ID RHA2_CAEEL Reviewed; 1148 AA.
AC P34305;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 06-JUN-2002, sequence version 2.
DT 27-MAR-2024, entry version 162.
DE RecName: Full=Putative ATP-dependent RNA helicase rha-2;
DE EC=3.6.4.13;
GN Name=rha-2; ORFNames=C06E1.10;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Probable ATP-binding RNA helicase.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC {ECO:0000305}.
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DR EMBL; FO080280; CCD62570.1; -; Genomic_DNA.
DR PIR; H88533; H88533.
DR RefSeq; NP_498895.1; NM_066494.1.
DR AlphaFoldDB; P34305; -.
DR SMR; P34305; -.
DR BioGRID; 41411; 6.
DR IntAct; P34305; 1.
DR STRING; 6239.C06E1.10.1; -.
DR iPTMnet; P34305; -.
DR EPD; P34305; -.
DR PaxDb; 6239-C06E1-10; -.
DR PeptideAtlas; P34305; -.
DR EnsemblMetazoa; C06E1.10.1; C06E1.10.1; WBGene00015525.
DR GeneID; 176207; -.
DR KEGG; cel:CELE_C06E1.10; -.
DR UCSC; C06E1.10; c. elegans.
DR AGR; WB:WBGene00015525; -.
DR WormBase; C06E1.10; CE29563; WBGene00015525; rha-2.
DR eggNOG; KOG0926; Eukaryota.
DR GeneTree; ENSGT00550000074985; -.
DR HOGENOM; CLU_001832_0_0_1; -.
DR InParanoid; P34305; -.
DR OMA; KYAYHCA; -.
DR OrthoDB; 5490433at2759; -.
DR PhylomeDB; P34305; -.
DR Reactome; R-CEL-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR PRO; PR:P34305; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00015525; Expressed in germ line (C elegans) and 4 other cell types or tissues.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0004386; F:helicase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0022414; P:reproductive process; IEA:UniProt.
DR CDD; cd17982; DEXHc_DHX37; 1.
DR CDD; cd18791; SF2_C_RHA; 1.
DR Gene3D; 1.20.120.1080; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR048333; HA2_WH.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR18934; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR18934:SF99; ATP-DEPENDENT RNA HELICASE DHX37-RELATED; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF21010; HA2_C; 1.
DR Pfam; PF04408; HA2_N; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW RNA-binding.
FT CHAIN 1..1148
FT /note="Putative ATP-dependent RNA helicase rha-2"
FT /id="PRO_0000055194"
FT DOMAIN 246..412
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 463..703
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 101..163
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 355..358
FT /note="DEAH box"
FT COMPBIAS 121..137
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 140..160
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 259..266
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 1148 AA; 129963 MW; 7BD99951D16D455A CRC64;
MGKRKTKEDN ASEDYDANEL MIVPGEKRKK LEKSGEKSAT GGKKNRNFAK EKEVAKLTKQ
AKRKLAAVQS RKALKQTQEE LFAGLAEFQL DPSKLCQLSS STKLSKEPEK APVFPEKLKV
FSGKTKTEAK RTQQDYYPTD DESSSEEEEE EEEGDNDIED AGNTVEVKIE PIDLDDVDEA
IDGNPETNLD QIVVKREDDE ESDNEDILAL PTTTVINRKK VIVERSKEIQ KSRAELPIFA
EEMRIVEAIN ENLVTVVCGE TGSGKTTQIP QFLYEAGYAS EGELIGITEP RRVAAIAMAQ
RVGVELAKPD EVSYQIRYEG TRSETTNILF MTDGVLMKEM EQDVMLKKYS VILIDEAHER
SMYSDVLIGM LSRIVPLRSK TARPLRLVIM SATLRLDDFT HKKLFPLLTP KVIKVDARQF
PVSVHFEKRT PDDYIASAFR KTCRIHETLP PGAILVFVTG QHEVKQLITK LKKRYPVVYE
TDKNGEVLVK GTKEWKEKKV EAAKSIKLED FKEETPETED FEDVDDGLMD GDDMNERGAA
EAFDDYEEFE NGDGDLSDGK VENSIGAPPA DCEPLYCLPL YSLLSMGKQR RVFDETPAGM
RLCVISTNVA ETSLTIPGVK YVIDGGFEKR RLYDSITGVS RFAVCRISQA SGDQRAGRAG
RISAGHAYRL YSSAVYQDFV KFADPEILSK PADQLVLHLK SMNIVKVVNF PFPSAPDEQM
LESAEKRLCR LGALSESTKN GKTEARITKL GKTLAVFPLA PSYAKFIAMA DQHNLMSHAI
LLISLLSVRE PLIPVSSLRG DTPEETKELM KNVLKERRRW CSHTGARRLG DLKVLMHAAS
VAEQIKYNAR ECEKVGLRVK ALVEARKLRQ QLTNIVNASC KKEHAAALDS DLPPPTDQQA
QLLRQMVVAS FSDRLARRVD RSVGQEEVQK GAYETTLIKG HVFIDPCSVV FTEEPEFVIY
QELVQVNEKK LMTSVCAVDK EWLSRLAESY CNYGEQDKNQ EPIYDPVKDM VVKTVKVTFG
PLNWELPNEN RSVPHDIMMY RYFALFLLDG LVFEKLKEYT PKLLAPPSTM VKSWAKLQKR
TEMLLNKLIE KEVTTRSSLK EQWLKNENWL LEEYLEWVPE SVHQQISLMW PPLEDHEKTI
KMGRNKKY
//
