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Database: UniProt
Entry: P17596
LinkDB: P17596
Original site: P17596 
ID   FRDB_WOLSU              Reviewed;         239 AA.
AC   P17596;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   08-MAY-2019, entry version 151.
DE   RecName: Full=Fumarate reductase iron-sulfur subunit;
DE            EC=1.3.5.1;
GN   Name=frdB; OrderedLocusNames=WS0830;
OS   Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC
OS   11488 / FDC 602W) (Vibrio succinogenes).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Wolinella.
OX   NCBI_TaxID=273121;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2244791; DOI=10.1007/BF00276536;
RA   Lauterbach F., Koertner C., Albracht S.P., Unden G., Kroeger A.;
RT   "The fumarate reductase operon of Wolinella succinogenes. Sequence and
RT   expression of the frdA and frdB genes.";
RL   Arch. Microbiol. 154:386-393(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=9492313; DOI=10.1046/j.1432-1327.1998.2510418.x;
RA   Simon J., Gross R., Ringel M., Schmidt E., Kroeger A.;
RT   "Deletion and site-directed mutagenesis of the Wolinella succinogenes
RT   fumarate reductase operon.";
RL   Eur. J. Biochem. 251:418-426(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W;
RX   PubMed=14500908; DOI=10.1073/pnas.1932838100;
RA   Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O.,
RA   Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B.,
RA   Meyer F., Lederer H., Schuster S.C.;
RT   "Complete genome sequence and analysis of Wolinella succinogenes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
RX   PubMed=10586875; DOI=10.1038/46483;
RA   Lancaster C.R.D., Kroeger A., Auer M., Michel H.;
RT   "Structure of fumarate reductase from Wolinella succinogenes at 2.2 A
RT   resolution.";
RL   Nature 402:377-385(1999).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
RX   PubMed=11248702; DOI=10.1046/j.1432-1033.2001.02053.x;
RA   Lancaster C.R.D., Gross R., Simon J.;
RT   "A third crystal form of Wolinella succinogenes quinol:fumarate
RT   reductase reveals domain closure at the site of fumarate reduction.";
RL   Eur. J. Biochem. 268:1820-1827(2001).
CC   -!- FUNCTION: The fumarate reductase enzyme complex is required for
CC       fumarate respiration using formate or sulfide as electron donor.
CC       {ECO:0000269|PubMed:9492313}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a quinone + succinate = a quinol + fumarate;
CC         Xref=Rhea:RHEA:40523, ChEBI:CHEBI:24646, ChEBI:CHEBI:29806,
CC         ChEBI:CHEBI:30031, ChEBI:CHEBI:132124; EC=1.3.5.1;
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:49601;
CC       Note=Binds 1 [2Fe-2S] cluster.;
CC   -!- COFACTOR:
CC       Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137;
CC       Note=Binds 1 [3Fe-4S] cluster.;
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC       Note=Binds 1 [4Fe-4S] cluster.;
CC   -!- SUBUNIT: Part of an enzyme complex containing three subunits: a
CC       flavoprotein, an iron-sulfur protein, and cytochrome b-556.
CC   -!- SIMILARITY: Belongs to the succinate dehydrogenase/fumarate
CC       reductase iron-sulfur protein family. {ECO:0000305}.
DR   EMBL; AJ000662; CAA04215.1; -; Genomic_DNA.
DR   EMBL; BX571659; CAE09941.1; -; Genomic_DNA.
DR   PIR; C44954; C44954.
DR   RefSeq; WP_011138738.1; NC_005090.1.
DR   PDB; 1E7P; X-ray; 3.10 A; B/E/H/K=1-239.
DR   PDB; 1QLB; X-ray; 2.33 A; B/E=1-239.
DR   PDB; 2BS2; X-ray; 1.78 A; B/E=1-239.
DR   PDB; 2BS3; X-ray; 2.19 A; B/E=1-239.
DR   PDB; 2BS4; X-ray; 2.76 A; B/E=1-239.
DR   PDBsum; 1E7P; -.
DR   PDBsum; 1QLB; -.
DR   PDBsum; 2BS2; -.
DR   PDBsum; 2BS3; -.
DR   PDBsum; 2BS4; -.
DR   SMR; P17596; -.
DR   STRING; 273121.WS0830; -.
DR   TCDB; 3.D.10.1.3; the prokaryotic succinate dehydrogenase (sdh) family.
DR   EnsemblBacteria; CAE09941; CAE09941; WS0830.
DR   KEGG; wsu:WS0830; -.
DR   eggNOG; ENOG4105E33; Bacteria.
DR   eggNOG; COG0479; LUCA.
DR   HOGENOM; HOG000160590; -.
DR   KO; K00245; -.
DR   OMA; CTHCYRC; -.
DR   OrthoDB; 1605514at2; -.
DR   BioCyc; WSUC273121:G1GTD-798-MONOMER; -.
DR   EvolutionaryTrace; P17596; -.
DR   Proteomes; UP000000422; Chromosome.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008177; F:succinate dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1060.10; -; 1.
DR   Gene3D; 3.10.20.30; -; 1.
DR   InterPro; IPR036010; 2Fe-2S_ferredoxin-like_sf.
DR   InterPro; IPR001041; 2Fe-2S_ferredoxin-type.
DR   InterPro; IPR006058; 2Fe2S_fd_BS.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR   InterPro; IPR012675; Beta-grasp_dom_sf.
DR   InterPro; IPR009051; Helical_ferredxn.
DR   InterPro; IPR004489; Succ_DH/fum_Rdtase_Fe-S.
DR   InterPro; IPR025192; Succ_DH/fum_Rdtase_N.
DR   Pfam; PF13085; Fer2_3; 1.
DR   Pfam; PF13183; Fer4_8; 1.
DR   SUPFAM; SSF46548; SSF46548; 1.
DR   SUPFAM; SSF54292; SSF54292; 1.
DR   TIGRFAMs; TIGR00384; dhsB; 1.
DR   PROSITE; PS00197; 2FE2S_FER_1; 1.
DR   PROSITE; PS51085; 2FE2S_FER_2; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 1.
DR   PROSITE; PS51379; 4FE4S_FER_2; 1.
PE   1: Evidence at protein level;
KW   2Fe-2S; 3D-structure; 3Fe-4S; 4Fe-4S; Complete proteome;
KW   Electron transport; Iron; Iron-sulfur; Metal-binding; Oxidoreductase;
KW   Reference proteome; Respiratory chain; Transport;
KW   Tricarboxylic acid cycle.
FT   CHAIN         1    239       Fumarate reductase iron-sulfur subunit.
FT                                /FTId=PRO_0000158708.
FT   DOMAIN        5     95       2Fe-2S ferredoxin-type.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00465}.
FT   DOMAIN      142    171       4Fe-4S ferredoxin-type.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT   METAL        57     57       Iron-sulfur 1 (2Fe-2S).
FT   METAL        62     62       Iron-sulfur 1 (2Fe-2S).
FT   METAL        65     65       Iron-sulfur 1 (2Fe-2S).
FT   METAL        77     77       Iron-sulfur 1 (2Fe-2S).
FT   METAL       151    151       Iron-sulfur 2 (4Fe-4S).
FT   METAL       154    154       Iron-sulfur 2 (4Fe-4S).
FT   METAL       157    157       Iron-sulfur 2 (4Fe-4S).
FT   METAL       161    161       Iron-sulfur 3 (3Fe-4S).
FT   METAL       208    208       Iron-sulfur 3 (3Fe-4S).
FT   METAL       214    214       Iron-sulfur 3 (3Fe-4S).
FT   METAL       218    218       Iron-sulfur 2 (4Fe-4S).
FT   STRAND        4     11       {ECO:0000244|PDB:2BS2}.
FT   STRAND       22     29       {ECO:0000244|PDB:2BS2}.
FT   HELIX        36     46       {ECO:0000244|PDB:2BS2}.
FT   STRAND       56     62       {ECO:0000244|PDB:2BS2}.
FT   STRAND       66     69       {ECO:0000244|PDB:2BS2}.
FT   STRAND       72     75       {ECO:0000244|PDB:2BS2}.
FT   HELIX        76     78       {ECO:0000244|PDB:2BS2}.
FT   HELIX        81     83       {ECO:0000244|PDB:2BS2}.
FT   STRAND       87     92       {ECO:0000244|PDB:2BS2}.
FT   STRAND       97    101       {ECO:0000244|PDB:2BS2}.
FT   STRAND      104    106       {ECO:0000244|PDB:2BS2}.
FT   HELIX       108    117       {ECO:0000244|PDB:2BS2}.
FT   STRAND      130    132       {ECO:0000244|PDB:1E7P}.
FT   HELIX       139    149       {ECO:0000244|PDB:2BS2}.
FT   HELIX       156    159       {ECO:0000244|PDB:2BS2}.
FT   HELIX       162    166       {ECO:0000244|PDB:2BS2}.
FT   HELIX       172    183       {ECO:0000244|PDB:2BS2}.
FT   HELIX       192    199       {ECO:0000244|PDB:2BS2}.
FT   TURN        202    204       {ECO:0000244|PDB:2BS2}.
FT   HELIX       205    207       {ECO:0000244|PDB:2BS2}.
FT   HELIX       213    217       {ECO:0000244|PDB:2BS2}.
FT   HELIX       224    235       {ECO:0000244|PDB:2BS2}.
SQ   SEQUENCE   239 AA;  27165 MW;  24D1992489B8FFD1 CRC64;
     MGRMLTIRVF KYDPQSAVSK PHFQEYKIEE APSMTIFIVL NMIRETYDPD LNFDFVCRAG
     ICGSCGMMIN GRPSLACRTL TKDFEDGVIT LLPLPAFKLI KDLSVDTGNW FNGMSQRVES
     WIHAQKEHDI SKLEERIEPE VAQEVFELDR CIECGCCIAA CGTKIMREDF VGAAGLNRVV
     RFMIDPHDER TDEDYYELIG DDDGVFGCMT LLACHDVCPK NLPLQSKIAY LRRKMVSVN
//
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