUPFAM; SSF46977; SSF46977; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   SUPFAM; SSF56425; SSF56425; 1.
DR   TIGRFAMs; TIGR01812; sdhA_frdA_Gneg; 1.
DR   PROSITE; PS00504; FRD_SDH_FAD_BINDING; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Electron transport; FAD;
KW   Flavoprotein; Oxidoreductase; Reference proteome; Respiratory chain;
KW   Transport.
FT   CHAIN         1    656       Fumarate reductase flavoprotein subunit.
FT                                /FTId=PRO_0000158667.
FT   NP_BIND       9     23       FAD. {ECO:0000255}.
FT   ACT_SITE    257    257       {ECO:0000250}.
FT   ACT_SITE    273    273       {ECO:0000250}.
FT   MOD_RES      43     43       Tele-8alpha-FAD histidine.
FT   STRAND        3      5       {ECO:0000244|PDB:2BS2}.
FT   STRAND        7     11       {ECO:0000244|PDB:2BS2}.
FT   HELIX        15     25       {ECO:0000244|PDB:2BS2}.
FT   TURN         26     28       {ECO:0000244|PDB:2BS2}.
FT   STRAND       31     34       {ECO:0000244|PDB:2BS2}.
FT   HELIX        39     41       {ECO:0000244|PDB:2BS2}.
FT   HELIX        43     46       {ECO:0000244|PDB:2BS2}.
FT   HELIX        59     61       {ECO:0000244|PDB:2BS2}.
FT   HELIX        66     76       {ECO:0000244|PDB:2BS2}.
FT   TURN         77     79       {ECO:0000244|PDB:2BS2}.
FT   HELIX        83    102       {ECO:0000244|PDB:2BS2}.
FT   STRAND      112    116       {ECO:0000244|PDB:2BS2}.
FT   STRAND      119    122       {ECO:0000244|PDB:1QLB}.
FT   STRAND      125    128       {ECO:0000244|PDB:2BS2}.
FT   HELIX       130    132       {ECO:0000244|PDB:2BS2}.
FT   HELIX       156    171       {ECO:0000244|PDB:2BS2}.
FT   STRAND      174    176       {ECO:0000244|PDB:2BS2}.
FT   STRAND      178    187       {ECO:0000244|PDB:2BS2}.
FT   STRAND      190    198       {ECO:0000244|PDB:2BS2}.
FT   TURN        199    201       {ECO:0000244|PDB:2BS2}.
FT   STRAND      204    208       {ECO:0000244|PDB:2BS2}.
FT   STRAND      210    214       {ECO:0000244|PDB:2BS2}.
FT   HELIX       220    222       {ECO:0000244|PDB:2BS2}.
FT   STRAND      223    228       {ECO:0000244|PDB:2BS2}.
FT   HELIX       235    241       {ECO:0000244|PDB:2BS2}.
FT   STRAND      244    246       {ECO:0000244|PDB:2BS2}.
FT   STRAND      254    258       {ECO:0000244|PDB:2BS2}.
FT   TURN        262    264       {ECO:0000244|PDB:2BS2}.
FT   HELIX       271    275       {ECO:0000244|PDB:2BS2}.
FT   STRAND      278    280       {ECO:0000244|PDB:2BS2}.
FT   STRAND      282    285       {ECO:0000244|PDB:1E7P}.
FT   HELIX       288    291       {ECO:0000244|PDB:2BS2}.
FT   TURN        293    295       {ECO:0000244|PDB:2BS2}.
FT   HELIX       296    298       {ECO:0000244|PDB:2BS2}.
FT   HELIX       301    313       {ECO:0000244|PDB:2BS2}.
FT   TURN        314    316       {ECO:0000244|PDB:2BS2}.
FT   STRAND      317    320       {ECO:0000244|PDB:2BS4}.
FT   STRAND      325    329       {ECO:0000244|PDB:2BS2}.
FT   HELIX       331    333       {ECO:0000244|PDB:2BS2}.
FT   HELIX       335    341       {ECO:0000244|PDB:2BS2}.
FT   HELIX       343    351       {ECO:0000244|PDB:2BS2}.
FT   TURN        357    359       {ECO:0000244|PDB:2BS2}.
FT   STRAND      362    364       {ECO:0000244|PDB:2BS2}.
FT   STRAND      367    371       {ECO:0000244|PDB:2BS2}.
FT   STRAND      374    376       {ECO:0000244|PDB:2BS2}.
FT   STRAND      384    386       {ECO:0000244|PDB:2BS2}.
FT   STRAND      388    390       {ECO:0000244|PDB:2BS2}.
FT   HELIX       392    394       {ECO:0000244|PDB:2BS2}.
FT   HELIX       408    430       {ECO:0000244|PDB:2BS2}.
FT   STRAND      434    436       {ECO:0000244|PDB:2BS2}.
FT   HELIX       437    456       {ECO:0000244|PDB:2BS2}.
FT   HELIX       463    477       {ECO:0000244|PDB:2BS2}.
FT   STRAND      478    482       {ECO:0000244|PDB:2BS2}.
FT   HELIX       484    501       {ECO:0000244|PDB:2BS2}.
FT   STRAND      511    513       {ECO:0000244|PDB:2BS2}.
FT   HELIX       515    539       {ECO:0000244|PDB:2BS2}.
FT   STRAND      547    549       {ECO:0000244|PDB:1E7P}.
FT   STRAND      554    556       {ECO:0000244|PDB:2BS2}.
FT   TURN        557    559       {ECO:0000244|PDB:2BS2}.
FT   STRAND      562    567       {ECO:0000244|PDB:2BS2}.
FT   STRAND      570    572       {ECO:0000244|PDB:1E7P}.
FT   STRAND      577    582       {ECO:0000244|PDB:2BS2}.
FT   HELIX       585    587       {ECO:0000244|PDB:2BS2}.
FT   STRAND      596    598       {ECO:0000244|PDB:2BS2}.
FT   HELIX       607    625       {ECO:0000244|PDB:2BS2}.
FT   HELIX       630    637       {ECO:0000244|PDB:2BS2}.
FT   HELIX       644    646       {ECO:0000244|PDB:2BS2}.
SQ   SEQUENCE   656 AA;  72719 MW;  BFEE6E8316F7F618 CRC64;
     MKVQYCDSLV IGGGLAGLRA AVATQQKGLS TIVLSLIPVK RSHSAAAQGG MQASLGNSKM
     SDGDNEDLHF MDTVKGSDWG CDQKVARMFV NTAPKAIREL AAWGVPWTRI HKGDRMAIIN
     AQKTTITEED FRHGLIHSRD FGGTKKWRTC YTADATGHTM LFAVANECLK LGVSIQDRKE
     AIALIHQDGK CYGAVVRDLV TGDIIAYVAK GTLIATGGYG RIYKNTTNAV VCEGTGTAIA
     LETGIAQLGN MEAVQFHPTP LFPSGILLTE GCRGDGGILR DVDGHRFMPD YEPEKKELAS
     RDVVSRRMIE HIRKGKGVQS PYGQHLWLDI SILGRKHIET NLRDVQEICE YFAGIDPAEK
     WAPVLPMQHY SMGGIRTDYR GEAKLKGLFS AGEAACWDMH GFNRLGGNSV SEAVVAGMIV
     GEYFAEHCAN TQVDLETKTL EKFVKGQEAY MKSLVESKGT EDVFKIKNRM KDVMDDNVGI
     FRDGPHLEKA VKELEELYKK SKNVGIKNKR LHANPELEEA YRVPMMLKVA LCVAKGALDR
     TESRGAHNRE DYPKRDDINW LNRTLASWPN PEQTLPTLEY EALDVNEMEI APGYRGYGAK
     GNYIENPLSV KRQEEIDKIQ SELEAAGKDR HAIQEALMPY ELPAKYKARN ERLGDK
//
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