ID P2C48_ORYSJ Reviewed; 353 AA.
AC Q6L482; A0A0N7KKM0;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 24-JAN-2024, entry version 112.
DE RecName: Full=Probable protein phosphatase 2C 48;
DE Short=OsPP2C48;
DE EC=3.1.3.16;
GN OrderedLocusNames=Os05g0358500, LOC_Os05g29030;
GN ORFNames=OJ1045_C06.13, OSJNBa0009L15.3;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19021904; DOI=10.1186/1471-2164-9-550;
RA Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.;
RT "Genome-wide and expression analysis of protein phosphatase 2C in rice and
RT Arabidopsis.";
RL BMC Genomics 9:550-550(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:83421; EC=3.1.3.16;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:61977; EC=3.1.3.16;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000250};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6L482-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6L482-2; Sequence=VSP_036270;
CC -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AK100777; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC104272; AAV43855.1; -; Genomic_DNA.
DR EMBL; AC136999; AAT38039.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF17217.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS93593.1; -; Genomic_DNA.
DR EMBL; AK100777; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015639910.1; XM_015784424.1.
DR RefSeq; XP_015639911.1; XM_015784425.1.
DR AlphaFoldDB; Q6L482; -.
DR SMR; Q6L482; -.
DR STRING; 39947.Q6L482; -.
DR PaxDb; 39947-Q6L482; -.
DR EnsemblPlants; Os05t0358500-01; Os05t0358500-01; Os05g0358500. [Q6L482-1]
DR EnsemblPlants; Os05t0358500-02; Os05t0358500-02; Os05g0358500. [Q6L482-1]
DR GeneID; 4338531; -.
DR Gramene; Os05t0358500-01; Os05t0358500-01; Os05g0358500. [Q6L482-1]
DR Gramene; Os05t0358500-02; Os05t0358500-02; Os05g0358500. [Q6L482-1]
DR KEGG; osa:4338531; -.
DR eggNOG; KOG0698; Eukaryota.
DR HOGENOM; CLU_013173_6_0_1; -.
DR InParanoid; Q6L482; -.
DR OMA; HSYIKTC; -.
DR OrthoDB; 1450097at2759; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR Genevisible; Q6L482; OS.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0004722; F:protein serine/threonine phosphatase activity; IBA:GO_Central.
DR CDD; cd00143; PP2Cc; 1.
DR Gene3D; 3.60.40.10; PPM-type phosphatase domain; 1.
DR InterPro; IPR015655; PP2C.
DR InterPro; IPR036457; PPM-type-like_dom_sf.
DR InterPro; IPR001932; PPM-type_phosphatase-like_dom.
DR PANTHER; PTHR47992; PROTEIN PHOSPHATASE; 1.
DR PANTHER; PTHR47992:SF48; PROTEIN PHOSPHATASE 2C 34-RELATED; 1.
DR Pfam; PF00481; PP2C; 1.
DR SMART; SM00332; PP2Cc; 1.
DR SUPFAM; SSF81606; PP2C-like; 1.
DR PROSITE; PS51746; PPM_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Hydrolase; Magnesium; Manganese; Metal-binding;
KW Protein phosphatase; Reference proteome.
FT CHAIN 1..353
FT /note="Probable protein phosphatase 2C 48"
FT /id="PRO_0000363295"
FT DOMAIN 54..348
FT /note="PPM-type phosphatase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT BINDING 90
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 90
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 91
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 293
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 339
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT VAR_SEQ 295..353
FT /note="VWDVVSNEEAVQIVASAPEREKAAKRLVEFAVRAWRRKRRGIAVDDCSAICL
FT FFHSPPS -> DKHLNLFVFVCAAGVGRGVERGGGADRGVGAGEGEGGEAARRVRRPGM
FT EAQAPGHRRRRLLGDLPLLPLAAVLNNTHADTHAANKNRTRRRQCRRRR (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036270"
SQ SEQUENCE 353 AA; 38101 MW; 08013ADEAE50D4C2 CRC64;
MRHISSLLQG LARSLSVGKE RKGGDGDDGK AAAATATAVL RTSGTLWGEG SETFAAVCSR
RGEKGINQDC SIVCEGFGCE EGSVLCGIFD GHGQWGHYVA KAVRESLPPA LLRRWREAVT
LAALIDGGEK RLCECRPDLW RQSYLAACAA VDAELRASRR LDAVHSGCTA LSLVKHGDLL
VVANVGDSRA VLATASPDDG GGARLAAVQL TVDFKPNLPQ ERERIMECNG RVQCLADEPG
VHRVWRPDRE GPGLAMSRAF GDYCVKDYGV ISAPEVTHRR ITAQDHFVIL ATDGVWDVVS
NEEAVQIVAS APEREKAAKR LVEFAVRAWR RKRRGIAVDD CSAICLFFHS PPS
//
