ID EMB30_CAEEL Reviewed; 1027 AA.
AC P34441; Q9U4A4;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 23-MAY-2003, sequence version 4.
DT 27-MAR-2024, entry version 159.
DE RecName: Full=Abnormal embryogenesis protein 30;
GN Name=emb-30 {ECO:0000312|WormBase:F54C8.3};
GN ORFNames=F54C8.3 {ECO:0000312|WormBase:F54C8.3};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND MUTAGENESIS OF LEU-46; ALA-223;
RP GLY-224; GLU-360; GLU-483; LEU-564 AND GLY-701.
RC STRAIN=Bristol N2;
RX PubMed=10749938; DOI=10.1091/mbc.11.4.1401;
RA Furuta T., Tuck S., Kirchner J., Koch B., Auty R., Kitagawa R., Rose A.M.,
RA Greenstein D.;
RT "EMB-30: an APC4 homologue required for metaphase-to-anaphase transitions
RT during meiosis and mitosis in Caenorhabditis elegans.";
RL Mol. Biol. Cell 11:1401-1419(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP FUNCTION.
RX PubMed=11134076; DOI=10.1083/jcb.151.7.1469;
RA Golden A., Sadler P.L., Wallenfang M.R., Schumacher J.M., Hamill D.R.,
RA Bates G., Bowerman B., Seydoux G., Shakes D.C.;
RT "Metaphase to anaphase (mat) transition-defective mutants in Caenorhabditis
RT elegans.";
RL J. Cell Biol. 151:1469-1482(2000).
RN [5]
RP FUNCTION.
RX PubMed=12498686; DOI=10.1016/s0960-9822(02)01392-1;
RA Kitagawa R., Law E., Tang L., Rose A.M.;
RT "The Cdc20 homolog, FZY-1, and its interacting protein, IFY-1, are required
RT for proper chromosome segregation in Caenorhabditis elegans.";
RL Curr. Biol. 12:2118-2123(2002).
RN [6]
RP FUNCTION.
RX PubMed=11832245; DOI=10.1016/s1534-5807(02)00114-4;
RA Rappleye C.A., Tagawa A., Lyczak R., Bowerman B., Aroian R.V.;
RT "The anaphase-promoting complex and separin are required for embryonic
RT anterior-posterior axis formation.";
RL Dev. Cell 2:195-206(2002).
RN [7]
RP FUNCTION.
RX PubMed=15556870; DOI=10.1016/j.cub.2004.11.010;
RA Juo P., Kaplan J.M.;
RT "The anaphase-promoting complex regulates the abundance of GLR-1 glutamate
RT receptors in the ventral nerve cord of C. elegans.";
RL Curr. Biol. 14:2057-2062(2004).
RN [8]
RP FUNCTION, AND MUTAGENESIS OF THR-265 AND MET-480.
RX PubMed=17237515; DOI=10.1534/genetics.106.067918;
RA Tarailo M., Kitagawa R., Rose A.M.;
RT "Suppressors of spindle checkpoint defect (such) mutants identify new mdf-
RT 1/MAD1 interactors in Caenorhabditis elegans.";
RL Genetics 175:1665-1679(2007).
RN [9]
RP FUNCTION.
RX PubMed=24321454; DOI=10.1016/j.mcn.2013.12.001;
RA Kowalski J.R., Dube H., Touroutine D., Rush K.M., Goodwin P.R., Carozza M.,
RA Didier Z., Francis M.M., Juo P.;
RT "The anaphase-promoting complex (APC) ubiquitin ligase regulates GABA
RT transmission at the C. elegans neuromuscular junction.";
RL Mol. Cell. Neurosci. 58:62-75(2014).
CC -!- FUNCTION: Probable component of the anaphase promoting
CC complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase
CC that controls progression through mitosis and the G1 phase of the cell
CC cycle (By similarity). The APC/C complex acts by mediating
CC ubiquitination and subsequent degradation of target proteins (By
CC similarity). Developmental role in early embryogenesis and the
CC metaphase to anaphase transition in oocyte and spermatocyte meiosis and
CC mitosis in somatic and germ cells (PubMed:10749938, PubMed:12498686,
CC PubMed:11134076, PubMed:17237515). Required for embryonic anterior-
CC posterior axis formation (PubMed:11832245). Negatively regulates ify-1
CC protein levels during meiosis I (PubMed:12498686). Plays a role in
CC regulating the abundance of glr-1 receptors in postmitotic neurons,
CC which may in turn control animal locomotion (PubMed:15556870). Involved
CC in regulating GABA neurotransmitter release at neuromuscular junctions
CC in GABA motor neurons (PubMed:24321454). {ECO:0000250|UniProtKB:Q9UJX5,
CC ECO:0000269|PubMed:10749938, ECO:0000269|PubMed:11134076,
CC ECO:0000269|PubMed:11832245, ECO:0000269|PubMed:12498686,
CC ECO:0000269|PubMed:15556870, ECO:0000269|PubMed:17237515,
CC ECO:0000269|PubMed:24321454}.
CC -!- PATHWAY: Protein modification; protein ubiquitination. {ECO:0000305}.
CC -!- SUBUNIT: The APC/C is probably composed of at least 12 subunits: apc-2,
CC apc-10, apc-11, cdc-26, emb-1, emb-27, emb-30, mat-1, mat-2, mat-3,
CC such-1 and gfi-3. {ECO:0000305}.
CC -!- INTERACTION:
CC P34441; P34766: pal-1; NbExp=2; IntAct=EBI-311903, EBI-311911;
CC -!- DOMAIN: The C-terminus is specifically required for completion of
CC oocyte meiotic divisions.
CC -!- SIMILARITY: Belongs to the APC4 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF192400; AAF24233.1; -; mRNA.
DR EMBL; Z22178; CAA80159.3; -; Genomic_DNA.
DR PIR; G88554; G88554.
DR PIR; S40745; S40745.
DR RefSeq; NP_499074.2; NM_066673.6.
DR AlphaFoldDB; P34441; -.
DR SMR; P34441; -.
DR BioGRID; 41519; 17.
DR ComplexPortal; CPX-3382; Anaphase-promoting complex.
DR IntAct; P34441; 3.
DR STRING; 6239.F54C8.3.2; -.
DR EPD; P34441; -.
DR PaxDb; 6239-F54C8-3; -.
DR PeptideAtlas; P34441; -.
DR EnsemblMetazoa; F54C8.3.1; F54C8.3.1; WBGene00001284.
DR GeneID; 176322; -.
DR KEGG; cel:CELE_F54C8.3; -.
DR UCSC; F54C8.3; c. elegans.
DR AGR; WB:WBGene00001284; -.
DR WormBase; F54C8.3; CE32676; WBGene00001284; emb-30.
DR eggNOG; ENOG502SDZW; Eukaryota.
DR HOGENOM; CLU_004796_0_0_1; -.
DR InParanoid; P34441; -.
DR OMA; MMQELGH; -.
DR OrthoDB; 391429at2759; -.
DR Reactome; R-CEL-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR SignaLink; P34441; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:P34441; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00001284; Expressed in germ line (C elegans) and 4 other cell types or tissues.
DR GO; GO:0005680; C:anaphase-promoting complex; IBA:GO_Central.
DR GO; GO:0034399; C:nuclear periphery; IBA:GO_Central.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR GO; GO:0008595; P:anterior/posterior axis specification, embryo; IMP:WormBase.
DR GO; GO:0008356; P:asymmetric cell division; IMP:UniProtKB.
DR GO; GO:1990949; P:metaphase/anaphase transition of meiosis I; IMP:UniProtKB.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR GO; GO:0009949; P:polarity specification of anterior/posterior axis; IMP:UniProtKB.
DR GO; GO:0070979; P:protein K11-linked ubiquitination; IBA:GO_Central.
DR GO; GO:0051445; P:regulation of meiotic cell cycle; NAS:ComplexPortal.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; NAS:ComplexPortal.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR024789; APC4.
DR InterPro; IPR024977; Apc4-like_WD40_dom.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR13260; ANAPHASE PROMOTING COMPLEX SUBUNIT 4 APC4; 1.
DR PANTHER; PTHR13260:SF0; ANAPHASE-PROMOTING COMPLEX SUBUNIT 4; 1.
DR Pfam; PF12894; ANAPC4_WD40; 1.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Developmental protein; Meiosis; Mitosis;
KW Neurotransmitter transport; Reference proteome; Repeat; Transport;
KW Ubl conjugation pathway; WD repeat.
FT CHAIN 1..1027
FT /note="Abnormal embryogenesis protein 30"
FT /evidence="ECO:0000305"
FT /id="PRO_0000050959"
FT REPEAT 18..65
FT /note="WD 1"
FT REPEAT 70..109
FT /note="WD 2"
FT REGION 619..655
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1005..1027
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 622..649
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 46
FT /note="L->W: In TN481."
FT /evidence="ECO:0000269|PubMed:10749938"
FT MUTAGEN 223
FT /note="A->V: In TN478."
FT /evidence="ECO:0000269|PubMed:10749938"
FT MUTAGEN 224
FT /note="G->D: In TN479."
FT /evidence="ECO:0000269|PubMed:10749938"
FT MUTAGEN 265
FT /note="T->I: In h1962ts; temperature sensitive mutant that
FT can be maintained at 20 degrees Celsius, but that results
FT in the production of unfertilized eggs and therefore
FT sterility at 25 degrees Celsius."
FT /evidence="ECO:0000269|PubMed:17237515"
FT MUTAGEN 360
FT /note="E->K: In G53."
FT /evidence="ECO:0000269|PubMed:10749938"
FT MUTAGEN 480
FT /note="M->I: In h1959ts; temperature sensitive mutant that
FT can be maintained at 20 degrees Celsius, but that results
FT in embryonic lethality at 25 degrees Celsius."
FT /evidence="ECO:0000269|PubMed:17237515"
FT MUTAGEN 483
FT /note="E->K: In AX69."
FT /evidence="ECO:0000269|PubMed:10749938"
FT MUTAGEN 564
FT /note="L->P: In TN494."
FT /evidence="ECO:0000269|PubMed:10749938"
FT MUTAGEN 701
FT /note="G->R: In TN377."
FT /evidence="ECO:0000269|PubMed:10749938"
SQ SEQUENCE 1027 AA; 117051 MW; A3D35E7463303888 CRC64;
MDFSIVKTQI LNNRRTFRTP FKVSSMCFSS QNDLIALGSK TGEILLKRTS WKMIWKTNIN
MIQAVGTECK LDSSVSALHF SPDGRFLAAA TSKGIIHLLD VETGKVRFSV KAASEKIAKL
HWNCVREKPF ISNLGEFTTR IKNVEAIEGA IELAETTPNI SQEEIAFVYQ RLDEDGSSFK
HEDAHKESLE RTLISTETFR ESLQNTILLA TDDMDSKIIV LVAGVFPYME IDISDTLLQY
NQSLLMLYDM HYSSAFGGVS FLATTYGPFL DCKQNELKPP GAEPKKDGQG CHTLLFNVKL
NINSSLWDTA LRYIRLLFGF NLYSISLETT KRNWEEQIDN LHSLFDTKTK AVKIGNVLLE
MLLSGSTDAA GEAFLERGLG TDGLDKIELF ATKHMPEVCR IARGQLSTSA RNLCFQRCEF
STSLSRYAKF IQLKDDDSFL YDEDPPAYLS ESNIWLNTLE EKINILDMKT RHLGIQCLTM
MQELGHLVKW ISMTKPFAKT MKVNALMKIK RMNIAKILLY IVRNFIPDPE AVKDIENRLF
NLKELVSKQK KKDLEDKFDE VEYLYRLERI AEVERKKLIE TFHEKFRFAD LDDDDLTPLL
FQELDHPIDI FTDSMMEQND SSPFLEEDEG EPEQEEQKPD EEPEPEGEPQ PACDLDRVGS
FFEKELSQKA LEVLPKCDET FDCVLNERGS RMNSIEIQKM GILRVLEDLA ATLSSPQKIH
CDKAGNQKKL EVSFIYEITS TPTHQGYQDA KISSVTPSYY KRHPFNQLSG MGRSIQVSVL
QKNEFSSIVI VPTSDVLPED ENEVNDHVEK LKRCYEFEKI DVTVDKSSAE NRPPGETDAM
EVDSNELRID VDLGPVVPEY DTLIELSQVH PLHHGELVLL GKFTSAQEGT GVMSQMMRSI
SSYPHEIPED KDVFAKNSSE DGSSEIPIET LIIHPTIQLA AYLHDDGSKI TIGDMKPEVP
PIADYEEKTV RKTFRDYQRR RERYREDMGV MNMNDVQYDV LVQEAMDSGR DLTDNGESDE
DEEDDDI
//
