GenomeNet

Database: UniProt
Entry: P35596
LinkDB: P35596
Original site: P35596 
ID   GLPO_STRPN              Reviewed;         608 AA.
AC   P35596; O87017;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   26-SEP-2001, sequence version 2.
DT   10-APR-2019, entry version 126.
DE   RecName: Full=Alpha-glycerophosphate oxidase;
DE            EC=1.1.3.21;
DE   AltName: Full=Exported protein 6;
DE   AltName: Full=Glycerol-3-phosphate oxidase;
GN   Name=glpO; Synonyms=exp6; OrderedLocusNames=SP_2185;
OS   Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=170187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 27336;
RX   PubMed=9726992; DOI=10.1074/jbc.273.37.23812;
RA   Parsonage D., Luba J., Mallett T.C., Claiborne A.;
RT   "The soluble alpha-glycerophosphate oxidase from Enterococcus
RT   casseliflavus. Sequence homology with the membrane-associated
RT   dehydrogenase and kinetic analysis of the recombinant enzyme.";
RL   J. Biol. Chem. 273:23812-23822(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-334 / TIGR4;
RX   PubMed=11463916; DOI=10.1126/science.1061217;
RA   Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA   Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA   Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA   Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA   Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA   McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T.,
RA   Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C.,
RA   Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT   "Complete genome sequence of a virulent isolate of Streptococcus
RT   pneumoniae.";
RL   Science 293:498-506(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 271-408.
RC   STRAIN=R6x;
RX   PubMed=7934910; DOI=10.1111/j.1365-2958.1993.tb01233.x;
RA   Pearce B.J., Yin Y.B., Masure H.R.;
RT   "Genetic identification of exported proteins in Streptococcus
RT   pneumoniae.";
RL   Mol. Microbiol. 9:1037-1050(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=O2 + sn-glycerol 3-phosphate = dihydroxyacetone phosphate
CC         + H2O2; Xref=Rhea:RHEA:18369, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16240, ChEBI:CHEBI:57597, ChEBI:CHEBI:57642;
CC         EC=1.1.3.21;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC   -!- PATHWAY: Membrane lipid metabolism; glycerophospholipid
CC       metabolism.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC   -!- SIMILARITY: Belongs to the FAD-dependent glycerol-3-phosphate
CC       dehydrogenase family. {ECO:0000305}.
DR   EMBL; U94770; AAC34740.1; -; Genomic_DNA.
DR   EMBL; AE005672; AAK76236.1; -; Genomic_DNA.
DR   EMBL; L20560; AAA26882.1; -; Genomic_DNA.
DR   PIR; C95255; C95255.
DR   RefSeq; WP_000395957.1; NZ_AKVY01000001.1.
DR   ProteinModelPortal; P35596; -.
DR   SMR; P35596; -.
DR   STRING; 170187.SP_2185; -.
DR   EnsemblBacteria; AAK76236; AAK76236; SP_2185.
DR   KEGG; spn:SP_2185; -.
DR   eggNOG; ENOG4105C6V; Bacteria.
DR   eggNOG; COG0578; LUCA.
DR   HOGENOM; HOG000004812; -.
DR   KO; K00105; -.
DR   OMA; MDNPTVK; -.
DR   BioCyc; SPNE170187:G1FZB-2283-MONOMER; -.
DR   UniPathway; UPA00940; -.
DR   Proteomes; UP000000585; Chromosome.
DR   GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004368; F:glycerol-3-phosphate dehydrogenase (quinone) activity; IEA:InterPro.
DR   GO; GO:0004369; F:glycerol-3-phosphate oxidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006072; P:glycerol-3-phosphate metabolic process; IEA:InterPro.
DR   GO; GO:0006650; P:glycerophospholipid metabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.8.870; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   InterPro; IPR031656; DAO_C.
DR   InterPro; IPR038299; DAO_C_sf.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR000447; G3P_DH_FAD-dep.
DR   PANTHER; PTHR11985; PTHR11985; 1.
DR   Pfam; PF01266; DAO; 1.
DR   Pfam; PF16901; DAO_C; 1.
DR   PRINTS; PR01001; FADG3PDH.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   PROSITE; PS00977; FAD_G3PDH_1; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Complete proteome; FAD; Flavoprotein;
KW   Glycerol metabolism; Membrane; Oxidoreductase.
FT   CHAIN         1    608       Alpha-glycerophosphate oxidase.
FT                                /FTId=PRO_0000126108.
FT   NP_BIND      21     49       FAD. {ECO:0000255}.
FT   CONFLICT    100    100       S -> P (in Ref. 1; AAC34740).
FT                                {ECO:0000305}.
FT   CONFLICT    138    138       A -> T (in Ref. 1; AAC34740).
FT                                {ECO:0000305}.
FT   CONFLICT    275    275       Y -> S (in Ref. 3; AAA26882).
FT                                {ECO:0000305}.
FT   CONFLICT    367    367       S -> R (in Ref. 3; AAA26882).
FT                                {ECO:0000305}.
FT   CONFLICT    544    544       T -> A (in Ref. 1; AAC34740).
FT                                {ECO:0000305}.
FT   CONFLICT    572    572       I -> V (in Ref. 1; AAC34740).
FT                                {ECO:0000305}.
SQ   SEQUENCE   608 AA;  66796 MW;  A29B42FF4A75E406 CRC64;
     MEFSKKTREL SIKKMQERTL DLLIIGGGIT GAGVALQAAA SGLETGLIEM QDFAEGTSSR
     STKLVHGGLR YLKQFDVEVV SDTVSERAVV QQIAPHIPKS DPMLLPVYDE DGATFSLFRL
     KVAMDLYDLL AGVSNTPAAN KVLSKDQVLE RQPNLKKEGL VGGGVYLDFR NNDARLVIEN
     IKRANQDGAL IANHVKAEGF LFDESGKITG VVARDLLTDQ VFEIKARLVI NTTGPWSDKV
     RNLSNKGTQF SQMRPTKGVH LVVDSSKIKV SQPVYFDTGL GDGRMVFVLP RENKTYFGTT
     DTDYTGDLEH PKVTQEDVDY LLGIVNNRFP ESNITIDDIE SSWAGLRPLI AGNSASDYNG
     GNNGTISDES FDNLIATVES YLSKEKTRED VESAVSKLES STSEKHLDPS AVSRGSSLDR
     DDNGLLTLAG GKITDYRKMA EGAMERVVDI LKAEFDRSFK LINSKTYPVS GGELNPANVD
     SEIEAFAQLG VSRGLDSKEA HYLANLYGSN APKVFALAHS LEQAPGLSLA DTLSLHYAMR
     NELTLSPVDF LLRRTNHMLF MRDSLDSIVE PILDEMGRFY DWTEEEKATY RADVEAALAN
     NDLAELKN
//
DBGET integrated database retrieval system