ID NIT1_YEAST Reviewed; 199 AA.
AC P40447; D6VVC3; Q05729; Q6Q5A1;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 24-JAN-2024, entry version 156.
DE RecName: Full=Putative nitrilase-like protein NIT1;
GN Name=NIT1; OrderedLocusNames=YIL164C; ORFNames=YI9402.10BC;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Doignon F., Pilatte E., Aigle M.;
RT "Study of a DNA fragment involved in the ergosterol biosynthesis.";
RL Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
CC -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC Nitrilase family. {ECO:0000305}.
CC -!- CAUTION: Could be the product of a pseudogene. NIT1/YIL164C seems to be
CC the N-terminal part of a putative nitrilase-like protein formed of
CC NIT1/YIL164C and YIL165C. {ECO:0000305}.
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DR EMBL; X66132; CAA46923.1; -; mRNA.
DR EMBL; Z46921; CAA87028.1; -; Genomic_DNA.
DR EMBL; AY558236; AAS56562.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08389.1; -; Genomic_DNA.
DR PIR; S50363; S50363.
DR RefSeq; NP_012102.1; NM_001179512.1.
DR AlphaFoldDB; P40447; -.
DR SMR; P40447; -.
DR BioGRID; 34829; 93.
DR STRING; 4932.YIL164C; -.
DR PaxDb; 4932-YIL164C; -.
DR PeptideAtlas; P40447; -.
DR EnsemblFungi; YIL164C_mRNA; YIL164C; YIL164C.
DR GeneID; 854642; -.
DR KEGG; sce:YIL164C; -.
DR AGR; SGD:S000001426; -.
DR SGD; S000001426; NIT1.
DR VEuPathDB; FungiDB:YIL164C; -.
DR eggNOG; KOG0805; Eukaryota.
DR GeneTree; ENSGT00530000066464; -.
DR HOGENOM; CLU_030130_6_2_1; -.
DR InParanoid; P40447; -.
DR OMA; MNSERTH; -.
DR OrthoDB; 2785533at2759; -.
DR BioCyc; YEAST:YIL164C-MONOMER; -.
DR BioGRID-ORCS; 854642; 4 hits in 10 CRISPR screens.
DR ChiTaRS; NIT1; yeast.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P40447; Protein.
DR GO; GO:0000257; F:nitrilase activity; ISA:SGD.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR Gene3D; 3.60.110.10; Carbon-nitrogen hydrolase; 1.
DR InterPro; IPR003010; C-N_Hydrolase.
DR InterPro; IPR036526; C-N_Hydrolase_sf.
DR InterPro; IPR000132; Nitrilase/CN_hydratase_CS.
DR InterPro; IPR044149; Nitrilases_CHs.
DR PANTHER; PTHR46044:SF1; CN HYDROLASE DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR46044; NITRILASE; 1.
DR Pfam; PF00795; CN_hydrolase; 1.
DR SUPFAM; SSF56317; Carbon-nitrogen hydrolase; 1.
DR PROSITE; PS50263; CN_HYDROLASE; 1.
DR PROSITE; PS00920; NITRIL_CHT_1; 1.
DR PROSITE; PS00921; NITRIL_CHT_2; 1.
PE 5: Uncertain;
KW Reference proteome.
FT CHAIN 1..199
FT /note="Putative nitrilase-like protein NIT1"
FT /id="PRO_0000204049"
FT DOMAIN 2..199
FT /note="CN hydrolase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT ACT_SITE 44
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT ACT_SITE 135
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT ACT_SITE 169
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00054,
FT ECO:0000255|PROSITE-ProRule:PRU10105"
FT CONFLICT 4
FT /note="H -> Y (in Ref. 1; CAA46923)"
FT /evidence="ECO:0000305"
FT CONFLICT 106
FT /note="V -> A (in Ref. 2; AAS56562)"
FT /evidence="ECO:0000305"
FT CONFLICT 173
FT /note="M -> I (in Ref. 1; CAA46923)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 199 AA; 21620 MW; B04D68EAF293B333 CRC64;
MAKHIVAALQ IGSCPGSTKD TLKKILSYEK EIKESGAKLV VIPEATLGGY PKGSNFGVYL
GYRLQEGREE YAKYLAEAIE IGNGEKYPEI SQLCALSKAT DASLCVGCIE RDGTTLYCTM
VYIDPKDGYV GKHRKLMPTA GERLIWGQGD GSTLPVVDTA AGKIGGAICW ENMMPLLRYA
MYKKGVEIWC APTVDARPI
//
