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Database: UniProt
Entry: P20703
LinkDB: P20703
Original site: P20703 
ID   UVSW_BPT4               Reviewed;         587 AA.
AC   P20703;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   18-SEP-2019, entry version 110.
DE   RecName: Full=ATP-dependent DNA helicase uvsW;
DE            EC=3.6.4.12;
DE   AltName: Full=Dar protein;
GN   Name=uvsW; Synonyms=dar;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS, AND CHARACTERIZATION.
RX   PubMed=9233823; DOI=10.1093/emboj/16.13.4142;
RA   Carles-Kinch K., George J.W., Kreuzer K.N.;
RT   "Bacteriophage T4 UvsW protein is a helicase involved in
RT   recombination, repair and the regulation of DNA replication origins.";
RL   EMBO J. 16:4142-4151(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-139.
RX   PubMed=2377482; DOI=10.1093/nar/18.14.4277;
RA   Kaliman A.V., Khasanova M.A., Kryukov V.M., Tanyashin V.I.,
RA   Bayev A.A.;
RT   "The nucleotide sequence of the region of bacteriophage T4 inh(lip)-
RT   hoc genes.";
RL   Nucleic Acids Res. 18:4277-4277(1990).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15.
RX   PubMed=2388264; DOI=10.1016/0022-2836(90)90283-r;
RA   Derr L.K., Kreuzer K.N.;
RT   "Expression and function of the uvsW gene of bacteriophage T4.";
RL   J. Mol. Biol. 214:643-656(1990).
RN   [5]
RP   FUNCTION.
RX   PubMed=24013402; DOI=10.1038/ncomms3368;
RA   Manosas M., Perumal S.K., Bianco P., Ritort F., Benkovic S.J.,
RA   Croquette V.;
RT   "RecG and UvsW catalyse robust DNA rewinding critical for stalled DNA
RT   replication fork rescue.";
RL   Nat. Commun. 4:2368-2368(2013).
RN   [6]
RP   FUNCTION, AND INTERACTION WITH GP32.
RX   PubMed=23732982; DOI=10.1016/j.jmb.2013.05.012;
RA   Perumal S.K., Nelson S.W., Benkovic S.J.;
RT   "Interaction of T4 UvsW helicase and single-stranded DNA binding
RT   protein gp32 through its carboxy-terminal acidic tail.";
RL   J. Mol. Biol. 425:2823-2839(2013).
RN   [7]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-282.
RX   PubMed=15062081; DOI=10.1016/j.str.2004.02.016;
RA   Sickmier E.A., Kreuzer K.N., White S.W.;
RT   "The crystal structure of the UvsW helicase from bacteriophage T4.";
RL   Structure 12:583-592(2004).
CC   -!- FUNCTION: Plays important roles in recombination-dependent DNA
CC       repair and the reorganization of stalled replication forks during
CC       viral DNA synthesis. {ECO:0000269|PubMed:23732982,
CC       ECO:0000269|PubMed:24013402}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBUNIT: Interacts with gp32. {ECO:0000269|PubMed:23732982}.
DR   EMBL; AF158101; AAD42668.1; -; Genomic_DNA.
DR   EMBL; X56097; CAA39576.1; -; Genomic_DNA.
DR   EMBL; X14869; CAA33014.1; -; Genomic_DNA.
DR   PIR; PQ0097; S10498.
DR   RefSeq; NP_049796.1; NC_000866.4.
DR   PDB; 1RIF; X-ray; 2.00 A; A/B=1-282.
DR   PDB; 2JPN; NMR; -; A=512-587.
DR   PDB; 2OCA; X-ray; 2.70 A; A=1-502.
DR   PDBsum; 1RIF; -.
DR   PDBsum; 2JPN; -.
DR   PDBsum; 2OCA; -.
DR   SMR; P20703; -.
DR   GeneID; 1258733; -.
DR   KEGG; vg:1258733; -.
DR   KO; K18959; -.
DR   EvolutionaryTrace; P20703; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   Gene3D; 1.20.1280.210; -; 1.
DR   InterPro; IPR020975; DNA-helicase_ATP-dep_UvsW.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038341; UvsW_sf.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   Pfam; PF11637; UvsW; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Late protein; Nucleotide-binding; Reference proteome.
FT   CHAIN         1    587       ATP-dependent DNA helicase uvsW.
FT                                /FTId=PRO_0000164988.
FT   DOMAIN      122    280       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      335    501       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     135    142       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MUTAGEN     141    141       K->R: Loss of activity.
FT                                {ECO:0000269|PubMed:9233823}.
FT   STRAND        3      8       {ECO:0000244|PDB:1RIF}.
FT   TURN          9     11       {ECO:0000244|PDB:1RIF}.
FT   STRAND       12     16       {ECO:0000244|PDB:1RIF}.
FT   HELIX        19     28       {ECO:0000244|PDB:1RIF}.
FT   STRAND       30     32       {ECO:0000244|PDB:1RIF}.
FT   TURN         34     37       {ECO:0000244|PDB:1RIF}.
FT   HELIX        40     43       {ECO:0000244|PDB:1RIF}.
FT   STRAND       49     54       {ECO:0000244|PDB:1RIF}.
FT   STRAND       59     61       {ECO:0000244|PDB:1RIF}.
FT   HELIX        62     67       {ECO:0000244|PDB:1RIF}.
FT   HELIX        68     74       {ECO:0000244|PDB:1RIF}.
FT   STRAND       79     81       {ECO:0000244|PDB:1RIF}.
FT   HELIX        83     86       {ECO:0000244|PDB:1RIF}.
FT   HELIX        93    101       {ECO:0000244|PDB:1RIF}.
FT   STRAND      105    107       {ECO:0000244|PDB:2OCA}.
FT   STRAND      110    112       {ECO:0000244|PDB:2OCA}.
FT   HELIX       116    128       {ECO:0000244|PDB:1RIF}.
FT   STRAND      129    133       {ECO:0000244|PDB:1RIF}.
FT   TURN        138    140       {ECO:0000244|PDB:2OCA}.
FT   HELIX       141    155       {ECO:0000244|PDB:1RIF}.
FT   STRAND      157    163       {ECO:0000244|PDB:1RIF}.
FT   HELIX       167    179       {ECO:0000244|PDB:1RIF}.
FT   HELIX       185    187       {ECO:0000244|PDB:1RIF}.
FT   STRAND      188    190       {ECO:0000244|PDB:1RIF}.
FT   HELIX       192    194       {ECO:0000244|PDB:2OCA}.
FT   HELIX       199    201       {ECO:0000244|PDB:2OCA}.
FT   STRAND      206    210       {ECO:0000244|PDB:1RIF}.
FT   HELIX       212    215       {ECO:0000244|PDB:1RIF}.
FT   HELIX       220    225       {ECO:0000244|PDB:1RIF}.
FT   STRAND      226    232       {ECO:0000244|PDB:1RIF}.
FT   HELIX       234    236       {ECO:0000244|PDB:1RIF}.
FT   HELIX       239    245       {ECO:0000244|PDB:1RIF}.
FT   TURN        246    248       {ECO:0000244|PDB:1RIF}.
FT   STRAND      254    258       {ECO:0000244|PDB:1RIF}.
FT   HELIX       262    264       {ECO:0000244|PDB:2OCA}.
FT   HELIX       269    276       {ECO:0000244|PDB:1RIF}.
FT   STRAND      278    280       {ECO:0000244|PDB:1RIF}.
FT   STRAND      298    305       {ECO:0000244|PDB:2OCA}.
FT   HELIX       308    314       {ECO:0000244|PDB:2OCA}.
FT   HELIX       319    327       {ECO:0000244|PDB:2OCA}.
FT   HELIX       330    344       {ECO:0000244|PDB:2OCA}.
FT   TURN        345    347       {ECO:0000244|PDB:2OCA}.
FT   STRAND      349    356       {ECO:0000244|PDB:2OCA}.
FT   HELIX       357    368       {ECO:0000244|PDB:2OCA}.
FT   STRAND      372    380       {ECO:0000244|PDB:2OCA}.
FT   HELIX       383    395       {ECO:0000244|PDB:2OCA}.
FT   STRAND      400    404       {ECO:0000244|PDB:2OCA}.
FT   HELIX       405    410       {ECO:0000244|PDB:2OCA}.
FT   STRAND      416    424       {ECO:0000244|PDB:2OCA}.
FT   HELIX       431    440       {ECO:0000244|PDB:2OCA}.
FT   STRAND      450    457       {ECO:0000244|PDB:2OCA}.
FT   STRAND      463    466       {ECO:0000244|PDB:2OCA}.
FT   HELIX       476    490       {ECO:0000244|PDB:2OCA}.
FT   STRAND      495    501       {ECO:0000244|PDB:2OCA}.
FT   STRAND      514    517       {ECO:0000244|PDB:2JPN}.
FT   HELIX       518    523       {ECO:0000244|PDB:2JPN}.
FT   HELIX       528    533       {ECO:0000244|PDB:2JPN}.
FT   HELIX       538    551       {ECO:0000244|PDB:2JPN}.
FT   TURN        552    554       {ECO:0000244|PDB:2JPN}.
FT   HELIX       560    578       {ECO:0000244|PDB:2JPN}.
FT   TURN        583    585       {ECO:0000244|PDB:2JPN}.
SQ   SEQUENCE   587 AA;  67525 MW;  D7FBD8CE1E38C7CA CRC64;
     MDIKVHFHDF SHVRIDCEES TFHELRDFFS FEADGYRFNP RFRYGNWDGR IRLLDYNRLL
     PFGLVGQIKK FCDNFGYKAW IDPQINEKEE LSRKDFDEWL SKLEIYSGNK RIEPHWYQKD
     AVFEGLVNRR RILNLPTSAG KSLIQALLAR YYLENYEGKI LIIVPTTALT TQMADDFVDY
     RLFSHAMIKK IGGGASKDDK YKNDAPVVVG TWQTVVKQPK EWFSQFGMMM NDECHLATGK
     SISSIISGLN NCMFKFGLSG SLRDGKANIM QYVGMFGEIF KPVTTSKLME DGQVTELKIN
     SIFLRYPDEF TTKLKGKTYQ EEIKIITGLS KRNKWIAKLA IKLAQKDENA FVMFKHVSHG
     KAIFDLIKNE YDKVYYVSGE VDTETRNIMK TLAENGKGII IVASYGVFST GISVKNLHHV
     VLAHGVKSKI IVLQTIGRVL RKHGSKTIAT VWDLIDSAGV KPKSANTKKK YVHLNYLLKH
     GIDRIQRYAD EKFNYVMKTV NLISFGPLEK KMLLEFKQFL YEASIDEFMG KIASCQTLEG
     LEELEAYYKK RVKETELKDT DDISVRDALA GKRAELEDSD DEVEESF
//
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