ID DHON_METGL Reviewed; 412 AA.
AC P37144;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-MAY-2023, entry version 90.
DE RecName: Full=Homoserine dehydrogenase;
DE Short=HDH;
DE EC=1.1.1.3;
GN Name=hom;
OS Methylobacillus glycogenes.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
OC Methylophilaceae; Methylobacillus.
OX NCBI_TaxID=406;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 21371 / DSM 1760 / JCM 2853 / NCIMB 1210 / M 135-7;
RX PubMed=8117070; DOI=10.1128/aem.60.1.111-119.1994;
RA Motoyama H., Maki K., Anazawa H., Ishino S., Teshiba S.;
RT "Cloning and nucleotide sequences of the homoserine dehydrogenase genes
RT (hom) and the threonine synthase genes (thrC) of the Gram-negative obligate
RT methylotroph Methylobacillus glycogenes.";
RL Appl. Environ. Microbiol. 60:111-119(1994).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-homoserine + NADP(+) = H(+) + L-aspartate 4-semialdehyde +
CC NADPH; Xref=Rhea:RHEA:15761, ChEBI:CHEBI:15378, ChEBI:CHEBI:57476,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:537519; EC=1.1.1.3;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-homoserine + NAD(+) = H(+) + L-aspartate 4-semialdehyde +
CC NADH; Xref=Rhea:RHEA:15757, ChEBI:CHEBI:15378, ChEBI:CHEBI:57476,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:537519; EC=1.1.1.3;
CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC pathway; L-homoserine from L-aspartate: step 3/3.
CC -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine
CC from L-aspartate: step 3/5.
CC -!- SIMILARITY: Belongs to the homoserine dehydrogenase family.
CC {ECO:0000305}.
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DR EMBL; D14071; BAA40415.1; -; Genomic_DNA.
DR AlphaFoldDB; P37144; -.
DR SMR; P37144; -.
DR UniPathway; UPA00050; UER00063.
DR UniPathway; UPA00051; UER00465.
DR GO; GO:0004412; F:homoserine dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009088; P:threonine biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd04881; ACT_HSDH-Hom; 1.
DR Gene3D; 3.30.70.260; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR002912; ACT_dom.
DR InterPro; IPR005106; Asp/hSer_DH_NAD-bd.
DR InterPro; IPR016204; HDH.
DR InterPro; IPR001342; HDH_cat.
DR InterPro; IPR019811; HDH_CS.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR43331; HOMOSERINE DEHYDROGENASE; 1.
DR PANTHER; PTHR43331:SF1; HOMOSERINE DEHYDROGENASE; 1.
DR Pfam; PF01842; ACT; 1.
DR Pfam; PF00742; Homoserine_dh; 1.
DR Pfam; PF03447; NAD_binding_3; 1.
DR PIRSF; PIRSF000098; Homoser_dehydrog; 1.
DR SUPFAM; SSF55021; ACT-like; 1.
DR SUPFAM; SSF55347; Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS51671; ACT; 1.
DR PROSITE; PS01042; HOMOSER_DHGENASE; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW Isoleucine biosynthesis; Methionine biosynthesis; NADP; Oxidoreductase;
KW Threonine biosynthesis.
FT CHAIN 1..412
FT /note="Homoserine dehydrogenase"
FT /id="PRO_0000066699"
FT DOMAIN 330..407
FT /note="ACT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01007"
FT ACT_SITE 205
FT /note="Proton donor"
FT /evidence="ECO:0000255"
FT BINDING 9..16
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 105
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 190
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 412 AA; 44818 MW; 613A1B7FDECEF4AA CRC64;
MKPINVGLLG IGTVGGGTYT VLTRNQEEIA RRAGRPIAIT RVADRNLELA RQVTGGKIDV
TDDAFAIVSD PAIDIVVELI GGYTVARELV LKAIENGKHV VTANKALIAC MAMKFLPLRR
KKASSSLLKL PLLVVSPLFK AVREGLAANR IEWIAGIING TTNFILSEMR EKGLAFADVL
KEAQRLGYAE ADPTFDVEGI DAAHKLMILA AMLWLFVHSL CRGITKLDAV DITKRTDKGV
ELRVHPTLIP EKRLICQCEW RNECCAGQGR CCWPTLYYGA GAGAEPTASA VADLVDGTDR
GISCPHLAFQ PDRLVDLPIL PIGEISSAYY LRLRAVDKPG VLADVTRILG DRQISIDAMI
QKEPQEGEDQ ADIIILTHVT VEKNMDDAIA AIEALPAISG KVTRLRMEEL SR
//
