ID GBRG3_RAT Reviewed; 467 AA.
AC P28473;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 24-JAN-2024, entry version 170.
DE RecName: Full=Gamma-aminobutyric acid receptor subunit gamma-3;
DE AltName: Full=GABA(A) receptor subunit gamma-3;
DE Flags: Precursor;
GN Name=Gabrg3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=1311098; DOI=10.1073/pnas.89.4.1433;
RA Herb A., Wisden W., Lueddens H., Puia G., Vicini S., Seeburg P.H.;
RT "The third gamma subunit of the gamma-aminobutyric acid type A receptor
RT family.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:1433-1437(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=ZUR-SIV; TISSUE=Brain;
RX PubMed=1660002; DOI=10.1016/0014-5793(91)81184-a;
RA Knoflach F., Rhyner T., Villa M., Kellenberger S., Drescher U.,
RA Malherbe P., Sigel E., Moehler H.;
RT "The gamma 3-subunit of the GABAA-receptor confers sensitivity to
RT benzodiazepine receptor ligands.";
RL FEBS Lett. 293:191-194(1991).
CC -!- FUNCTION: GABA, the major inhibitory neurotransmitter in the vertebrate
CC brain, mediates neuronal inhibition by binding to the
CC GABA/benzodiazepine receptor and opening an integral chloride channel.
CC -!- SUBUNIT: Generally pentameric. There are five types of GABA(A) receptor
CC chains: alpha, beta, gamma, delta, and rho.
CC -!- SUBCELLULAR LOCATION: Postsynaptic cell membrane; Multi-pass membrane
CC protein. Cell membrane; Multi-pass membrane protein.
CC -!- PTM: May be palmitoylated. {ECO:0000250}.
CC -!- MISCELLANEOUS: This subunit carries the benzodiazepine binding site.
CC -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG3 sub-
CC subfamily. {ECO:0000305}.
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DR EMBL; M81142; AAA41181.1; -; mRNA.
DR EMBL; X63324; CAA44930.1; -; mRNA.
DR PIR; S19317; S19317.
DR RefSeq; NP_077346.3; NM_024370.3.
DR AlphaFoldDB; P28473; -.
DR SMR; P28473; -.
DR STRING; 10116.ENSRNOP00000076184; -.
DR ChEMBL; CHEMBL1907607; -.
DR DrugCentral; P28473; -.
DR GlyCosmos; P28473; 2 sites, No reported glycans.
DR GlyGen; P28473; 2 sites.
DR PhosphoSitePlus; P28473; -.
DR PaxDb; 10116-ENSRNOP00000019971; -.
DR Ensembl; ENSRNOT00000093339.2; ENSRNOP00000076184.1; ENSRNOG00000014862.8.
DR GeneID; 79211; -.
DR KEGG; rno:79211; -.
DR UCSC; RGD:621735; rat.
DR AGR; RGD:621735; -.
DR CTD; 2567; -.
DR RGD; 621735; Gabrg3.
DR eggNOG; KOG3642; Eukaryota.
DR GeneTree; ENSGT00940000155607; -.
DR InParanoid; P28473; -.
DR OMA; MYWQELE; -.
DR OrthoDB; 4265336at2759; -.
DR PhylomeDB; P28473; -.
DR TreeFam; TF315453; -.
DR Reactome; R-RNO-977443; GABA receptor activation.
DR PRO; PR:P28473; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000014862; Expressed in frontal cortex and 2 other cell types or tissues.
DR ExpressionAtlas; P28473; baseline and differential.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0032590; C:dendrite membrane; IBA:GO_Central.
DR GO; GO:1902711; C:GABA-A receptor complex; IBA:GO_Central.
DR GO; GO:0098982; C:GABA-ergic synapse; ISO:RGD.
DR GO; GO:0015630; C:microtubule cytoskeleton; IEA:Ensembl.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IEA:Ensembl.
DR GO; GO:0098794; C:postsynapse; IBA:GO_Central.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0097060; C:synaptic membrane; ISO:RGD.
DR GO; GO:0005254; F:chloride channel activity; IDA:RGD.
DR GO; GO:0004890; F:GABA-A receptor activity; IDA:RGD.
DR GO; GO:0022851; F:GABA-gated chloride ion channel activity; IBA:GO_Central.
DR GO; GO:0005237; F:inhibitory extracellular ligand-gated monoatomic ion channel activity; IBA:GO_Central.
DR GO; GO:1904315; F:transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential; ISO:RGD.
DR GO; GO:1902476; P:chloride transmembrane transport; IBA:GO_Central.
DR GO; GO:0007214; P:gamma-aminobutyric acid signaling pathway; IBA:GO_Central.
DR GO; GO:0060078; P:regulation of postsynaptic membrane potential; IBA:GO_Central.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IDA:RGD.
DR GO; GO:0051932; P:synaptic transmission, GABAergic; IBA:GO_Central.
DR CDD; cd19000; LGIC_ECD_GABAAR_G; 1.
DR CDD; cd19054; LGIC_TM_GABAAR_gamma; 1.
DR Gene3D; 2.70.170.10; Neurotransmitter-gated ion-channel ligand-binding domain; 1.
DR Gene3D; 1.20.58.390; Neurotransmitter-gated ion-channel transmembrane domain; 1.
DR InterPro; IPR006028; GABAA/Glycine_rcpt.
DR InterPro; IPR005440; GABBAg3_rcpt.
DR InterPro; IPR005437; GABRG-1/4.
DR InterPro; IPR006202; Neur_chan_lig-bd.
DR InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR InterPro; IPR006201; Neur_channel.
DR InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR InterPro; IPR038050; Neuro_actylchol_rec.
DR InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR NCBIfam; TIGR00860; LIC; 1.
DR PANTHER; PTHR18945:SF195; GAMMA-AMINOBUTYRIC ACID RECEPTOR SUBUNIT GAMMA-3; 1.
DR PANTHER; PTHR18945; NEUROTRANSMITTER GATED ION CHANNEL; 1.
DR Pfam; PF02931; Neur_chan_LBD; 1.
DR Pfam; PF02932; Neur_chan_memb; 1.
DR PRINTS; PR00253; GABAARECEPTR.
DR PRINTS; PR01620; GABAARGAMMA.
DR PRINTS; PR01623; GABAARGAMMA3.
DR PRINTS; PR00252; NRIONCHANNEL.
DR SUPFAM; SSF90112; Neurotransmitter-gated ion-channel transmembrane pore; 1.
DR SUPFAM; SSF63712; Nicotinic receptor ligand binding domain-like; 1.
DR PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Chloride; Chloride channel; Disulfide bond; Glycoprotein;
KW Ion channel; Ion transport; Lipoprotein; Membrane; Palmitate;
KW Postsynaptic cell membrane; Reference proteome; Signal; Synapse;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..467
FT /note="Gamma-aminobutyric acid receptor subunit gamma-3"
FT /id="PRO_0000000483"
FT TOPO_DOM 18..254
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 255..277
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TRANSMEM 281..303
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TRANSMEM 315..337
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 338..443
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 444..467
FT /note="Helical"
FT /evidence="ECO:0000305"
FT CARBOHYD 110
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 228
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 171..185
FT /evidence="ECO:0000250"
FT CONFLICT 416
FT /note="S -> T (in Ref. 2; CAA44930)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 467 AA; 54293 MW; 14959F565649D67D CRC64;
MAAKLLLLLC LFSGLHARSR RVEEDDSEDS PSNQKWVLAP KSQDTDVTLI LNKLLREYDK
KLRPDIGIKP TVIDVDIYVN SIGPVSSINM EYQIDIFFAQ TWTDSRLRFN STMKILTLNS
NMVGLIWIPD TIFRNSKTAE AHWITTPNQL LRIWNDGKIL YTLRLTINAE CQLQLHNFPM
DAHACPLTFS SYGYPKEEMI YRWRKNSVEA ADQKSWRLYQ FDFMGLRNTT EIVTTSAGDY
VVMTIYFELS RRMGYFTIQT YIPCILTVVL SWVSFWIKKD ATPARTTLGI TTVLTMTTLS
TIARKSLPRV SYVTAMDLFV TVCFLFVFAA LMEYATLNYY SSCRKPTIRK KKTSLLHPDS
TRWIPDRISL QAPSNYSLLD MRPPPPVMIT LNNSMYWQEF EDTCVYECLD GKDCQSFFCC
YEECKSGSWR RGRIHIDVSE LDSYSRVFFP TSFLLFNLVY WVGYLYL
//
