ID NFXB_PSEAE Reviewed; 187 AA.
AC P32265;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 27-MAR-2024, entry version 141.
DE RecName: Full=HTH-type transcriptional regulator NfxB;
GN Name=nfxB; OrderedLocusNames=PA4600;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO;
RX PubMed=1330820; DOI=10.1016/0378-1097(92)90386-3;
RA Okazaki T., Hirai K.;
RT "Cloning and nucleotide sequence of the Pseudomonas aeruginosa nfxB gene,
RT conferring resistance to new quinolones.";
RL FEMS Microbiol. Lett. 76:197-202(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Confers resistance to guinolones. May negatively regulate the
CC expression of genes that are associated with cell permeability to
CC drugs.
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DR EMBL; X65646; CAA46598.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG07988.1; -; Genomic_DNA.
DR PIR; B83070; B83070.
DR PIR; S21466; S21466.
DR RefSeq; NP_253290.1; NC_002516.2.
DR RefSeq; WP_003094842.1; NZ_QZGE01000004.1.
DR AlphaFoldDB; P32265; -.
DR SMR; P32265; -.
DR STRING; 208964.PA4600; -.
DR PaxDb; 208964-PA4600; -.
DR DNASU; 881079; -.
DR GeneID; 881079; -.
DR KEGG; pae:PA4600; -.
DR PATRIC; fig|208964.12.peg.4815; -.
DR PseudoCAP; PA4600; -.
DR HOGENOM; CLU_069356_38_3_6; -.
DR InParanoid; P32265; -.
DR OrthoDB; 8654052at2; -.
DR PhylomeDB; P32265; -.
DR BioCyc; PAER208964:G1FZ6-4694-MONOMER; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0032993; C:protein-DNA complex; IPI:CollecTF.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0001217; F:DNA-binding transcription repressor activity; IPI:CollecTF.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:PseudoCAP.
DR GO; GO:0045892; P:negative regulation of DNA-templated transcription; IMP:PseudoCAP.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IBA:GO_Central.
DR Gene3D; 1.10.357.10; Tetracycline Repressor, domain 2; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR SUPFAM; SSF48498; Tetracyclin repressor-like, C-terminal domain; 1.
DR PROSITE; PS00356; HTH_LACI_1; 1.
PE 4: Predicted;
KW DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..187
FT /note="HTH-type transcriptional regulator NfxB"
FT /id="PRO_0000108013"
FT DNA_BIND 26..45
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT VARIANT 42
FT /note="R -> G (in mutant nfx13E; loss of DNA binding and
FT regulator activity)"
FT CONFLICT 124
FT /note="A -> E (in Ref. 1; CAA46598)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 187 AA; 21077 MW; FB8C6C1D14AFACAD CRC64;
MTLISHDERL IKALAVAIVD RPRATLKELA EAAGVSKATL HRFCGTRDNL VQMLEDHGET
VLNQIIQACD LEHAEPLEAL QRLIKEHLTH RELLVFLVFQ YRPDFLDPHG EGARWQSYLE
ALDAFFLRGQ QKGVFRIDIT AAVFTELFIT LVYGMVDAER RGRAASSNSA HTLEQMFLHG
ASNPARS
//
