ID BGLJ_ECOLI Reviewed; 225 AA.
AC P39404; Q2M5V0;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 3.
DT 27-MAR-2024, entry version 165.
DE RecName: Full=Transcriptional activator protein BglJ;
GN Name=bglJ; Synonyms=yjjR; OrderedLocusNames=b4366, JW5955;
OS Escherichia coli (strain K12).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ROLE IN BGL OPERON EXPRESSION.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=8725214; DOI=10.1093/genetics/143.2.627;
RA Giel M., Desnoyer M., Lopilato J.;
RT "A mutation in a new gene, bglJ, activates the bgl operon in Escherichia
RT coli K-12.";
RL Genetics 143:627-635(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP OPERON STRUCTURE, AND INDUCTION.
RC STRAIN=CSH50;
RX PubMed=18055596; DOI=10.1128/jb.01447-07;
RA Stratmann T., Madhusudan S., Schnetz K.;
RT "Regulation of the yjjQ-bglJ operon, encoding LuxR-type transcription
RT factors, and the divergent yjjP gene by H-NS and LeuO.";
RL J. Bacteriol. 190:926-935(2008).
RN [6]
RP OPERON STRUCTURE, AND INDUCTION.
RC STRAIN=K12 / BW25113;
RX PubMed=19429622; DOI=10.1128/jb.00108-09;
RA Shimada T., Yamamoto K., Ishihama A.;
RT "Involvement of the leucine response transcription factor LeuO in
RT regulation of the genes for sulfa drug efflux.";
RL J. Bacteriol. 191:4562-4571(2009).
RN [7]
RP PROTEIN START SITE, AND INTERACTION WITH RCSB.
RC STRAIN=CSH50;
RX PubMed=20952573; DOI=10.1128/jb.00807-10;
RA Venkatesh G.R., Kembou Koungni F.C., Paukner A., Stratmann T.,
RA Blissenbach B., Schnetz K.;
RT "BglJ-RcsB heterodimers relieve repression of the Escherichia coli bgl
RT operon by H-NS.";
RL J. Bacteriol. 192:6456-6464(2010).
CC -!- FUNCTION: A crytic transcriptional activator. When its expression is
CC induced it relieves H-NS repression of the bgl operon. Acts
CC independently of transcription factor LeuO.
CC {ECO:0000269|PubMed:8725214}.
CC -!- SUBUNIT: Forms a complex with RcsB; genetically both BglJ and RcsB are
CC required to relieve bgl operon repression by H-NS and by StpA.
CC -!- INTERACTION:
CC P39404; P0DMC7: rcsB; NbExp=4; IntAct=EBI-551429, EBI-369670;
CC -!- INDUCTION: Repressed by H-NS, activated by LeuO. Member of the yjjQ-
CC bglJ operon. {ECO:0000269|PubMed:18055596,
CC ECO:0000269|PubMed:19429622}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE78356.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U35834; AAB49332.1; -; Genomic_DNA.
DR EMBL; U14003; AAA97265.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77322.4; -; Genomic_DNA.
DR EMBL; AP009048; BAE78356.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_418786.4; NC_000913.3.
DR RefSeq; WP_001301727.1; NZ_STEB01000025.1.
DR AlphaFoldDB; P39404; -.
DR SMR; P39404; -.
DR BioGRID; 4262783; 186.
DR ComplexPortal; CPX-5763; bglJ-rcsB DNA-binding transcription factor complex.
DR DIP; DIP-9217N; -.
DR IntAct; P39404; 2.
DR STRING; 511145.b4366; -.
DR PaxDb; 511145-b4366; -.
DR EnsemblBacteria; AAC77322; AAC77322; b4366.
DR GeneID; 948889; -.
DR KEGG; ecj:JW5955; -.
DR KEGG; eco:b4366; -.
DR PATRIC; fig|1411691.4.peg.2320; -.
DR EchoBASE; EB2479; -.
DR eggNOG; COG2197; Bacteria.
DR HOGENOM; CLU_000445_90_6_6; -.
DR InParanoid; P39404; -.
DR OMA; FNAMTKL; -.
DR OrthoDB; 6623825at2; -.
DR PhylomeDB; P39404; -.
DR BioCyc; EcoCyc:G7948-MONOMER; -.
DR PRO; PR:P39404; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005667; C:transcription regulator complex; IPI:ComplexPortal.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; IDA:ComplexPortal.
DR GO; GO:0043470; P:regulation of carbohydrate catabolic process; IDA:ComplexPortal.
DR GO; GO:0009314; P:response to radiation; IMP:EcoCyc.
DR CDD; cd06170; LuxR_C_like; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR43214:SF31; TRANSCRIPTIONAL ACTIVATOR PROTEIN BGLJ; 1.
DR PANTHER; PTHR43214; TWO-COMPONENT RESPONSE REGULATOR; 1.
DR Pfam; PF00196; GerE; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SUPFAM; SSF46894; C-terminal effector domain of the bipartite response regulators; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..225
FT /note="Transcriptional activator protein BglJ"
FT /id="PRO_0000184140"
FT DOMAIN 146..211
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 170..189
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
SQ SEQUENCE 225 AA; 25622 MW; 5EED6E444409A010 CRC64;
MEHSRIKKRN VALIEKCVMS SIGIESLFRK FAGNPYKLHT YTSQESFQDA MSRISFAAVI
FSFSAMRSER REGLSCLTEL AIKFPRTRRL VIADDDIEAR LIGSLSPSPL DGVLSKASTL
EIFHQELFLS LNGVRQATDR LNNQWYINQS RTLSPTEREI LRFMSRGYSM TQIAEQLKRN
IKTIRAHKFN VMSKLGVSSD AGLLEAADIL LCMRHCETSN VLHPY
//
