ID VLP7_BORHE Reviewed; 369 AA.
AC P21876; Q3S4W5;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1991, sequence version 1.
DT 28-JUN-2023, entry version 85.
DE RecName: Full=Variable large protein 7 {ECO:0000303|PubMed:9453591};
DE AltName: Full=Variable major outer membrane lipoprotein 7 {ECO:0000303|PubMed:1706456};
DE Flags: Precursor;
GN Name=vlp7 {ECO:0000303|PubMed:9453591};
GN Synonyms=vmp7 {ECO:0000303|PubMed:1706456};
OS Borrelia hermsii.
OG Plasmid bp7E, Plasmid lp32-1, and Plasmid lp7E.
OC Bacteria; Spirochaetota; Spirochaetia; Spirochaetales; Borreliaceae;
OC Borrelia.
OX NCBI_TaxID=140;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 35209 / HS1; PLASMID=bp7E;
RX PubMed=1706456; DOI=10.1111/j.1365-2958.1990.tb00549.x;
RA Burman N., Bergstroem S., Restrepo B.I., Barbour A.G.;
RT "The variable antigens Vmp7 and Vmp21 of the relapsing fever bacterium
RT Borrelia hermsii are structurally analogous to the VSG proteins of the
RT African trypanosome.";
RL Mol. Microbiol. 4:1715-1726(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 35209 / HS1; PLASMID=bp7E;
RX PubMed=1987053; DOI=10.1128/iai.59.1.390-397.1991;
RA Barbour A.G., Carter C.J., Burman N., Freitag C.S., Garon C.F.,
RA Bergstrom S.;
RT "Tandem insertion sequence-like elements define the expression site for
RT variable antigen genes of Borrelia hermsii.";
RL Infect. Immun. 59:390-397(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 35209 / HS1; PLASMID=lp7E;
RX PubMed=1484486; DOI=10.1111/j.1365-2958.1992.tb02198.x;
RA Restrepo B.I., Kitten T., Carter C.J., Infante D., Barbour A.G.;
RT "Subtelomeric expression regions of Borrelia hermsii linear plasmids are
RT highly polymorphic.";
RL Mol. Microbiol. 6:3299-3311(1992).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 35209 / HS1; PLASMID=lp32-1;
RA Dai Q., Restrepo B.I., Porcella S.F., Schwan T.G., Barbour A.G.;
RT "Antigenic variation during relapsing fever through recombination between
RT extragenic sequences.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PROTEIN SEQUENCE OF 52-72 AND 244-258, AND SUBCELLULAR LOCATION.
RX PubMed=2409197; DOI=10.1084/jem.161.6.1302;
RA Barstad P.A., Coligan J.E., Raum M.G., Barbour A.G.;
RT "Variable major proteins of Borrelia hermsii. Epitope mapping and partial
RT sequence analysis of CNBr peptides.";
RL J. Exp. Med. 161:1302-1314(1985).
RN [6]
RP FUNCTION.
RC STRAIN=ATCC 35209 / HS1; PLASMID=bp7E;
RX PubMed=2385585; DOI=10.1073/pnas.87.16.6077;
RA Kitten T., Barbour A.G.;
RT "Juxtaposition of expressed variable antigen genes with a conserved
RT telomere in the bacterium Borrelia hermsii.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:6077-6081(1990).
RN [7]
RP NOMENCLATURE.
RX PubMed=9453591; DOI=10.1128/iai.66.2.432-440.1998;
RA Hinnebusch B.J., Barbour A.G., Restrepo B.I., Schwan T.G.;
RT "Population structure of the relapsing fever spirochete Borrelia hermsii as
RT indicated by polymorphism of two multigene families that encode immunogenic
RT outer surface lipoproteins.";
RL Infect. Immun. 66:432-440(1998).
RN [8]
RP FUNCTION.
RX PubMed=11159997; DOI=10.1128/iai.69.2.1009-1015.2001;
RA Barbour A.G., Bundoc V.;
RT "In vitro and in vivo neutralization of the relapsing fever agent Borrelia
RT hermsii with serotype-specific immunoglobulin M antibodies.";
RL Infect. Immun. 69:1009-1015(2001).
CC -!- FUNCTION: The Vlp and Vsp proteins are antigenically distinct proteins,
CC only one vlp or vsp gene is transcriptionally active at any one time.
CC Switching between these genes is a mechanism of host immune response
CC evasion. {ECO:0000269|PubMed:11159997, ECO:0000269|PubMed:2385585}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305|PubMed:2409197};
CC Lipid-anchor.
CC -!- MISCELLANEOUS: Genes for both Vlp and Vsp families are on (usually)
CC unnamed linear plasmids in B.hermsii HS1. {ECO:0000305|PubMed:9453591}.
CC -!- SIMILARITY: Belongs to the variable large protein (Vlp) family. Alpha
CC subfamily. {ECO:0000305}.
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DR EMBL; X53926; CAA37873.1; -; Genomic_DNA.
DR EMBL; DQ218042; ABC55440.1; -; Genomic_DNA.
DR EMBL; DQ166207; AAZ94629.1; -; Genomic_DNA.
DR PIR; A43579; A43579.
DR PIR; S11980; S11980.
DR RefSeq; WP_015633304.1; NZ_CP014812.1.
DR AlphaFoldDB; P21876; -.
DR SMR; P21876; -.
DR OrthoDB; 351090at2; -.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR000680; Borrelia_lipo.
DR Pfam; PF00921; Lipoprotein_2; 1.
DR SUPFAM; SSF74748; Variable surface antigen VlsE; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW Cell outer membrane; Direct protein sequencing; Lipoprotein; Membrane;
KW Palmitate; Plasmid; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000305"
FT CHAIN 27..369
FT /note="Variable large protein 7"
FT /id="PRO_0000018087"
FT LIPID 27
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000305"
FT LIPID 27
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000305"
SQ SEQUENCE 369 AA; 37102 MW; F318727E39399DA8 CRC64;
MRKRISAIIN KLNISIIIMT VVLMIGCGQQ PEAGKTGVSG GVNGNLGNSL MELGRSAENA
FYAFIELVSD VLGFTAKSDT TKQEVGGYFN SLGAKLGEAS NDLEQVAVKA ETGVDKSDSS
KNPIREAVNE AKEVLGTLKG YVESLGTIGD SNPVGYANNA AGSGTTAADD ELRKAFKALQ
EIVKAATDAG VKALKIGATT LQANGGADNK EGAKILATSG GNPAAADVAK AAAILSSVSG
EEMLSSIVKS GENDAQLAAA ADGNTSAISF AKGGSDAHLA GANTPKAAAV AGGIALRSLV
KTGKLAAGAA DNATGGGKEV QGVGVAAANK LLRAVEDVIK KTVKNVLEKA KEKIDKARGS
QEPVSESSK
//
