ID YHK8_YEAST Reviewed; 514 AA.
AC P38776; D3DKZ6;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 27-MAR-2024, entry version 144.
DE RecName: Full=Probable drug/proton antiporter YHK8;
GN Name=YHK8; OrderedLocusNames=YHR048W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP INDUCTION.
RX PubMed=12697649; DOI=10.1093/jac/dkg217;
RA Barker K.S., Pearson M.M., Rogers P.D.;
RT "Identification of genes differentially expressed in association with
RT reduced azole susceptibility in Saccharomyces cerevisiae.";
RL J. Antimicrob. Chemother. 51:1131-1140(2003).
RN [4]
RP PREDICTION OF FUNCTION.
RX PubMed=16630275; DOI=10.1111/j.1567-1364.2006.00058.x;
RA Gbelska Y., Krijger J.J., Breunig K.D.;
RT "Evolution of gene families: the multidrug resistance transporter genes in
RT five related yeast species.";
RL FEMS Yeast Res. 6:345-355(2006).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Probable drug/proton antiporter.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- INDUCTION: Up-regulated in cells exhibiting reduced susceptibility to
CC azoles. {ECO:0000269|PubMed:12697649}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. CAR1 family.
CC {ECO:0000305}.
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DR EMBL; U00062; AAB68902.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06740.1; -; Genomic_DNA.
DR PIR; S46733; S46733.
DR RefSeq; NP_011914.1; NM_001179178.1.
DR AlphaFoldDB; P38776; -.
DR BioGRID; 36480; 87.
DR DIP; DIP-8197N; -.
DR IntAct; P38776; 1.
DR MINT; P38776; -.
DR STRING; 4932.YHR048W; -.
DR TCDB; 2.A.1.2.63; the major facilitator superfamily (mfs).
DR PaxDb; 4932-YHR048W; -.
DR PeptideAtlas; P38776; -.
DR EnsemblFungi; YHR048W_mRNA; YHR048W; YHR048W.
DR GeneID; 856444; -.
DR KEGG; sce:YHR048W; -.
DR AGR; SGD:S000001090; -.
DR SGD; S000001090; YHK8.
DR VEuPathDB; FungiDB:YHR048W; -.
DR eggNOG; KOG0255; Eukaryota.
DR HOGENOM; CLU_008455_11_1_1; -.
DR InParanoid; P38776; -.
DR OMA; DPYEVGW; -.
DR OrthoDB; 2032876at2759; -.
DR BioCyc; YEAST:G3O-31103-MONOMER; -.
DR BioGRID-ORCS; 856444; 5 hits in 10 CRISPR screens.
DR PRO; PR:P38776; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38776; Protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005886; C:plasma membrane; ISS:SGD.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:1902600; P:proton transmembrane transport; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR GO; GO:1990961; P:xenobiotic detoxification by transmembrane export across the plasma membrane; ISA:SGD.
DR CDD; cd17323; MFS_Tpo1_MDR_like; 1.
DR Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR PANTHER; PTHR23502:SF7; DRUG_PROTON ANTIPORTER YHK8-RELATED; 1.
DR PANTHER; PTHR23502; MAJOR FACILITATOR SUPERFAMILY; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; MFS general substrate transporter; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Antiport; Hydrogen ion transport; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..514
FT /note="Probable drug/proton antiporter YHK8"
FT /id="PRO_0000173440"
FT TOPO_DOM 1..74
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 96..111
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 133..141
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 163..170
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 192..200
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 222..227
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 249..307
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 329..342
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 364..386
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 408..412
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 434..447
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 448..468
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 469..477
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 478..498
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 499..514
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 514 AA; 57838 MW; 2CAAD39047F1BBCB CRC64;
MVAEFQIASA QSSALTSTEE EHCSINSDKA AKLDLELTSE RKNDGKQSHE VTFNEDIADP
EDIARHMSTA RRYYISSLIT FTSMVITMIS SSWTLPSTHI IEHFHISHEV STLGITLYVF
GLGIGPLFLS PLSELYGRRI TFLYALTLSI IWQCLTIWSK TITGVMFGRF LSGFFGSAFL
SVAGGAIADI FDKDQIGIPM AIYTTSAFLG PSLGPIIGGA LYHQSYKWTF ITLLITSGCC
LVMIIFTIPE TYKPMLLIRK AKRLRKEKND QRYYAVLEVT REQTSLLSAI FLSTKRPFGL
LLRDRMMGVL CFYTGLELAI IYLYFVAFPY VFKKLYNFGP MEIACSYIGI MVGMILSAPT
CLLFQKTFEW RVKRNNGVKT PEMRFEPLFY GAFLTPVGLF IFAFTCYKHV HWIAPIIGSA
IFGSGVYFVF TGVFAYTVDA YRRYAASGMA CNTFVRCIMA GVFPLFGLQM YKSMGVNWAG
FLLAMVTVAM IPVPFLFTKY GARLRAKSPY AWDD
//
