GenomeNet

Database: UniProt
Entry: P30015
LinkDB: P30015
Original site: P30015 
ID   LHR_ECOLI               Reviewed;        1538 AA.
AC   P30015;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 2.
DT   13-NOV-2019, entry version 145.
DE   RecName: Full=Probable ATP-dependent helicase lhr;
DE            EC=3.6.4.-;
DE   AltName: Full=Large helicase-related protein;
GN   Name=lhr; Synonyms=rhlF; OrderedLocusNames=b1653, JW1645;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=7559321; DOI=10.1128/jb.177.19.5393-5400.1995;
RA   Reuven N.B., Koonin E.V., Rudd K.E., Deutscher M.P.;
RT   "The gene for the longest known Escherichia coli protein is a member
RT   of helicase superfamily II.";
RL   J. Bacteriol. 177:5393-5400(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA   Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M.,
RA   Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K.,
RA   Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N.,
RA   Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J.,
RA   Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.;
RT   "A 570-kb DNA sequence of the Escherichia coli K-12 genome
RT   corresponding to the 28.0-40.1 min region on the linkage map.";
RL   DNA Res. 3:363-377(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-130.
RC   STRAIN=K12;
RX   PubMed=1460056;
RA   Huang S., Deutscher M.P.;
RT   "Sequence and transcriptional analysis of the Escherichia coli rnt
RT   gene encoding RNase T.";
RL   J. Biol. Chem. 267:25609-25613(1992).
CC   -!- SIMILARITY: Belongs to the helicase family. {ECO:0000305}.
DR   EMBL; L01622; AAC37009.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74725.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15419.1; -; Genomic_DNA.
DR   PIR; G64922; G64922.
DR   RefSeq; NP_416170.1; NC_000913.3.
DR   RefSeq; WP_001310858.1; NZ_LN832404.1.
DR   SMR; P30015; -.
DR   BioGrid; 4260268; 108.
DR   DIP; DIP-10097N; -.
DR   IntAct; P30015; 9.
DR   STRING; 511145.b1653; -.
DR   jPOST; P30015; -.
DR   PaxDb; P30015; -.
DR   PRIDE; P30015; -.
DR   EnsemblBacteria; AAC74725; AAC74725; b1653.
DR   EnsemblBacteria; BAA15419; BAA15419; BAA15419.
DR   GeneID; 946156; -.
DR   KEGG; ecj:JW1645; -.
DR   KEGG; eco:b1653; -.
DR   PATRIC; fig|1411691.4.peg.606; -.
DR   EchoBASE; EB1510; -.
DR   eggNOG; ENOG4107QRT; Bacteria.
DR   eggNOG; COG1201; LUCA.
DR   HOGENOM; HOG000238716; -.
DR   InParanoid; P30015; -.
DR   KO; K03724; -.
DR   PhylomeDB; P30015; -.
DR   BioCyc; EcoCyc:EG11548-MONOMER; -.
DR   BioCyc; ECOL316407:JW1645-MONOMER; -.
DR   PRO; PR:P30015; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR013701; DEAD/DEAH_assoc.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF08494; DEAD_assoc; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN         1   1538       Probable ATP-dependent helicase lhr.
FT                                /FTId=PRO_0000102193.
FT   DOMAIN       38    235       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      284    462       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND      51     58       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       179    182       DEVH box.
SQ   SEQUENCE   1538 AA;  169381 MW;  563172B5FE3D826E CRC64;
     MADNPDPSSL LPDVFSPATR DWFLRAFKQP TAVQPQTWHV AARSEHALVI APTGSGKTLA
     AFLYALDRLF REGGEDTREA HKRKTSRILY ISPIKALGTD VQRNLQIPLK GIADERRRRG
     ETEVNLRVGI RTGDTPAQER SKLTRNPPDI LITTPESLYL MLTSRARETL RGVETVIIDE
     VHAVAGSKRG AHLALSLERL DALLHTSAQR IGLSATVRSA SDVAAFLGGD RPVTVVNPPA
     MRHPQIRIVV PVANMDDVSS VASGTGEDSH AGREGSIWPY IETGILDEVL RHRSTIVFTN
     SRGLAEKLTA RLNELYAARL QRSPSIAVDA AHFESTSGAT SNRVQSSDVF IARSHHGSVS
     KEQRAITEQA LKSGELRCVV ATSSLELGID MGAVDLVIQV ATPLSVASGL QRIGRAGHQV
     GGVSKGLFFP RTRRDLVDSA VIVECMFAGR LENLTPPHNP LDVLAQQTVA AAAMDALQVD
     EWYSRVRRAA PWKDLPRRVF DATLDMLSGR YPSGDFSAFR PKLVWNRETG ILTARPGAQL
     LAVTSGGTIP DRGMYSVLLP EGEEKAGSRR VGELDEEMVY ESRVNDIITL GATSWRIQQI
     TRDQVIVTPA PGRSARLPFW RGEGNGRPAE LGEMIGDFLH LLADGAFFSG TIPPWLAEEN
     TIANIQGLIE EQRNATGIVP GSRHLVLERC RDEIGDWRII LHSPYGRRVH EPWAVAIAGR
     IHALWGADAS VVASDDGIVA RIPDTDGKLP DAAIFLFEPE KLLQIVREAV GSSALFAARF
     RECAARALLM PGRTPGHRTP LWQQRLRASQ LLEIAQGYPD FPVILETLRE CLQDVYDLPA
     LERLMRRLNG GEIQISDVTT TTPSPFATSL LFGYVAEFMY QSDAPLAERR ASVLSLDSEL
     LRNLLGQVDP GELLDPQVIR QVEEELQRLA PGRRAKGEEG LFDLLRELGP MTVEDLAQRH
     TGSSEEVASY LENLLAVKRI FPAMISGQER LACMDDAARL RDALGVRLPE SLPEIYLHRV
     SYPLRDLFLR YLRAHALVTA EQLAHEFSLG IAIVEEQLQQ LREQGLVMNL QQDIWVSDEV
     FRRLRLRSLQ AAREATRPVA ATTYARLLLE RQGVLPATDG SPALFASTSP GVYEGVDGVM
     RVIEQLAGVG LPASLWESQI LPARVRDYSS EMLDELLATG AVIWSGQKKL GEDDGLVALH
     LQEYAAESFT PAEADQANRS ALQQAIVAVL ADGGAWFAQQ ISQRIRDKIG ESVDLSALQE
     ALWALVWQGV ITSDIWAPLR ALTRSSSNAR TSTRRSHRAR RGRPVYAQPV SPRVSYNTPN
     LAGRWSLLQV EPLNDTERML ALAENMLDRY GIISRQAVIA ENIPGGFPSM QTLCRSMEDS
     GRIMRGRFVE GLGGAQFAER LTIDRLRDLA TQATQTRHYT PVALSANDPA NVWGNLLPWP
     AHPATLVPTR RAGALVVVSG GKLLLYLAQG GKKMLVWQEK EELLAPEVFH ALTTALRREP
     RLRFTLTEVN DLPVRQTPMF TLLREAGFSS SPQGLDWG
//
DBGET integrated database retrieval system