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Database: UniProt
Entry: P27639
LinkDB: P27639
Original site: P27639 
ID   IF4A_CAEEL              Reviewed;         402 AA.
AC   P27639; Q4TTC4;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   16-OCT-2019, entry version 156.
DE   RecName: Full=Eukaryotic initiation factor 4A;
DE            Short=eIF-4A;
DE            EC=3.6.4.13;
DE   AltName: Full=ATP-dependent RNA helicase eIF4A;
DE   AltName: Full=Initiation factor 1;
GN   Name=inf-1; ORFNames=F57B9.6;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
RC   STRAIN=Bristol N2;
RX   PubMed=1641346; DOI=10.1093/nar/20.14.3783;
RA   Roussell D.L., Bennett K.L.;
RT   "Caenorhabditis cDNA encodes an eIF-4A-like protein.";
RL   Nucleic Acids Res. 20:3783-3783(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE
RP   SPLICING.
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: ATP-dependent RNA helicase which is a subunit of the
CC       eIF4F complex involved in cap recognition and is required for mRNA
CC       binding to ribosome. In the current model of translation
CC       initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR
CC       of mRNAs which is necessary to allow efficient binding of the
CC       small ribosomal subunit, and subsequent scanning for the initiator
CC       codon.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: eIF4F is a multi-subunit complex, the composition of
CC       which varies with external and internal environmental conditions.
CC       It is composed of at least EIF4A, EIF4E and EIF4G (By similarity).
CC       {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=a;
CC         IsoId=P27639-1; Sequence=Displayed;
CC       Name=b;
CC         IsoId=P27639-2; Sequence=VSP_020640;
CC         Note=No experimental confirmation available.;
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. eIF4A
CC       subfamily. {ECO:0000305}.
DR   EMBL; Z12116; CAA78102.1; -; mRNA.
DR   EMBL; FO081266; CCD70313.1; -; Genomic_DNA.
DR   EMBL; FO081266; CCD70314.1; -; Genomic_DNA.
DR   PIR; S26281; S26281.
DR   RefSeq; NP_001022623.1; NM_001027452.2. [P27639-1]
DR   RefSeq; NP_001022624.1; NM_001027453.2. [P27639-2]
DR   SMR; P27639; -.
DR   BioGrid; 41180; 8.
DR   DIP; DIP-27102N; -.
DR   IntAct; P27639; 1.
DR   STRING; 6239.F57B9.6a.2; -.
DR   EPD; P27639; -.
DR   PaxDb; P27639; -.
DR   PeptideAtlas; P27639; -.
DR   PRIDE; P27639; -.
DR   EnsemblMetazoa; F57B9.6a.1; F57B9.6a.1; WBGene00002083. [P27639-1]
DR   EnsemblMetazoa; F57B9.6a.2; F57B9.6a.2; WBGene00002083. [P27639-1]
DR   EnsemblMetazoa; F57B9.6a.3; F57B9.6a.3; WBGene00002083. [P27639-1]
DR   EnsemblMetazoa; F57B9.6b.1; F57B9.6b.1; WBGene00002083. [P27639-2]
DR   EnsemblMetazoa; F57B9.6b.2; F57B9.6b.2; WBGene00002083. [P27639-2]
DR   EnsemblMetazoa; F57B9.6b.3; F57B9.6b.3; WBGene00002083. [P27639-2]
DR   GeneID; 175966; -.
DR   KEGG; cel:CELE_F57B9.6; -.
DR   UCSC; F57B9.6a.1; c. elegans. [P27639-1]
DR   CTD; 175966; -.
DR   WormBase; F57B9.6a; CE01341; WBGene00002083; inf-1. [P27639-1]
DR   WormBase; F57B9.6b; CE38524; WBGene00002083; inf-1. [P27639-2]
DR   eggNOG; KOG0327; Eukaryota.
DR   eggNOG; COG0513; LUCA.
DR   GeneTree; ENSGT00940000153889; -.
DR   HOGENOM; HOG000268797; -.
DR   InParanoid; P27639; -.
DR   KO; K03257; -.
DR   OMA; RNPIRIL; -.
DR   OrthoDB; 726081at2759; -.
DR   PhylomeDB; P27639; -.
DR   Reactome; R-CEL-1169408; ISG15 antiviral mechanism.
DR   Reactome; R-CEL-156827; L13a-mediated translational silencing of Ceruloplasmin expression.
DR   Reactome; R-CEL-72649; Translation initiation complex formation.
DR   Reactome; R-CEL-72662; Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
DR   Reactome; R-CEL-72702; Ribosomal scanning and start codon recognition.
DR   PRO; PR:P27639; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00002083; Expressed in 6 organ(s), highest expression level in multi-cellular organism.
DR   GO; GO:0010494; C:cytoplasmic stress granule; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR   GO; GO:0002183; P:cytoplasmic translational initiation; IBA:GO_Central.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; ATP-binding; Complete proteome; Helicase;
KW   Hydrolase; Initiation factor; Nucleotide-binding;
KW   Protein biosynthesis; Reference proteome; RNA-binding.
FT   CHAIN         1    402       Eukaryotic initiation factor 4A.
FT                                /FTId=PRO_0000054945.
FT   DOMAIN       60    230       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      241    402       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND      73     80       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF        29     57       Q motif.
FT   MOTIF       178    181       DEAD box.
FT   VAR_SEQ       1    287       Missing (in isoform b). {ECO:0000305}.
FT                                /FTId=VSP_020640.
SQ   SEQUENCE   402 AA;  45408 MW;  AB1CE281F9FC8881 CRC64;
     MTDVKNDVNV SSVVDADGLI EGNYDQVVES FDDMELKEEL LRGIYGFGFE KPSAIQKRAI
     VPCTTGKDVI AQAQSGTGKT ATFSVSILQR IDHEDPHVQA LVMAPTRELA QQIQKVMSAL
     GEYLNVNILP CIGGTSVRDD QRKLEAGIHV VVGTPGRVGD MINRNALDTS RIKMFVLDEA
     DEMLSRGFKD QIYEVFRSMP QDVQVVLLSA TMPSEVLDVT NRFMRNPIRI LVKKDELTLE
     GIRQFYINVQ KDEWKFDCLC DLYNVVNVTQ AVIFCNTRRK VDTLTEKMTE NQFTVSCLHG
     DMDQAERDTI MREFRSGSSR VLITTDILAR GIDVQQVSLV INYDLPSNRE NYIHRIGRSG
     RFGRKGVAIN FVTENDARQL KEIESYYTTQ IEEMPESIAD LI
//
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