ID YIHN_ECOLI Reviewed; 421 AA.
AC P32135; Q2M8H1;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 27-MAR-2024, entry version 159.
DE RecName: Full=Inner membrane protein YihN {ECO:0000305};
GN Name=yihN; OrderedLocusNames=b3874, JW3845;
OS Escherichia coli (strain K12).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8346018; DOI=10.1093/nar/21.15.3391;
RA Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome. III. DNA sequence of the region
RT from 87.2 to 89.2 minutes.";
RL Nucleic Acids Res. 21:3391-3398(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS], AND SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [5]
RP INDUCTION.
RC STRAIN=K12 / BW25113;
RX PubMed=25346166; DOI=10.1080/09168451.2014.972328;
RA Yamamoto K., Nonaka G., Ozawa T., Takumi K., Ishihama A.;
RT "Induction of the Escherichia coli yijE gene expression by cystine.";
RL Biosci. Biotechnol. Biochem. 79:218-222(2015).
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- INDUCTION: Down-regulated by cystine. {ECO:0000269|PubMed:25346166}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; L19201; AAB03008.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76871.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77435.1; -; Genomic_DNA.
DR PIR; S40819; S40819.
DR RefSeq; NP_418310.1; NC_000913.3.
DR RefSeq; WP_000956313.1; NZ_SSZK01000026.1.
DR AlphaFoldDB; P32135; -.
DR SMR; P32135; -.
DR BioGRID; 4262634; 192.
DR DIP; DIP-12495N; -.
DR IntAct; P32135; 1.
DR STRING; 511145.b3874; -.
DR TCDB; 2.A.1.52.1; the major facilitator superfamily (mfs).
DR PaxDb; 511145-b3874; -.
DR EnsemblBacteria; AAC76871; AAC76871; b3874.
DR GeneID; 948365; -.
DR KEGG; ecj:JW3845; -.
DR KEGG; eco:b3874; -.
DR PATRIC; fig|1411691.4.peg.2837; -.
DR EchoBASE; EB1786; -.
DR eggNOG; COG2271; Bacteria.
DR HOGENOM; CLU_043790_0_0_6; -.
DR InParanoid; P32135; -.
DR OMA; TTIFLFW; -.
DR OrthoDB; 9773404at2; -.
DR PhylomeDB; P32135; -.
DR BioCyc; EcoCyc:YIHN-MONOMER; -.
DR PRO; PR:P32135; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR CDD; cd06174; MFS; 1.
DR Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR43826; GLUCOSE-6-PHOSPHATE EXCHANGER SLC37A4; 1.
DR PANTHER; PTHR43826:SF3; GLUCOSE-6-PHOSPHATE EXCHANGER SLC37A4; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; MFS general substrate transporter; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..421
FT /note="Inner membrane protein YihN"
FT /id="PRO_0000169676"
FT TOPO_DOM 1..44
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 66..73
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 74..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 92..95
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 96..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 119..146
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 168
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 190..220
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 263..288
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 310..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 331..354
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 376..385
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 386..406
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 407..421
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:15919996"
SQ SEQUENCE 421 AA; 46335 MW; 4FD9FAAA33A8E846 CRC64;
MLTKKKWALF SLLTLCGGTI YKLPSLKDAF YIPMQEYFHL TNGQIGNAMS VNSFVTTVGF
FLSIYFADKL PRRYTMSFSL IATGLLGVYL TTMPGYWGIL FVWALFGVTC DMMNWPVLLK
SVSRLGNSEQ QGRLFGFFET GRGIVDTVVA FSALAVFTWF GSGLLGFKAG IWFYSLIVIA
VGIIIFFVLN DKEEAPSVEV KKEDGASKNT SMTSVLKDKT IWLIAFNVFF VYAVYCGLTF
FIPFLKNIYL LPVALVGAYG IINQYCLKMI GGPIGGMISD KILKSPSKYL CYTFIISTAA
LVLLIMLPHE SMPVYLGMAC TLGFGAIVFT QRAVFFAPIG EAKIAENKTG AAMALGSFIG
YAPAMFCFSL YGYILDLNPG IIGYKIVFGI MACFAFSGAV VSVMLVKRIS QRKKEMLAAE
A
//
